Experiment set2IT078 for Rhodanobacter denitrificans FW104-10B01
R2A with Chromium(III) Chloride Hexahydrate 0.15625 mM
Group: stressMedia: R2A + Chromium(III) Chloride Hexahydrate (0.15625 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 2/27/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Specific Phenotypes
For 14 genes in this experiment
For stress Chromium(III) Chloride Hexahydrate in Rhodanobacter denitrificans FW104-10B01
For stress Chromium(III) Chloride Hexahydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Peptidoglycan biosynthesis
- Purine metabolism
- Lipopolysaccharide biosynthesis
- Carotenoid biosynthesis - General
- Fructose and mannose metabolism
- Puromycin biosynthesis
- Lysine degradation
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- High-mannose type N-glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Nucleotide sugars metabolism
- Keratan sulfate biosynthesis
- Glycerolipid metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- alpha-Linolenic acid metabolism
- Sphingolipid metabolism
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Trinitrotoluene degradation
- Biotin metabolism
- Folate biosynthesis
- Porphyrin and chlorophyll metabolism
- Zeatin biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Flavone and flavonol biosynthesis
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| β-alanine degradation III | 2 | 1 | 1 |
| uracil degradation III | 5 | 3 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 11 | 2 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 11 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 10 | 2 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 10 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 1 |
| 3-hydroxypropanoate cycle | 13 | 7 | 1 |
| glyoxylate assimilation | 13 | 5 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 10 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 10 | 1 |
| superpathway of the 3-hydroxypropanoate cycle | 18 | 7 | 1 |