Culturing: Ecoli_Nissle_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
| Pathway | #Steps | #Present | #Specific |
|---|
| aerobactin biosynthesis | 4 | 4 | 4 |
| thiamine diphosphate biosynthesis II (Bacillus) | 2 | 2 | 1 |
| thiamine diphosphate biosynthesis I (E. coli) | 2 | 2 | 1 |
| NAD phosphorylation and transhydrogenation | 2 | 2 | 1 |
| CO2 fixation into oxaloacetate (anaplerotic) | 2 | 2 | 1 |
| polyphosphate metabolism | 2 | 2 | 1 |
| pyruvate fermentation to (R)-acetoin II | 2 | 1 | 1 |
| thiamine diphosphate salvage V | 3 | 3 | 1 |
| pyruvate fermentation to (S)-acetoin | 3 | 3 | 1 |
| NAD phosphorylation and dephosphorylation | 3 | 3 | 1 |
| pyruvate fermentation to (R)-acetoin I | 3 | 2 | 1 |
| thiamine diphosphate biosynthesis IV (eukaryotes) | 3 | 1 | 1 |
| thiamine diphosphate biosynthesis III (Staphylococcus) | 3 | 1 | 1 |
| L-lyxose degradation | 4 | 4 | 1 |
| heme b biosynthesis I (aerobic) | 4 | 4 | 1 |
| L-arabinose degradation I | 4 | 4 | 1 |
| heme b biosynthesis V (aerobic) | 4 | 4 | 1 |
| L-valine biosynthesis | 4 | 4 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 6 | 3 |
| superpathway of thiamine diphosphate biosynthesis I | 10 | 10 | 2 |
| L-ornithine biosynthesis I | 5 | 5 | 1 |
| thiamine diphosphate salvage II | 5 | 5 | 1 |
| L-arginine degradation II (AST pathway) | 5 | 5 | 1 |
| L-ascorbate degradation I (bacterial, anaerobic) | 5 | 5 | 1 |
| pentose phosphate pathway (non-oxidative branch) I | 5 | 5 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| superpathway of (R,R)-butanediol biosynthesis | 5 | 2 | 1 |
| thiazole component of thiamine diphosphate biosynthesis I | 6 | 6 | 1 |
| superpathway of heme b biosynthesis from uroporphyrinogen-III | 6 | 6 | 1 |
| ppGpp metabolism | 6 | 6 | 1 |
| NAD(P)/NADPH interconversion | 6 | 4 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| superpathway of 2,3-butanediol biosynthesis | 6 | 3 | 1 |
| L-ascorbate degradation II (bacterial, aerobic) | 7 | 7 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| C4 photosynthetic carbon assimilation cycle, NADP-ME type | 7 | 4 | 1 |
| thiamine diphosphate salvage IV (yeast) | 7 | 4 | 1 |
| L-isoleucine biosynthesis III | 7 | 4 | 1 |
| superpathway of thiamine diphosphate biosynthesis III (eukaryotes) | 7 | 3 | 1 |
| pentose phosphate pathway | 8 | 8 | 1 |
| superpathway of heme b biosynthesis from glycine | 8 | 7 | 1 |
| partial TCA cycle (obligate autotrophs) | 8 | 7 | 1 |
| nitrogen remobilization from senescing leaves | 8 | 5 | 1 |
| L-isoleucine biosynthesis II | 8 | 4 | 1 |
| thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) | 8 | 4 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 2 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| L-lysine biosynthesis I | 9 | 9 | 1 |
| L-arginine biosynthesis I (via L-ornithine) | 9 | 9 | 1 |
| L-arginine biosynthesis III (via N-acetyl-L-citrulline) | 9 | 8 | 1 |
| formaldehyde assimilation II (assimilatory RuMP Cycle) | 9 | 8 | 1 |
| 3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent) | 9 | 3 | 1 |
| 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent) | 9 | 3 | 1 |
| superpathway of heme b biosynthesis from glutamate | 10 | 10 | 1 |
| L-arginine biosynthesis II (acetyl cycle) | 10 | 9 | 1 |
| Rubisco shunt | 10 | 9 | 1 |
| peptidoglycan recycling II | 10 | 7 | 1 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 9 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 8 | 1 |
| mycobactin biosynthesis | 11 | 4 | 1 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 12 | 1 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | 12 | 10 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 8 | 1 |
| peptidoglycan recycling I | 14 | 14 | 1 |
| C4 photosynthetic carbon assimilation cycle, PEPCK type | 14 | 9 | 1 |
| Bifidobacterium shunt | 15 | 13 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 11 | 1 |
| mixed acid fermentation | 16 | 16 | 1 |
| superpathway of glucose and xylose degradation | 17 | 17 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 16 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 18 | 1 |
| superpathway of L-threonine metabolism | 18 | 15 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 9 | 1 |
| aspartate superpathway | 25 | 24 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 18 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 22 | 1 |