Experiment set2IT081 for Rhodanobacter denitrificans FW104-10B01

R2A with Potassium chromate 0.0048828125 mM

Group: stress
Media: R2A + Potassium chromate (0.004882813 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 2/27/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 3 genes in this experiment

For stress Potassium chromate in Rhodanobacter denitrificans FW104-10B01

For stress Potassium chromate across organisms

SEED Subsystems

Subsystem	#Specific
Central meta-cleavage pathway of aromatic compound degradation	1
Cysteine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Benzoate degradation via hydroxylation
• Fluorene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-chlorocatechol degradation III (meta pathway)	4	2	1
catechol degradation to 2-hydroxypentadienoate II	4	1	1
orthanilate degradation	5	2	1
protocatechuate degradation III (para-cleavage pathway)	5	2	1
2,3-dihydroxybenzoate degradation	5	1	1
4-amino-3-hydroxybenzoate degradation	6	2	1
catechol degradation II (meta-cleavage pathway)	7	1	1
meta cleavage pathway of aromatic compounds	10	2	1
2,2'-dihydroxybiphenyl degradation	10	1	1
toluene degradation IV (aerobic) (via catechol)	13	2	1
superpathway of aerobic toluene degradation	30	7	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	8	1