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Experiment set2IT061 for
Methanococcus maripaludis JJ
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Collected from freezer stock
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS04110
+2.8
8.7
hypothetical protein
compare
MMJJ
_RS03160
+2.7
15.3
winged helix-turn-helix transcriptional regulator
compare
MMJJ
_RS08320
+2.1
4.2
CDP-diacylglycerol--serine O-phosphatidyltransferase
compare
MMJJ
_RS05930
+1.7
3.4
cell division protein SepF
compare
MMJJ
_RS06380
+1.7
6.0
signal recognition particle protein Srp54
compare
MMJJ
_RS07785
+1.5
4.9
fumarate reductase subunit A
compare
MMJJ
_RS00210
+1.4
3.7
DUF530 family protein
compare
MMJJ
_RS07275
+1.3
3.2
formate--phosphoribosylaminoimidazolecarboxamide ligase
compare
MMJJ
_RS06785
+1.3
7.9
RNA ligase partner protein
compare
MMJJ
_RS01475
+1.3
2.5
signal recognition particle-docking protein FtsY
compare
MMJJ
_RS08775
+1.2
9.6
PfkB family carbohydrate kinase
compare
MMJJ
_RS08250
+1.2
5.9
hydrogenase maturation peptidase HycI
compare
MMJJ
_RS08765
+1.2
10.1
glycosyltransferase family 9 protein
compare
MMJJ
_RS03820
+1.2
3.3
hydrogenase formation protein HypD
compare
MMJJ
_RS00430
+1.1
2.6
ribonuclease Z
compare
MMJJ
_RS03615
+1.1
3.3
cobalt-precorrin-8 methylmutase
compare
MMJJ
_RS03995
+1.1
5.5
PHP domain-containing protein
compare
MMJJ
_RS01355
+1.1
3.2
UDP-N-acetyl-D-mannosamine dehydrogenase
compare
MMJJ
_RS00360
+1.1
2.9
adenosylhomocysteinase
compare
MMJJ
_RS05950
+1.1
3.3
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
compare
MMJJ
_RS05610
+1.1
4.7
CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase
compare
MMJJ
_RS05225
+1.1
1.5
Gar1/Naf1 family protein
compare
MMJJ
_RS08615
+1.1
7.4
oligosaccharide repeat unit polymerase family protein
compare
MMJJ
_RS08785
+1.0
7.3
D-sedoheptulose 7-phosphate isomerase
compare
MMJJ
_RS06880
+1.0
1.4
EhaG family protein
compare
MMJJ
_RS03580
+1.0
2.8
TIGR00303 family protein
compare
MMJJ
_RS05260
+1.0
4.6
ATP-grasp domain-containing protein
compare
MMJJ
_RS05780
+1.0
3.0
flagellin
compare
MMJJ
_RS08780
+1.0
5.4
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
compare
MMJJ
_RS06875
+1.0
2.6
hypothetical protein
compare
MMJJ
_RS01750
+1.0
3.6
ferrous iron transport protein A
compare
MMJJ
_RS02275
+1.0
4.1
nucleotidyltransferase domain-containing protein
compare
MMJJ
_RS04790
+1.0
3.3
biotin--[acetyl-CoA-carboxylase] ligase
compare
MMJJ
_RS05710
+0.9
4.5
single-stranded-DNA-specific exonuclease RecJ
compare
MMJJ
_RS06170
+0.9
6.9
hypothetical protein
compare
MMJJ
_RS00570
+0.9
1.6
ribonuclease P
compare
MMJJ
_RS06200
+0.9
2.0
phosphoglycerate dehydrogenase
compare
MMJJ
_RS05765
+0.9
1.7
flagellar protein E
compare
MMJJ
_RS06240
+0.9
2.3
dihydropteroate synthase-like protein
compare
MMJJ
_RS06110
+0.9
2.0
bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC
compare
MMJJ
_RS08095
+0.9
4.1
cobyric acid synthase CobQ
compare
MMJJ
_RS08190
+0.9
3.3
hypothetical protein
compare
MMJJ
_RS07030
+0.9
7.9
hypothetical protein
compare
MMJJ
_RS06175
+0.9
2.7
DUF1743 domain-containing protein
compare
MMJJ
_RS03670
+0.9
3.4
precorrin-2 C(20)-methyltransferase
compare
MMJJ
_RS01360
+0.8
1.6
UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
compare
MMJJ
_RS04150
+0.8
3.1
DUF5379 domain-containing protein
compare
MMJJ
_RS01880
+0.8
1.8
RlmE family RNA methyltransferase
compare
MMJJ
_RS05295
+0.8
5.9
cobyrinic acid a,c-diamide synthase
compare
MMJJ
_RS08725
+0.8
6.8
flippase
compare
MMJJ
_RS04975
+0.8
1.9
glycosyltransferase family 4 protein
compare
MMJJ
_RS05420
+0.8
2.3
DNA-directed DNA polymerase II small subunit
compare
MMJJ
_RS06490
+0.8
2.9
methanogenesis marker 2 protein
compare
MMJJ
_RS01415
+0.8
2.1
beta-CASP ribonuclease aCPSF1
compare
MMJJ
_RS07380
+0.8
5.1
sulfide-dependent adenosine diphosphate thiazole synthase
compare
MMJJ
_RS03430
+0.8
2.3
winged helix-turn-helix domain-containing protein
compare
MMJJ
_RS05820
+0.8
4.4
DUF2304 domain-containing protein
compare
MMJJ
_RS04615
+0.8
2.8
methanogenesis marker 3 protein
compare
MMJJ
_RS04200
+0.8
4.8
triphosphoribosyl-dephospho-CoA synthase
compare
MMJJ
_RS03060
+0.8
2.2
methanogenesis marker 7 protein
compare
MMJJ
_RS03840
+0.8
3.2
DHH family phosphoesterase
compare
MMJJ
_RS00215
+0.8
2.1
CCA tRNA nucleotidyltransferase
compare
MMJJ
_RS03125
+0.8
3.2
GMC oxidoreductase
compare
MMJJ
_RS01960
+0.8
4.9
glycosyltransferase family 2 protein
compare
MMJJ
_RS06215
+0.8
3.4
agmatinase
compare
MMJJ
_RS07705
+0.8
2.3
GTP-binding protein EngB
compare
MMJJ
_RS06390
+0.8
2.0
NADPH-dependent F420 reductase
compare
MMJJ
_RS05445
+0.8
1.7
2-oxoacid:acceptor oxidoreductase subunit alpha
compare
MMJJ
_RS03785
+0.8
2.1
hypothetical protein
compare
MMJJ
_RS02045
+0.8
2.9
molybdenum cofactor guanylyltransferase
compare
MMJJ
_RS08155
+0.7
3.6
cobalt-precorrin-5B (C(1))-methyltransferase CbiD
compare
MMJJ
_RS01810
+0.7
1.2
methyl coenzyme M reductase system, component A2
compare
MMJJ
_RS04675
+0.7
4.4
thiamine pyrophosphate-binding protein
compare
MMJJ
_RS03875
+0.7
2.9
CBS domain-containing protein
compare
MMJJ
_RS04650
+0.7
3.1
nitrogenase iron protein
compare
MMJJ
_RS04505
+0.7
3.6
CDC48 family AAA ATPase
compare
MMJJ
_RS05870
+0.7
3.0
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMJJ
_RS08715
+0.7
4.9
NAD-dependent epimerase/dehydratase family protein
compare
MMJJ
_RS09035
+0.7
3.3
OB-fold nucleic acid binding domain-containing protein
compare
MMJJ
_RS00265
+0.7
4.0
TIGR02253 family HAD-type hydrolase
compare
MMJJ
_RS09375
+0.7
1.2
hypothetical protein
compare
MMJJ
_RS00195
+0.7
2.8
precorrin-3B C(17)-methyltransferase
compare
MMJJ
_RS03585
+0.7
0.8
methanogenesis marker 6 protein
compare
MMJJ
_RS05065
+0.7
3.1
(4-{4-[2-(gamma-L- glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase
compare
MMJJ
_RS03660
+0.7
2.1
DUF447 family protein
compare
MMJJ
_RS03310
+0.7
2.8
hypothetical protein
compare
MMJJ
_RS02090
+0.7
3.8
tyramine--L-glutamate ligase
compare
MMJJ
_RS08325
+0.7
1.6
glycosyltransferase family 2 protein
compare
MMJJ
_RS05935
+0.7
1.9
Era-like GTP-binding protein
compare
MMJJ
_RS08770
+0.7
3.8
HAD family hydrolase
compare
MMJJ
_RS00425
+0.7
2.9
hypothetical protein
compare
MMJJ
_RS03965
+0.7
1.3
DNA-directed RNA polymerase subunit L
compare
MMJJ
_RS06805
+0.7
2.5
monovalent cation/H+ antiporter subunit E
compare
MMJJ
_RS04155
+0.7
2.9
hypothetical protein
compare
MMJJ
_RS04655
+0.6
2.4
FAD-dependent oxidoreductase
compare
MMJJ
_RS06705
+0.6
2.9
bifunctional NADP phosphatase/NAD kinase
compare
MMJJ
_RS05655
+0.6
3.3
HisA/HisF family protein
compare
MMJJ
_RS08710
+0.6
4.8
UTP--glucose-1-phosphate uridylyltransferase GalU
compare
MMJJ
_RS00275
+0.6
1.8
coenzyme F420-0:L-glutamate ligase
compare
MMJJ
_RS07155
+0.6
4.4
[5-(aminomethyl)furan-3-yl]methyl phosphate kinase
compare
MMJJ
_RS07485
+0.6
3.7
DedA family protein
compare
MMJJ
_RS03765
+0.6
3.6
hypothetical protein
compare
MMJJ
_RS06820
+0.6
3.6
CBS domain-containing protein
compare
MMJJ
_RS02200
+0.6
4.0
chorismate pyruvate-lyase family protein
compare
MMJJ
_RS03225
+0.6
1.7
4Fe-4S binding protein
compare
MMJJ
_RS06040
+0.6
2.1
NADH-quinone oxidoreductase subunit H
compare
MMJJ
_RS00560
+0.6
1.9
homoisocitrate dehydrogenase
compare
MMJJ
_RS06220
+0.6
2.9
polyamine aminopropyltransferase
compare
MMJJ
_RS00270
+0.6
1.5
adenosylcobinamide-GDP ribazoletransferase
compare
MMJJ
_RS05995
+0.6
1.5
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS04375
+0.6
2.2
hypothetical protein
compare
MMJJ
_RS07840
+0.6
1.4
Glu-tRNA(Gln) amidotransferase subunit GatD
compare
MMJJ
_RS03760
+0.6
1.5
hydrogenase maturation nickel metallochaperone HypA
compare
MMJJ
_RS09100
+0.6
3.9
COG1361 S-layer family protein
compare
MMJJ
_RS01935
+0.6
1.7
O-phosphoseryl-tRNA(Sec) selenium transferase
compare
MMJJ
_RS00180
+0.6
3.5
DNA topoisomerase I
compare
MMJJ
_RS06700
+0.6
2.6
L-seryl-tRNA(Sec) kinase
compare
MMJJ
_RS09175
+0.6
1.0
anthranilate synthase component I
compare
MMJJ
_RS05810
+0.6
2.8
precorrin-4 C(11)-methyltransferase
compare
MMJJ
_RS04875
+0.6
1.1
tRNA-Lys
compare
MMJJ
_RS08340
+0.5
2.8
archaeoflavoprotein AfpA
compare
MMJJ
_RS04480
+0.5
2.8
hypothetical protein
compare
MMJJ
_RS06250
+0.5
1.3
nicotinamide-nucleotide adenylyltransferase
compare
MMJJ
_RS04575
+0.5
3.9
DUF116 domain-containing protein
compare
MMJJ
_RS06930
+0.5
1.3
type II methionyl aminopeptidase
compare
MMJJ
_RS09190
+0.5
0.9
tryptophan synthase subunit beta
compare
MMJJ
_RS04765
+0.5
3.6
metal-dependent hydrolase
compare
MMJJ
_RS07190
+0.5
2.0
RtcB family protein
compare
MMJJ
_RS03635
+0.5
1.7
methionine--tRNA ligase
compare
MMJJ
_RS00205
+0.5
2.0
adenosylcobinamide-phosphate synthase CbiB
compare
MMJJ
_RS06760
+0.5
1.8
Ni-sirohydrochlorin a,c-diamide synthase
compare
MMJJ
_RS07630
+0.5
4.0
hypothetical protein
compare
MMJJ
_RS03005
+0.5
1.1
Ni-sirohydrochlorin a,c-diamide reductive cyclase catalytic subunit
compare
MMJJ
_RS06725
+0.5
3.8
MogA/MoaB family molybdenum cofactor biosynthesis protein
compare
MMJJ
_RS08330
+0.5
1.2
SufD family Fe-S cluster assembly protein
compare
MMJJ
_RS01690
+0.5
1.5
methanogenesis marker 12 protein
compare
MMJJ
_RS08925
+0.5
3.8
winged helix-turn-helix transcriptional regulator
compare
MMJJ
_RS03150
+0.5
2.8
2-phospho-L-lactate transferase
compare
MMJJ
_RS03655
+0.5
1.8
replication factor C large subunit
compare
MMJJ
_RS00355
+0.5
1.7
ribonuclease P protein component 4
compare
MMJJ
_RS09295
+0.5
0.7
hypothetical protein
compare
MMJJ
_RS05620
+0.5
2.0
CoB--CoM heterodisulfide reductase iron-sulfur subunit A family protein
compare
MMJJ
_RS08720
+0.5
4.0
UDP-glucose/GDP-mannose dehydrogenase family protein
compare
MMJJ
_RS08130
+0.5
0.6
translation initiation factor IF-2 subunit gamma
compare
MMJJ
_RS08230
+0.5
1.7
ribose-5-phosphate isomerase RpiA
compare
MMJJ
_RS04520
+0.5
2.2
coenzyme F430 synthase
compare
MMJJ
_RS07035
+0.5
1.0
glycosyl transferase
compare
MMJJ
_RS07345
+0.4
2.4
homocysteine biosynthesis protein
compare
MMJJ
_RS03185
+0.4
1.3
alanine--tRNA ligase
compare
MMJJ
_RS00285
+0.4
3.7
UPF0254 family protein
compare
MMJJ
_RS01470
+0.4
1.9
Hsp20/alpha crystallin family protein
compare
MMJJ
_RS02935
+0.4
0.7
DNA-directed RNA polymerase subunit E''
compare
MMJJ
_RS03155
+0.4
3.0
hypothetical protein
compare
MMJJ
_RS01395
+0.4
1.8
DUF1894 domain-containing protein
compare
MMJJ
_RS01210
+0.4
1.0
hypothetical protein
compare
MMJJ
_RS05650
+0.4
1.3
formylmethanofuran dehydrogenase subunit B
compare
MMJJ
_RS04965
+0.4
1.8
RNA polymerase Rpb4 family protein
compare
MMJJ
_RS05645
+0.4
1.2
4Fe-4S binding protein
compare
MMJJ
_RS03270
+0.4
0.7
ribosome biogenesis/translation initiation ATPase RLI
compare
MMJJ
_RS08020
+0.4
2.7
nucleotidyltransferase domain-containing protein
compare
MMJJ
_RS06155
+0.4
2.3
phosphatidylglycerophosphatase A
compare
MMJJ
_RS07405
+0.4
1.8
histone family protein
compare
MMJJ
_RS03055
+0.4
1.6
hypothetical protein
compare
MMJJ
_RS06185
+0.4
1.9
cobalamin biosynthesis protein
compare
MMJJ
_RS07040
+0.4
0.9
preprotein translocase subunit SecY
compare
MMJJ
_RS01785
+0.4
0.8
50S ribosomal protein L14e
compare
MMJJ
_RS03295
+0.4
3.5
PHP domain-containing protein
compare
MMJJ
_RS07370
+0.4
1.7
tRNA 4-thiouridine(8) synthase ThiI
compare
MMJJ
_RS08175
+0.4
2.7
winged helix-turn-helix transcriptional regulator
compare
MMJJ
_RS06860
+0.4
2.1
hypothetical protein
compare
MMJJ
_RS08850
+0.4
0.9
succinate dehydrogenase/fumarate reductase iron-sulfur subunit
compare
MMJJ
_RS04755
+0.4
3.0
5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB
compare
MMJJ
_RS01565
+0.4
2.4
TIGR00267 family protein
compare
MMJJ
_RS03650
+0.4
2.0
class I SAM-dependent methyltransferase family protein
compare
MMJJ
_RS04985
+0.4
1.0
glutamyl-tRNA reductase
compare
MMJJ
_RS01940
+0.4
2.7
hypothetical protein
compare
MMJJ
_RS08620
+0.4
1.4
DUF373 family protein
compare
MMJJ
_RS01755
+0.4
0.7
ferrous iron transport protein B
compare
MMJJ
_RS05490
+0.4
1.0
imidazole glycerol phosphate synthase subunit HisF
compare
MMJJ
_RS06975
+0.4
1.4
protein translocase SEC61 complex subunit gamma
compare
MMJJ
_RS04980
+0.4
1.9
bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase
compare
MMJJ
_RS08055
+0.4
2.2
methanogenesis marker 14 protein
compare
MMJJ
_RS00415
+0.4
2.2
DedA family protein
compare
MMJJ
_RS07960
+0.4
1.3
quinolinate synthase NadA
compare
MMJJ
_RS07280
+0.4
2.4
phosphomannomutase/phosphoglucomutase
compare
MMJJ
_RS07350
+0.4
1.3
4Fe-4S binding protein
compare
MMJJ
_RS07725
+0.4
1.5
DNA primase DnaG
compare
MMJJ
_RS03895
+0.4
1.5
hydrogenase expression/formation protein HypE
compare
MMJJ
_RS06665
+0.4
1.8
DUF3236 domain-containing protein
compare
MMJJ
_RS00370
+0.4
0.6
asparagine synthase (glutamine-hydrolyzing)
compare
MMJJ
_RS07980
+0.4
2.8
biotin synthase BioB
compare
MMJJ
_RS03490
+0.4
0.7
ribonuclease VapC
compare
MMJJ
_RS03350
+0.4
0.9
hypothetical protein
compare
MMJJ
_RS03845
+0.4
1.6
translation initiation factor IF-2
compare
MMJJ
_RS04750
+0.4
2.7
5,10-methenyltetrahydromethanopterin hydrogenase
compare
MMJJ
_RS04760
+0.4
3.1
5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC
compare
MMJJ
_RS04475
+0.4
1.2
3,4-dihydroxy-2-butanone-4-phosphate synthase
compare
MMJJ
_RS07775
+0.4
2.1
fluoride efflux transporter CrcB
compare
MMJJ
_RS02120
+0.4
2.0
DJ-1/PfpI family protein
compare
MMJJ
_RS06225
+0.3
0.8
adenosylmethionine decarboxylase
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in freezer experiments