Experiment set2IT078 for Rhodanobacter denitrificans FW104-10B01

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R2A with Chromium(III) Chloride Hexahydrate 0.15625 mM

Group: stress
Media: R2A + Chromium(III) Chloride Hexahydrate (0.15625 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 02/27/2021
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 17 genes in this experiment

For stress Chromium(III) Chloride Hexahydrate in Rhodanobacter denitrificans FW104-10B01

For stress Chromium(III) Chloride Hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Campylobacter Iron Metabolism 2
Peptidoglycan Biosynthesis 2
Calvin-Benson cycle 1
DNA-replication 1
DNA repair, bacterial UvrD and related helicases 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Hemin transport system 1
Hfl operon 1
Polyadenylation bacterial 1
Pyrimidine utilization 1
Ton and Tol transport systems 1
Type IV pilus 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
β-alanine degradation III 2 1 1
uracil degradation III 5 3 1
peptidoglycan biosynthesis II (staphylococci) 17 11 2
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 11 2
peptidoglycan biosynthesis V (β-lactam resistance) 17 10 2
sucrose biosynthesis I (from photosynthesis) 9 7 1
glycolysis II (from fructose 6-phosphate) 11 11 1
gluconeogenesis III 12 10 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 10 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 4 1
glycolysis I (from glucose 6-phosphate) 13 12 1
gluconeogenesis I 13 11 1
Calvin-Benson-Bassham cycle 13 9 1
3-hydroxypropanoate cycle 13 7 1
glyoxylate assimilation 13 5 1
peptidoglycan biosynthesis III (mycobacteria) 15 10 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 16 1
oxygenic photosynthesis 17 10 1
superpathway of hexitol degradation (bacteria) 18 14 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 1
superpathway of the 3-hydroxypropanoate cycle 18 7 1
superpathway of anaerobic sucrose degradation 19 16 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 16 1
ethene biosynthesis V (engineered) 25 17 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 1
1-butanol autotrophic biosynthesis (engineered) 27 18 1