Experiment set2IT073 for Bifidobacterium breve UCC2003
CDM1 without Isoleucine
Group: nutrient dropoutMedia: CDM1 + no Isoleucine, pH=7
Culturing: Bifido_ML2, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Anthony Shiver on 12/10/2019
Growth plate: CDM1_nutrientdrop G1
Specific Phenotypes
For 4 genes in this experiment
For nutrient dropout no Isoleucine in Bifidobacterium breve UCC2003
For nutrient dropout no Isoleucine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Branched-Chain Amino Acid Biosynthesis | 2 |
| Acetoin, butanediol metabolism | 1 |
| Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine biosynthesis
- Pantothenate and CoA biosynthesis
- Biosynthesis of alkaloids derived from histidine and purine
- Pyruvate metabolism
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- C5-Branched dibasic acid metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-valine biosynthesis | 4 | 4 | 2 |
| pyruvate fermentation to (R)-acetoin II | 2 | 2 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 2 |
| L-isoleucine biosynthesis IV | 6 | 4 | 2 |
| pyruvate fermentation to (S)-acetoin | 3 | 2 | 1 |
| pyruvate fermentation to (R)-acetoin I | 3 | 2 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 2 |
| L-isoleucine biosynthesis III | 7 | 4 | 2 |
| L-isoleucine biosynthesis II | 8 | 6 | 2 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 4 |
| superpathway of (R,R)-butanediol biosynthesis | 5 | 3 | 1 |
| superpathway of 2,3-butanediol biosynthesis | 6 | 2 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 2 |
| superpathway of L-threonine metabolism | 18 | 13 | 2 |