Experiment set2IT067 for Herbaspirillum seropedicae SmR1

LB with Cobalt chloride hexahydrate 0.2 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.2 mM)
Culturing: HerbieS_ML4, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.0 generations
By: Mark on 3/10/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Herbie_SP2 A5

Specific Phenotypes

For 10 genes in this experiment

For stress Cobalt chloride hexahydrate in Herbaspirillum seropedicae SmR1

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Cell Division	1
Campylobacter Iron Metabolism	1
Central meta-cleavage pathway of aromatic compound degradation	1
Cobalt-zinc-cadmium resistance	1
Copper homeostasis: copper tolerance	1
DNA repair, UvrABC system	1
Formate hydrogenase	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate oxidation to CO2	1	1	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	2	1
oxalate degradation VI	4	1	1
oxalate degradation III	5	1	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	2	1
superpathway of C1 compounds oxidation to CO2	12	4	1
purine nucleobases degradation I (anaerobic)	15	6	1
purine nucleobases degradation II (anaerobic)	24	15	1