Experiment set2S114 for Castellaniella sp019104865 MT123

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Anaerobic; Cobalt chloride hexahydrate 2 uM

Group: anaerobic metal stress
Media: CRBTM_UGA_Anaerobic_MOPS + Cobalt chloride hexahydrate (2 uM), pH=7.5
Culturing: Castellaniella_MT123_ML3, tube, Anaerobic, at 30 (C)
By: Mike Adams lab on 6/27/23
Media components: 3.74 mM Ammonium chloride, 1.34 mM Potassium Chloride, 2 mM Magnesium Sulfate Heptahydrate, 0.1 mM Calcium chloride dihydrate, 0.17 mM Sodium Chloride, 20 mM Sodium D,L-Lactate, 40 mM 3-(N-morpholino)propanesulfonic acid, 10 mM Sodium nitrate, ATCC Wolfe's mineral mix (5 mg/L EDTA, 3 mg/L Magnesium Sulfate Heptahydrate, 5 mg/L Manganese (II) sulfate monohydrate, 10 mg/L Sodium Chloride, 1 mg/L Iron (II) sulfate heptahydrate, 1 mg/L Cobalt(II) nitrate hexahydrate, 1 mg/L Calcium chloride dihydrate, 1 mg/L Zinc sulfate heptahydrate, 0.1 mg/L Copper (II) sulfate pentahydrate, 0.1 mg/L Aluminum potassium sulfate dodecahydrate, 0.1 mg/L Boric Acid, 0.1 mg/L Sodium Molybdate Dihydrate, 0.01 mg/L Sodium selenite pentahydrate, 0.1 mg/L Sodium tungstate dihydrate, 0.2 mg/L Nickel (II) chloride hexahydrate), ATCC Wolfe's vitamin mix (20 ug/L Folic Acid, 100 ug/L Pyridoxine HCl, 50 ug/L Riboflavin, 20 ug/L biotin, 50 ug/L Thiamine HCl, 50 ug/L Nicotinic Acid, 50 ug/L calcium pantothenate, 1 ug/L Cyanocobalamin, 50 ug/L 4-Aminobenzoic acid, 50 ug/L Lipoic acid)

Specific Phenotypes

None in this experiment

For Castellaniella sp019104865 MT123 in anaerobic metal stress experiments

For anaerobic metal stress Cobalt chloride hexahydrate across organisms