Experiment set2IT072 for Bifidobacterium breve UCC2003

Compare to:

CDM1 without Leucine

Group: nutrient dropout
Media: CDM1 + no Leucine, pH=7
Culturing: Bifido_ML2, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Anthony Shiver on 12/10/2019
Growth plate: CDM1_nutrientdrop F12

Specific Phenotypes

For 8 genes in this experiment

For nutrient dropout no Leucine in Bifidobacterium breve UCC2003

For nutrient dropout no Leucine across organisms

SEED Subsystems

Subsystem #Specific
Branched-Chain Amino Acid Biosynthesis 6
Leucine Biosynthesis 3
Acetoin, butanediol metabolism 1
Coenzyme A Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-leucine biosynthesis 6 6 5
L-valine biosynthesis 4 4 3
3-methylbutanol biosynthesis (engineered) 7 6 5
superpathway of branched chain amino acid biosynthesis 17 17 11
L-isoleucine biosynthesis II 8 6 5
pyruvate fermentation to isobutanol (engineered) 5 4 3
pyruvate fermentation to (R)-acetoin II 2 2 1
L-isoleucine biosynthesis IV 6 4 3
L-isoleucine biosynthesis I (from threonine) 7 7 3
L-isoleucine biosynthesis III 7 4 3
pyruvate fermentation to (S)-acetoin 3 2 1
pyruvate fermentation to (R)-acetoin I 3 2 1
aliphatic glucosinolate biosynthesis, side chain elongation cycle 30 10 10
phosphopantothenate biosynthesis I 4 2 1
phosphopantothenate biosynthesis III (archaea) 4 1 1
superpathway of L-isoleucine biosynthesis I 13 13 3
superpathway of (R,R)-butanediol biosynthesis 5 3 1
superpathway of L-threonine metabolism 18 13 3
superpathway of 2,3-butanediol biosynthesis 6 2 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
superpathway of coenzyme A biosynthesis I (bacteria) 9 4 1
superpathway of coenzyme A biosynthesis II (plants) 10 2 1
2,5-xylenol and 3,5-xylenol degradation 13 1 1