Experiment set2IT095 for Rhodanobacter denitrificans FW104-10B01

R2A with Sodium nitrite 50 mM

Group: stress
Media: R2A + Sodium nitrite (50 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 02/27/2021
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 6 genes in this experiment

For stress Sodium nitrite in Rhodanobacter denitrificans FW104-10B01

For stress Sodium nitrite across organisms

SEED Subsystems

Subsystem	#Specific
Glutathione: Biosynthesis and gamma-glutamyl cycle	1
Mannose Metabolism	1
Soluble cytochromes and functionally related electron carriers	1
Ton and Tol transport systems	1
Trehalose Uptake and Utilization	1
Utilization of glutathione as a sulphur source	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Taurine and hypotaurine metabolism
• Selenoamino acid metabolism
• Cyanoamino acid metabolism
• Glutathione metabolism
• Arachidonic acid metabolism
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glutathione degradation (DUG pathway)	2	1	1
diphenyl ethers degradation	4	2	1
biphenyl degradation	5	2	1
peptido-conjugates in tissue regeneration biosynthesis	17	3	3
γ-glutamyl cycle	6	3	1
leukotriene biosynthesis	6	1	1
hypoglycin biosynthesis	14	4	1