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Experiment set2IT078 for
Methanococcus maripaludis JJ
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Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS04415
+1.8
11.3
hypothetical protein
compare
MMJJ
_RS01785
+0.8
1.4
50S ribosomal protein L14e
compare
MMJJ
_RS04720
+0.8
1.1
IMP dehydrogenase
compare
MMJJ
_RS07030
+0.7
5.9
hypothetical protein
compare
MMJJ
_RS08775
+0.6
4.8
PfkB family carbohydrate kinase
compare
MMJJ
_RS08785
+0.6
3.7
D-sedoheptulose 7-phosphate isomerase
compare
MMJJ
_RS08765
+0.6
5.3
glycosyltransferase family 9 protein
compare
MMJJ
_RS01590
+0.6
1.0
adenine phosphoribosyltransferase
compare
MMJJ
_RS08715
+0.6
3.2
NAD-dependent epimerase/dehydratase family protein
compare
MMJJ
_RS06800
+0.5
3.2
prefoldin subunit alpha
compare
MMJJ
_RS09295
+0.5
0.7
hypothetical protein
compare
MMJJ
_RS05820
+0.5
2.3
DUF2304 domain-containing protein
compare
MMJJ
_RS08760
+0.5
3.9
glycosyltransferase family 9 protein
compare
MMJJ
_RS05870
+0.5
1.7
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMJJ
_RS08730
+0.5
4.1
glycosyltransferase family 4 protein
compare
MMJJ
_RS07280
+0.5
3.3
phosphomannomutase/phosphoglucomutase
compare
MMJJ
_RS05760
+0.5
4.1
flagellar protein F
compare
MMJJ
_RS06130
+0.5
2.7
hypothetical protein
compare
MMJJ
_RS04480
+0.4
1.8
hypothetical protein
compare
MMJJ
_RS08040
+0.4
3.5
NAD(P)/FAD-dependent oxidoreductase
compare
MMJJ
_RS00420
+0.4
2.8
CBS domain-containing protein
compare
MMJJ
_RS05510
+0.4
3.3
archaellum operon transcriptional activator EarA family protein
compare
MMJJ
_RS08720
+0.4
3.6
UDP-glucose/GDP-mannose dehydrogenase family protein
compare
MMJJ
_RS05740
+0.4
3.7
archaellar assembly protein FlaJ
compare
MMJJ
_RS09325
+0.4
2.7
hypothetical protein
compare
MMJJ
_RS08515
+0.4
1.9
secondary thiamine-phosphate synthase enzyme YjbQ
compare
MMJJ
_RS06665
+0.4
2.2
DUF3236 domain-containing protein
compare
MMJJ
_RS06975
+0.4
1.3
protein translocase SEC61 complex subunit gamma
compare
MMJJ
_RS08750
+0.4
3.5
glycosyltransferase
compare
MMJJ
_RS00525
+0.4
1.0
Dabb family protein
compare
MMJJ
_RS05750
+0.4
3.2
flagellar accessory protein FlaH
compare
MMJJ
_RS07680
+0.4
3.1
glucose-6-phosphate isomerase
compare
MMJJ
_RS05745
+0.4
3.3
type II/IV secretion system ATPase subunit
compare
MMJJ
_RS05790
+0.4
2.8
flagellin
compare
MMJJ
_RS03370
+0.4
1.7
hypothetical protein
compare
MMJJ
_RS05915
+0.4
1.2
multiprotein bridging factor aMBF1
compare
MMJJ
_RS05505
+0.4
3.6
hypothetical protein
compare
MMJJ
_RS09370
+0.4
1.4
heavy-metal-associated domain-containing protein
compare
MMJJ
_RS05560
+0.3
1.5
30S ribosomal protein S27e
compare
MMJJ
_RS08780
+0.3
2.1
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
compare
MMJJ
_RS05755
+0.3
2.8
flagellar protein G
compare
MMJJ
_RS08710
+0.3
2.3
UTP--glucose-1-phosphate uridylyltransferase GalU
compare
MMJJ
_RS06740
+0.3
2.1
energy-coupling factor ABC transporter substrate-binding protein
compare
MMJJ
_RS01100
+0.3
0.8
helix-turn-helix domain-containing protein
compare
MMJJ
_RS00415
+0.3
1.6
DedA family protein
compare
MMJJ
_RS08580
+0.3
1.7
YkgJ family cysteine cluster protein
compare
MMJJ
_RS06765
+0.3
2.6
hypothetical protein
compare
MMJJ
_RS07690
+0.3
2.3
glycosyltransferase family 4 protein
compare
MMJJ
_RS03835
+0.3
1.7
DUF504 domain-containing protein
compare
MMJJ
_RS05785
+0.3
2.2
flagellin
compare
MMJJ
_RS06190
+0.3
2.4
7-cyano-7-deazaguanine synthase
compare
MMJJ
_RS07610
+0.3
1.8
DUF2119 domain-containing protein
compare
MMJJ
_RS00950
+0.3
1.8
DUF111 family protein
compare
MMJJ
_RS03850
+0.3
1.7
nucleoside-diphosphate kinase
compare
MMJJ
_RS06205
+0.3
2.0
EamA family transporter
compare
MMJJ
_RS06810
+0.3
2.3
L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
compare
MMJJ
_RS06430
+0.3
2.1
haloacid dehalogenase
compare
MMJJ
_RS03410
+0.3
1.0
hypothetical protein
compare
MMJJ
_RS06735
+0.3
2.0
cobalt ECF transporter T component CbiQ
compare
MMJJ
_RS04375
+0.3
1.3
hypothetical protein
compare
MMJJ
_RS09380
+0.3
1.2
hypothetical protein
compare
MMJJ
_RS08325
+0.2
0.5
glycosyltransferase family 2 protein
compare
MMJJ
_RS09255
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS02965
+0.2
1.5
RimK-like ATPgrasp N-terminal domain-containing protein
compare
MMJJ
_RS03185
+0.2
0.5
alanine--tRNA ligase
compare
MMJJ
_RS06480
+0.2
1.8
hypothetical protein
compare
MMJJ
_RS06505
+0.2
1.1
prephenate dehydratase
compare
MMJJ
_RS04165
+0.2
2.0
sodium:solute symporter
compare
MMJJ
_RS04175
+0.2
1.6
hypothetical protein
compare
MMJJ
_RS03360
+0.2
0.4
hypothetical protein
compare
MMJJ
_RS06770
+0.2
2.0
hypothetical protein
compare
MMJJ
_RS05115
+0.2
1.0
P-II family nitrogen regulator
compare
MMJJ
_RS06105
+0.2
1.8
DUF99 family protein
compare
MMJJ
_RS04585
+0.2
1.7
phenylacetate--CoA ligase
compare
MMJJ
_RS07040
+0.2
0.3
preprotein translocase subunit SecY
compare
MMJJ
_RS07865
+0.2
1.8
zinc ribbon domain-containing protein
compare
MMJJ
_RS06710
+0.2
1.8
TldD/PmbA family protein
compare
MMJJ
_RS03070
+0.2
1.1
MetS family NSS transporter small subunit
compare
MMJJ
_RS02290
+0.2
1.6
MarR family transcriptional regulator
compare
MMJJ
_RS05890
+0.2
1.8
molybdate ABC transporter substrate-binding protein
compare
MMJJ
_RS06585
+0.2
1.5
alanine racemase
compare
MMJJ
_RS07595
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS06470
+0.2
1.5
tripartite tricarboxylate transporter permease
compare
MMJJ
_RS05980
+0.2
1.6
DUF5400 domain-containing protein
compare
MMJJ
_RS04780
+0.2
1.0
nucleic acid-binding protein
compare
MMJJ
_RS08705
+0.2
1.6
AEC family transporter
compare
MMJJ
_RS02245
+0.2
1.4
DUF432 domain-containing protein
compare
MMJJ
_RS00300
+0.2
1.4
chemotaxis protein CheC
compare
MMJJ
_RS03350
+0.2
0.6
hypothetical protein
compare
MMJJ
_RS01060
+0.2
1.4
ABC transporter ATP-binding protein
compare
MMJJ
_RS07220
+0.2
1.6
coenzyme F420 hydrogenase subunit beta
compare
MMJJ
_RS07450
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS03545
+0.2
1.5
flagella protein F
compare
MMJJ
_RS08830
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS05985
+0.2
1.3
ABC transporter substrate-binding protein
compare
MMJJ
_RS06520
+0.2
1.5
TldD/PmbA family protein
compare
MMJJ
_RS04215
+0.2
1.6
DMT family transporter
compare
MMJJ
_RS06455
+0.2
1.5
YeeE/YedE family protein
compare
MMJJ
_RS06790
+0.2
1.6
nucleotidyltransferase family protein
compare
MMJJ
_RS06160
+0.2
1.4
stage II sporulation protein M
compare
MMJJ
_RS06115
+0.2
1.5
pyruvate kinase
compare
MMJJ
_RS07235
+0.2
1.5
coenzyme F420 hydrogenase subunit alpha
compare
MMJJ
_RS03045
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS02860
+0.2
0.5
site-specific integrase
compare
MMJJ
_RS06045
+0.2
1.4
hypothetical protein
compare
MMJJ
_RS07795
+0.2
1.3
cupin domain-containing protein
compare
MMJJ
_RS05975
+0.2
1.3
sulfurtransferase TusA family protein
compare
MMJJ
_RS05200
+0.2
1.5
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMJJ
_RS07860
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS02345
+0.2
1.4
ATP-binding protein
compare
MMJJ
_RS02355
+0.2
1.5
DUF2798 domain-containing protein
compare
MMJJ
_RS07935
+0.2
0.3
protein fwdD
compare
MMJJ
_RS06640
+0.2
1.3
YfcE family phosphodiesterase
compare
MMJJ
_RS07210
+0.2
1.4
AmmeMemoRadiSam system protein B
compare
MMJJ
_RS02115
+0.2
1.3
class I SAM-dependent methyltransferase family protein
compare
MMJJ
_RS08345
+0.2
1.4
flavodoxin family protein
compare
MMJJ
_RS02110
+0.2
0.9
carboxymuconolactone decarboxylase family protein
compare
MMJJ
_RS02340
+0.2
1.1
SAM-dependent methyltransferase
compare
MMJJ
_RS07225
+0.2
1.3
coenzyme F420 hydrogenase subunit gamma
compare
MMJJ
_RS04690
+0.2
1.4
Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain
compare
MMJJ
_RS07230
+0.2
1.1
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS00115
+0.2
0.8
hypothetical protein
compare
MMJJ
_RS04755
+0.2
1.2
5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB
compare
MMJJ
_RS09265
+0.2
0.5
hypothetical protein
compare
MMJJ
_RS07825
+0.2
0.8
YciI family protein
compare
MMJJ
_RS04745
+0.2
1.1
cyclase family protein
compare
MMJJ
_RS06660
+0.2
1.1
DUF1188 domain-containing protein
compare
MMJJ
_RS05335
+0.2
0.9
heavy metal-binding domain-containing protein
compare
MMJJ
_RS06580
+0.2
1.3
alanine dehydrogenase
compare
MMJJ
_RS06720
+0.2
1.2
mechanosensitive ion channel family protein
compare
MMJJ
_RS03590
+0.1
1.0
restriction endonuclease
compare
MMJJ
_RS04300
+0.1
1.1
hypothetical protein
compare
MMJJ
_RS04180
+0.1
0.4
metalloregulator ArsR/SmtB family transcription factor
compare
MMJJ
_RS00790
+0.1
0.9
ribonuclease H family protein
compare
MMJJ
_RS06280
+0.1
1.3
hypothetical protein
compare
MMJJ
_RS03945
+0.1
1.1
mechanosensitive ion channel family protein
compare
MMJJ
_RS05235
+0.1
1.3
Flp pilus assembly complex ATPase component TadA
compare
MMJJ
_RS09230
+0.1
0.8
hypothetical protein
compare
MMJJ
_RS03690
+0.1
1.0
indolepyruvate oxidoreductase subunit beta
compare
MMJJ
_RS08370
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS04430
+0.1
1.2
purine NTPase
compare
MMJJ
_RS03385
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS08295
+0.1
1.2
iron ABC transporter substrate-binding protein
compare
MMJJ
_RS06550
+0.1
1.2
ABC transporter permease
compare
MMJJ
_RS06460
+0.1
1.0
cytochrome c
compare
MMJJ
_RS00400
+0.1
0.7
tautomerase family protein
compare
MMJJ
_RS06685
+0.1
1.2
S-layer protein
compare
MMJJ
_RS07025
+0.1
0.8
tRNA (cytidine(56)-2'-O)-methyltransferase
compare
MMJJ
_RS07385
+0.1
1.0
ribosomal protein S18-alanine N-acetyltransferase
compare
MMJJ
_RS06445
+0.1
1.2
hypothetical protein
compare
MMJJ
_RS05525
+0.1
0.8
GTP-binding protein
compare
MMJJ
_RS08490
+0.1
0.6
rubrerythrin family protein
compare
MMJJ
_RS07740
+0.1
1.0
DUF2334 domain-containing protein
compare
MMJJ
_RS05250
+0.1
0.9
HDIG domain-containing protein
compare
MMJJ
_RS06120
+0.1
1.0
DUF4013 domain-containing protein
compare
MMJJ
_RS01125
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS05585
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS05500
+0.1
1.2
hypothetical protein
compare
MMJJ
_RS03355
+0.1
1.0
hypothetical protein
compare
MMJJ
_RS07915
+0.1
0.9
CBS domain-containing protein
compare
MMJJ
_RS08365
+0.1
0.7
DsrE family protein
compare
MMJJ
_RS09390
+0.1
0.6
hypothetical protein
compare
MMJJ
_RS04250
+0.1
0.7
thioredoxin family protein
compare
MMJJ
_RS03750
+0.1
0.9
rubredoxin
compare
MMJJ
_RS00695
+0.1
1.0
P-II family nitrogen regulator
compare
MMJJ
_RS01840
+0.1
0.8
flavodoxin family protein
compare
MMJJ
_RS08885
+0.1
0.9
cysteine--tRNA ligase
compare
MMJJ
_RS01615
+0.1
0.6
MoaD/ThiS family protein
compare
MMJJ
_RS01525
+0.1
1.0
ketopantoate reductase family protein
compare
MMJJ
_RS01770
+0.1
0.7
TOBE domain-containing protein
compare
MMJJ
_RS06440
+0.1
0.8
DUF4870 domain-containing protein
compare
MMJJ
_RS01740
+0.1
0.9
ATP-binding protein
compare
MMJJ
_RS05240
+0.1
1.1
type II secretion system F family protein
compare
MMJJ
_RS05685
+0.1
0.8
class III signal peptide-containing protein
compare
MMJJ
_RS07480
+0.1
1.0
FAD-dependent oxidoreductase
compare
MMJJ
_RS01035
+0.1
0.5
hypothetical protein
compare
MMJJ
_RS01945
+0.1
0.6
cell division ATPase MinD
compare
MMJJ
_RS05400
+0.1
0.6
CoA-binding protein
compare
MMJJ
_RS01405
+0.1
0.2
proline--tRNA ligase
compare
MMJJ
_RS07270
+0.1
0.8
ribonuclease HII
compare
MMJJ
_RS03285
+0.1
0.5
gamma-glutamylcyclotransferase
compare
MMJJ
_RS06960
+0.1
1.0
CAP domain-containing protein
compare
MMJJ
_RS08075
+0.1
0.9
ATP-dependent DNA helicase
compare
MMJJ
_RS07835
+0.1
0.8
UPF0489 family protein
compare
MMJJ
_RS07630
+0.1
0.8
hypothetical protein
compare
MMJJ
_RS05970
+0.1
0.4
MJ0307 family thioredoxin
compare
MMJJ
_RS00870
+0.1
0.7
F420-nonreducing hydrogenase
compare
MMJJ
_RS05340
+0.1
0.8
AAA family ATPase
compare
MMJJ
_RS04750
+0.1
0.7
5,10-methenyltetrahydromethanopterin hydrogenase
compare
MMJJ
_RS07255
+0.1
1.0
hypothetical protein
compare
MMJJ
_RS06135
+0.1
0.8
50S ribosomal protein L11 methyltransferase
compare
MMJJ
_RS09355
+0.1
0.5
hypothetical protein
compare
MMJJ
_RS01505
+0.1
0.6
DUF2124 domain-containing protein
compare
MMJJ
_RS04785
+0.1
0.7
OB-fold nucleic acid binding domain-containing protein
compare
MMJJ
_RS06515
+0.1
0.8
ribonuclease III
compare
MMJJ
_RS05405
+0.1
0.8
DNA cytosine methyltransferase
compare
MMJJ
_RS07445
+0.1
0.7
RlmF-related methyltransferase
compare
MMJJ
_RS08015
+0.1
0.7
HAD family hydrolase
compare
MMJJ
_RS03885
+0.1
0.8
tetratricopeptide repeat protein
compare
MMJJ
_RS06950
+0.1
0.8
4-demethylwyosine synthase TYW1
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in rich t1 experiments