Experiment set2S161 for Escherichia coli Nissle 1917

Casamino-acids

Group: carbon source
Media: M9_plus_Casamino-acids + Casamino-acids (5 mg/mL)
Culturing: Ecoli_Nissle_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/2024

Specific Phenotypes

For 4 genes in this experiment

For carbon source Casamino-acids in Escherichia coli Nissle 1917

For carbon source Casamino-acids across organisms

SEED Subsystems

Subsystem	#Specific
De Novo Purine Biosynthesis	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Thiamin biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Alanine and aspartate metabolism
• One carbon pool by folate
• Thiamine metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate	2	2	1
thiamine diphosphate biosynthesis I (E. coli)	2	2	1
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I	2	2	1
L-glutamate degradation II	2	2	1
thiamine diphosphate biosynthesis II (Bacillus)	2	2	1
L-aspartate degradation III (anaerobic)	3	3	1
L-aspartate degradation II (aerobic)	3	3	1
thiamine diphosphate salvage V	3	3	1
thiamine diphosphate biosynthesis IV (eukaryotes)	3	1	1
thiamine diphosphate biosynthesis III (Staphylococcus)	3	1	1
superpathway of thiamine diphosphate biosynthesis III (eukaryotes)	7	3	2
superpathway of L-aspartate and L-asparagine biosynthesis	4	4	1
superpathway of thiamine diphosphate biosynthesis I	10	10	2
thiamine diphosphate salvage II	5	5	1
5-aminoimidazole ribonucleotide biosynthesis I	5	5	1
superpathway of thiamine diphosphate biosynthesis II	11	9	2
superpathway of 5-aminoimidazole ribonucleotide biosynthesis	6	5	1
L-canavanine degradation II	6	1	1
thiamine diphosphate salvage IV (yeast)	7	4	1
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast)	8	4	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	11	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1