Experiment set2IT080 for Burkholderia phytofirmans PsJN

LB with Nickel (II) chloride hexahydrate 1 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (1 mM)
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.1 generations
By: Mark on 6/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: BFirm_SP2 A2

Specific Phenotypes

For 10 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Burkholderia phytofirmans PsJN

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Iron	2
Campylobacter Iron Metabolism	1
Iron acquisition in Vibrio	1
Methionine Biosynthesis	1
Soluble cytochromes and functionally related electron carriers	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Cysteine metabolism
• Selenoamino acid metabolism
• Nitrogen metabolism
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
hydrogen sulfide biosynthesis II (mammalian)	6	1	1