Experiment set2S385 for Rhodopseudomonas palustris CGA009

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Dimethylsulfoniopropionate hydrochloride sulfur source

Group: sulfur source
Media: NoS + Sodium acetate trihydrate (20 mM) + Dimethylsulfoniopropionate hydrochloride (1 mM) + Light intensity (30 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_Fixen on 9/3/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium chloride, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base no sulfur (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium chloride hexahydrate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) chloride tetrahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc chloride, 0.000154 g/L Manganese (II) chloride tetrahydrate, 3.92e-05 g/L copper (II) chloride dihydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 13 genes in this experiment

For sulfur source Sodium acetate trihydrate in Rhodopseudomonas palustris CGA009

For sulfur source Sodium acetate trihydrate across organisms

SEED Subsystems

Subsystem #Specific
Alkanesulfonate assimilation 4
Alkanesulfonates Utilization 4
Nitrogen fixation 2
Conserved gene cluster possibly involved in RNA metabolism 1
Cysteine Biosynthesis 1
Methionine Biosynthesis 1
Putative sulfate assimilation cluster 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
nitrogen fixation I (ferredoxin) 1 1 1
L-cysteine biosynthesis I 2 2 1
two-component alkanesulfonate monooxygenase 2 1 1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) 4 2 1
N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis 4 1 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III 5 3 1
seleno-amino acid biosynthesis (plants) 5 3 1
dimethyl sulfide degradation II (oxidation) 5 2 1
D-cycloserine biosynthesis 6 1 1
L-cysteine biosynthesis VI (reverse transsulfuration) 7 3 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
superpathway of seleno-compound metabolism 19 8 1