Experiment set2S148 for Escherichia coli Nissle 1917
D-Glucose
Group: carbon sourceMedia: M9_plus_D-Glucose + D-Glucose (20 mM)
Culturing: Ecoli_Nissle_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/2024
Specific Phenotypes
For 5 genes in this experiment
For carbon source D-Glucose in Escherichia coli Nissle 1917
For carbon source D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Acetoin, butanediol metabolism | 1 |
| Branched-Chain Amino Acid Biosynthesis | 1 |
| Phosphate metabolism | 1 |
| Proteolysis in bacteria, ATP-dependent | 1 |
| Stringent Response, (p)ppGpp metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Valine, leucine and isoleucine biosynthesis
- Lysine degradation
- Butanoate metabolism
- C5-Branched dibasic acid metabolism
- Pantothenate and CoA biosynthesis
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| polyphosphate metabolism | 2 | 2 | 1 |
| pyruvate fermentation to (R)-acetoin II | 2 | 1 | 1 |
| pyruvate fermentation to (S)-acetoin | 3 | 3 | 1 |
| pyruvate fermentation to (R)-acetoin I | 3 | 2 | 1 |
| aerobactin biosynthesis | 4 | 4 | 1 |
| L-valine biosynthesis | 4 | 4 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| superpathway of (R,R)-butanediol biosynthesis | 5 | 2 | 1 |
| ppGpp metabolism | 6 | 6 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| superpathway of 2,3-butanediol biosynthesis | 6 | 3 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| L-isoleucine biosynthesis III | 7 | 4 | 1 |
| L-isoleucine biosynthesis II | 8 | 4 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 2 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| superpathway of L-threonine metabolism | 18 | 15 | 1 |