Experiment set2S334 for Escherichia coli Nissle 1917

Bas21

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_Nissle_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 01/14/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 8 genes in this experiment

For phage Phage in Escherichia coli Nissle 1917

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Maltose and Maltodextrin Utilization	4
Glycine and Serine Utilization	2
Glycine cleavage system	2
Photorespiration (oxidative C2 cycle)	2
Trehalose Uptake and Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine biosynthesis II	3	3	2
glycine cleavage	3	3	2