Experiment set2S359 for Escherichia coli Nissle 1917
Phi92
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_Nissle_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 01/14/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 9 genes in this experiment
For phage Phage in Escherichia coli Nissle 1917
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biogenesis of c-type cytochromes | 1 |
| Periplasmic disulfide interchange | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| cellulose biosynthesis | 1 | 1 | 1 |
| periplasmic disulfide bond reduction | 2 | 2 | 1 |
| cytochrome c biogenesis (system I type) | 11 | 11 | 1 |