Experiment set2S404 for Rhodopseudomonas palustris CGA009

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NFM_No_Mo_Plus_VCl3 with Sodium acetate trihydrate

Group: sulfur source
Media: NFM_No_Mo_Plus_VCl3 + Sodium acetate trihydrate (20 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Fixen on 9/5/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, 10 uM vanadium chloride, UW concentrated base no Mo (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 6 genes in this experiment

For sulfur source Sodium acetate trihydrate in Rhodopseudomonas palustris CGA009

For sulfur source Sodium acetate trihydrate across organisms

SEED Subsystems

Subsystem #Specific
Nitrogen fixation 4

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
nitrogen fixation I (ferredoxin) 1 1 1
FeMo cofactor biosynthesis 6 2 1