Experiment set2IT086 for Rhodanobacter denitrificans FW104-10B01

Compare to:

R2A with Sodium m-arsenite 0.078125 mM

Group: stress
Media: R2A + Sodium m-arsenite (0.078125 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 2/27/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 15 genes in this experiment

For stress Sodium m-arsenite in Rhodanobacter denitrificans FW104-10B01

For stress Sodium m-arsenite across organisms

SEED Subsystems

Subsystem #Specific
Arsenic resistance 3
Lipoic acid metabolism 2
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Glutathione: Non-redox reactions 1
Methylglyoxal Metabolism 1
Serine-glyoxylate cycle 1
TCA Cycle 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-aspartate degradation I 1 1 1
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
L-aspartate biosynthesis 1 1 1
malate/L-aspartate shuttle pathway 2 2 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
atromentin biosynthesis 2 1 1
L-glutamate degradation II 2 1 1
L-tyrosine degradation II 2 1 1
lipoate salvage I 2 1 1
L-tyrosine biosynthesis I 3 3 1
L-phenylalanine biosynthesis I 3 3 1
L-asparagine degradation III (mammalian) 3 3 1
TCA cycle VIII (Chlamydia) 6 5 2
methylglyoxal degradation I 3 2 1
methylglyoxal degradation VIII 3 2 1
(R)-cysteate degradation 3 1 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
sulfolactate degradation III 3 1 1
L-phenylalanine degradation II (anaerobic) 3 1 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 3 1
L-phenylalanine degradation III 4 2 1
L-tyrosine degradation III 4 2 1
lipoate salvage II 4 1 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
L-tyrosine degradation I 5 4 1
trans-4-hydroxy-L-proline degradation I 5 3 1
superpathway of plastoquinol biosynthesis 5 2 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 7 2
superpathway of L-threonine biosynthesis 6 6 1
superpathway of sulfolactate degradation 6 2 1
coenzyme M biosynthesis II 6 1 1
anaerobic energy metabolism (invertebrates, cytosol) 7 7 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 2
pyruvate fermentation to propanoate I 7 3 1
incomplete reductive TCA cycle 7 3 1
partial TCA cycle (obligate autotrophs) 8 7 1
superpathway of methylglyoxal degradation 8 4 1
superpathway of anaerobic energy metabolism (invertebrates) 17 13 2
superpathway of aromatic amino acid biosynthesis 18 18 2
TCA cycle V (2-oxoglutarate synthase) 9 8 1
TCA cycle II (plants and fungi) 9 8 1
TCA cycle IV (2-oxoglutarate decarboxylase) 9 7 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
TCA cycle VII (acetate-producers) 9 7 1
TCA cycle VI (Helicobacter) 9 6 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 2 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
TCA cycle III (animals) 10 9 1
TCA cycle I (prokaryotic) 10 8 1
anaerobic energy metabolism (invertebrates, mitochondrial) 10 6 1
rosmarinic acid biosynthesis I 10 1 1
reductive TCA cycle I 11 6 1
L-glutamate degradation VIII (to propanoate) 11 3 1
(S)-reticuline biosynthesis I 11 1 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 11 1
superpathway of glyoxylate bypass and TCA 12 10 1
reductive TCA cycle II 12 5 1
indole-3-acetate biosynthesis II 12 3 1
superpathway of L-isoleucine biosynthesis I 13 13 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 5 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 1
superpathway of rosmarinic acid biosynthesis 14 1 1
mixed acid fermentation 16 12 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
methylaspartate cycle 19 11 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 19 1
aspartate superpathway 25 23 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 1 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 25 2
superpathway of chorismate metabolism 59 40 2