Experiment set2S106 for Rhodanobacter denitrificans MT42
R2A_PIPES pH 4.25
Group: pHMedia: R2A, pH=4.3
Culturing: Rhodanobacter_MT42_ML2, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
By: Hans and Hira on 1/10/25
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| NAD and NADP cofactor biosynthesis global | 1 |
| Quinolinic acid and its derivatives | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde | 4 | 4 | 1 |
| NAD de novo biosynthesis II (from tryptophan) | 9 | 9 | 2 |
| L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde | 5 | 5 | 1 |
| superpathway of NAD biosynthesis in eukaryotes | 14 | 11 | 2 |
| 2-nitrobenzoate degradation I | 7 | 4 | 1 |
| L-tryptophan degradation IX | 12 | 8 | 1 |
| L-tryptophan degradation XII (Geobacillus) | 12 | 7 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 11 | 1 |
| L-tryptophan degradation XI (mammalian, via kynurenine) | 23 | 12 | 1 |