Experiment set2S172 for Escherichia coli Nissle 1917

L-Rhamnose

Group: carbon source
Media: M9_plus_L-Rhamnose + L-Rhamnose (20 mM)
Culturing: Ecoli_Nissle_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/2024

Specific Phenotypes

For 13 genes in this experiment

For carbon source L-Rhamnose in Escherichia coli Nissle 1917

For carbon source L-Rhamnose across organisms

SEED Subsystems

Subsystem	#Specific
L-rhamnose utilization	6
Ammonia assimilation	1
Aromatic amino acid degradation	1
Fermentations: Mixed acid	1
Iron acquisition in Vibrio	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1
Siderophore Aerobactin	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Pentose and glucuronate interconversions
• Tryptophan metabolism
• Pyruvate metabolism
• Carbon fixation in photosynthetic organisms
• Reductive carboxylate cycle (CO2 fixation)
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-tryptophan degradation II (via pyruvate)	3	3	3
L-serine degradation	3	3	2
D-serine degradation	3	3	2
L-cysteine degradation II	3	2	2
L-rhamnose degradation I	5	5	3
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
L-methionine biosynthesis II	6	5	2
glycine betaine degradation III	7	4	2
superpathway of fucose and rhamnose degradation	12	12	3
L-lyxose degradation	4	4	1
glycine betaine degradation I	8	4	2
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	3	2
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
partial TCA cycle (obligate autotrophs)	8	7	1
nitrogen remobilization from senescing leaves	8	5	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	8	1
purine nucleobases degradation II (anaerobic)	24	17	2
formaldehyde assimilation I (serine pathway)	13	8	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
mixed acid fermentation	16	16	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
ethene biosynthesis V (engineered)	25	18	1
superpathway of pentose and pentitol degradation	42	18	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	22	1