Experiment set2S415 for Phocaeicola vulgatus CL09T03C04

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D-Galactose carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Galactose (20 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 24-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya Tripathi on 3-Jul-23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 14 genes in this experiment

For carbon source D-Galactose in Phocaeicola vulgatus CL09T03C04

For carbon source D-Galactose across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 1
Entner-Doudoroff Pathway 1
Oxidative stress 1
Pentose phosphate pathway 1
Sialic Acid Metabolism 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 1
pentose phosphate pathway (oxidative branch) I 3 3 1
N-acetylglucosamine degradation II 3 3 1
D-galactosamine and N-acetyl-D-galactosamine degradation 4 2 1
cytosolic NADPH production (yeast) 5 3 1
N-acetyl-D-galactosamine degradation 5 2 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 3 1
formaldehyde oxidation I 6 3 1
L-lysine biosynthesis VI 7 7 1
pentose phosphate pathway 8 8 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 3 1
chitin derivatives degradation 8 3 1
Entner-Doudoroff pathway I 9 8 1
L-lysine biosynthesis I 9 6 1
L-lysine biosynthesis II 9 6 1
peptidoglycan recycling I 14 6 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 12 1
superpathway of glucose and xylose degradation 17 16 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
heterolactic fermentation 18 14 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 12 1
superpathway of N-acetylneuraminate degradation 22 17 1
aspartate superpathway 25 19 1