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Experiment set2IT058 for
Methanococcus maripaludis JJ
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
200 most important genes:
gene
name
fitness
t score
description
MMJJ
_RS00235
-6.1
-23.0
hypothetical protein
compare
MMJJ
_RS06260
-6.0
-5.8
6-carboxyhexanoate--CoA ligase
compare
MMJJ
_RS03055
-5.9
-3.6
hypothetical protein
compare
MMJJ
_RS03630
-5.8
-15.6
AMP phosphorylase
compare
MMJJ
_RS00645
-5.5
-9.2
adenosylmethionine--8-amino-7-oxononanoate transaminase
compare
MMJJ
_RS08870
-5.5
-1.5
2-isopropylmalate synthase
compare
MMJJ
_RS05155
-5.4
-5.3
5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH
compare
MMJJ
_RS00580
-5.4
-2.5
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofG
compare
MMJJ
_RS03620
-5.4
-3.7
DUF2097 domain-containing protein
compare
MMJJ
_RS07360
-5.3
-2.5
TIGR00269 family protein
compare
MMJJ
_RS03800
-5.3
-12.2
aldolase
compare
MMJJ
_RS06600
-5.2
-3.2
DUF555 domain-containing protein
compare
MMJJ
_RS03170
-5.2
-11.6
DUF5612 domain-containing protein
compare
MMJJ
_RS04200
-5.1
-5.0
triphosphoribosyl-dephospho-CoA synthase
compare
MMJJ
_RS06375
-5.1
-3.5
UbiX family flavin prenyltransferase
compare
MMJJ
_RS08080
-5.1
-10.3
TIGR00300 family protein
compare
MMJJ
_RS07720
-5.0
-2.7
UPF0058 family protein
compare
MMJJ
_RS06270
-5.0
-6.0
dethiobiotin synthase
compare
MMJJ
_RS02985
-4.9
-16.3
MBL fold metallo-hydrolase
compare
MMJJ
_RS04145
-4.9
-9.9
glutamate-1-semialdehyde 2,1-aminomutase
compare
MMJJ
_RS00560
-4.9
-3.3
homoisocitrate dehydrogenase
compare
MMJJ
_RS08625
-4.8
-3.3
magnesium/cobalt transporter CorA
compare
MMJJ
_RS02200
-4.8
-8.1
chorismate pyruvate-lyase family protein
compare
MMJJ
_RS08155
-4.8
-3.3
cobalt-precorrin-5B (C(1))-methyltransferase CbiD
compare
MMJJ
_RS06265
-4.7
-15.5
8-amino-7-oxononanoate synthase
compare
MMJJ
_RS01935
-4.6
-3.2
O-phosphoseryl-tRNA(Sec) selenium transferase
compare
MMJJ
_RS00600
-4.6
-3.0
hydroxymethylbilane synthase
compare
MMJJ
_RS07980
-4.6
-14.3
biotin synthase BioB
compare
MMJJ
_RS05535
-4.6
-15.3
hypothetical protein
compare
MMJJ
_RS06695
-4.6
-3.1
amidohydrolase
compare
MMJJ
_RS02045
-4.5
-3.6
molybdenum cofactor guanylyltransferase
compare
MMJJ
_RS04050
-4.5
-2.1
dihydroneopterin aldolase family protein
compare
MMJJ
_RS03920
-4.5
-15.7
nickel pincer cofactor biosynthesis protein LarC
compare
MMJJ
_RS07160
-4.5
-22.3
M20 family metallo-hydrolase
compare
MMJJ
_RS06510
-4.5
-3.0
LL-diaminopimelate aminotransferase
compare
MMJJ
_RS08535
-4.5
-3.1
NOL1/NOP2/sun family putative RNA methylase
compare
MMJJ
_RS03110
-4.5
-3.1
tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase
compare
MMJJ
_RS04475
-4.4
-5.6
3,4-dihydroxy-2-butanone-4-phosphate synthase
compare
MMJJ
_RS00370
-4.4
-2.4
asparagine synthase (glutamine-hydrolyzing)
compare
MMJJ
_RS04800
-4.4
-6.7
2-phospho-L-lactate guanylyltransferase
compare
MMJJ
_RS00450
-4.4
-2.3
DUF126 domain-containing protein
compare
MMJJ
_RS06725
-4.4
-13.2
MogA/MoaB family molybdenum cofactor biosynthesis protein
compare
MMJJ
_RS06040
-4.4
-3.0
NADH-quinone oxidoreductase subunit H
compare
MMJJ
_RS03230
-4.4
-5.2
NAD(P)/FAD-dependent oxidoreductase
compare
MMJJ
_RS01880
-4.4
-3.0
RlmE family RNA methyltransferase
compare
MMJJ
_RS02920
-4.4
-2.3
30S ribosomal protein S27ae
compare
MMJJ
_RS03760
-4.4
-3.0
hydrogenase maturation nickel metallochaperone HypA
compare
MMJJ
_RS00205
-4.3
-5.9
adenosylcobinamide-phosphate synthase CbiB
compare
MMJJ
_RS04730
-4.3
-3.0
tyrosine decarboxylase MfnA
compare
MMJJ
_RS04685
-4.3
-9.2
carbamoyltransferase HypF
compare
MMJJ
_RS05770
-4.3
-3.4
flagellar protein D
compare
MMJJ
_RS06155
-4.3
-5.1
phosphatidylglycerophosphatase A
compare
MMJJ
_RS05590
-4.3
-2.3
homoserine dehydrogenase
compare
MMJJ
_RS03815
-4.3
-2.3
nascent polypeptide-associated complex protein
compare
MMJJ
_RS07950
-4.3
-4.1
4Fe-4S binding protein
compare
MMJJ
_RS02275
-4.3
-5.0
nucleotidyltransferase domain-containing protein
compare
MMJJ
_RS03065
-4.2
-13.9
CBS domain-containing protein
compare
MMJJ
_RS06930
-4.2
-2.8
type II methionyl aminopeptidase
compare
MMJJ
_RS01350
-4.2
-7.0
sodium:proton antiporter
compare
MMJJ
_RS05445
-4.2
-2.3
2-oxoacid:acceptor oxidoreductase subunit alpha
compare
MMJJ
_RS06625
-4.2
-2.3
pyruvate synthase subunit PorB
compare
MMJJ
_RS00270
-4.2
-2.8
adenosylcobinamide-GDP ribazoletransferase
compare
MMJJ
_RS03200
-4.2
-2.5
uroporphyrinogen-III synthase
compare
MMJJ
_RS08035
-4.1
-9.5
cobalt-precorrin-7 (C(5))-methyltransferase
compare
MMJJ
_RS08660
-4.1
-2.8
acetylornithine transaminase
compare
MMJJ
_RS03615
-4.1
-2.8
cobalt-precorrin-8 methylmutase
compare
MMJJ
_RS07350
-4.1
-3.9
4Fe-4S binding protein
compare
MMJJ
_RS01600
-4.1
-2.8
radical SAM protein
compare
MMJJ
_RS07470
-4.1
-2.1
50S ribosome-binding GTPase
compare
MMJJ
_RS03005
-4.0
-2.8
Ni-sirohydrochlorin a,c-diamide reductive cyclase catalytic subunit
compare
MMJJ
_RS04485
-4.0
-10.6
riboflavin synthase
compare
MMJJ
_RS07430
-4.0
-4.2
ribonuclease VapC
compare
MMJJ
_RS00275
-4.0
-2.4
coenzyme F420-0:L-glutamate ligase
compare
MMJJ
_RS08925
-4.0
-15.6
winged helix-turn-helix transcriptional regulator
compare
MMJJ
_RS04995
-4.0
-6.0
Tfx family DNA-binding protein
compare
MMJJ
_RS06805
-4.0
-3.6
monovalent cation/H+ antiporter subunit E
compare
MMJJ
_RS01810
-3.9
-2.4
methyl coenzyme M reductase system, component A2
compare
MMJJ
_RS03190
-3.9
-2.7
phosphopyruvate hydratase
compare
MMJJ
_RS03580
-3.9
-2.7
TIGR00303 family protein
compare
MMJJ
_RS06180
-3.9
-2.7
tryptophan--tRNA ligase
compare
MMJJ
_RS05995
-3.9
-2.3
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS00570
-3.9
-2.2
ribonuclease P
compare
MMJJ
_RS01415
-3.9
-2.6
beta-CASP ribonuclease aCPSF1
compare
MMJJ
_RS01750
-3.9
-3.4
ferrous iron transport protein A
compare
MMJJ
_RS03670
-3.9
-3.7
precorrin-2 C(20)-methyltransferase
compare
MMJJ
_RS01755
-3.8
-2.6
ferrous iron transport protein B
compare
MMJJ
_RS08855
-3.8
-5.4
cyclic pyranopterin monophosphate synthase MoaC
compare
MMJJ
_RS04700
-3.8
-7.3
deoxyhypusine synthase
compare
MMJJ
_RS06220
-3.8
-4.5
polyamine aminopropyltransferase
compare
MMJJ
_RS06175
-3.8
-2.3
DUF1743 domain-containing protein
compare
MMJJ
_RS03125
-3.8
-4.5
GMC oxidoreductase
compare
MMJJ
_RS04650
-3.8
-5.8
nitrogenase iron protein
compare
MMJJ
_RS04975
-3.8
-2.3
glycosyltransferase family 4 protein
compare
MMJJ
_RS03785
-3.8
-2.6
hypothetical protein
compare
MMJJ
_RS06750
-3.8
-3.0
homoaconitase large subunit
compare
MMJJ
_RS04680
-3.8
-13.0
topoisomerase DNA-binding C4 zinc finger domain-containing protein
compare
MMJJ
_RS05720
-3.8
-2.6
glutamine--fructose-6-phosphate transaminase (isomerizing)
compare
MMJJ
_RS03840
-3.8
-2.6
DHH family phosphoesterase
compare
MMJJ
_RS03660
-3.8
-3.6
DUF447 family protein
compare
MMJJ
_RS08655
-3.8
-12.0
phospholipase D-like domain-containing protein
compare
MMJJ
_RS05650
-3.7
-3.6
formylmethanofuran dehydrogenase subunit B
compare
MMJJ
_RS09135
-3.7
-11.6
archaeosine biosynthesis radical SAM protein RaSEA
compare
MMJJ
_RS05620
-3.7
-2.6
CoB--CoM heterodisulfide reductase iron-sulfur subunit A family protein
compare
MMJJ
_RS05575
-3.7
-5.0
2-amino-5-formylamino-6-ribosylaminopyrimidin- 4(3H)-one 5'-monophosphate deformylase
compare
MMJJ
_RS00355
-3.7
-3.6
ribonuclease P protein component 4
compare
MMJJ
_RS04155
-3.7
-7.4
hypothetical protein
compare
MMJJ
_RS03060
-3.7
-3.5
methanogenesis marker 7 protein
compare
MMJJ
_RS03865
-3.7
-1.6
phosphoribosyl-AMP cyclohydrolase
compare
MMJJ
_RS05065
-3.7
-5.2
(4-{4-[2-(gamma-L- glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase
compare
MMJJ
_RS06700
-3.6
-6.0
L-seryl-tRNA(Sec) kinase
compare
MMJJ
_RS03270
-3.6
-2.5
ribosome biogenesis/translation initiation ATPase RLI
compare
MMJJ
_RS07945
-3.6
-3.5
4Fe-4S binding protein
compare
MMJJ
_RS06715
-3.6
-9.6
lactaldehyde dehydrogenase
compare
MMJJ
_RS03895
-3.6
-4.5
hydrogenase expression/formation protein HypE
compare
MMJJ
_RS08795
-3.6
-2.5
phosphoglucosamine mutase
compare
MMJJ
_RS03225
-3.6
-3.3
4Fe-4S binding protein
compare
MMJJ
_RS06840
-3.6
-2.5
nickel-dependent hydrogenase large subunit
compare
MMJJ
_RS02090
-3.6
-6.4
tyramine--L-glutamate ligase
compare
MMJJ
_RS04150
-3.6
-4.0
DUF5379 domain-containing protein
compare
MMJJ
_RS06875
-3.6
-2.1
hypothetical protein
compare
MMJJ
_RS07455
-3.6
-7.5
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS07375
-3.5
-12.4
tRNA 2-thiocytidine biosynthesis TtcA family protein
compare
MMJJ
_RS06490
-3.5
-2.4
methanogenesis marker 2 protein
compare
MMJJ
_RS03650
-3.5
-8.2
class I SAM-dependent methyltransferase family protein
compare
MMJJ
_RS08020
-3.5
-10.5
nucleotidyltransferase domain-containing protein
compare
MMJJ
_RS08095
-3.5
-4.1
cobyric acid synthase CobQ
compare
MMJJ
_RS06010
-3.5
-7.4
hypothetical protein
compare
MMJJ
_RS05950
-3.5
-3.4
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
compare
MMJJ
_RS06860
-3.5
-4.6
hypothetical protein
compare
MMJJ
_RS05610
-3.5
-4.1
CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase
compare
MMJJ
_RS00210
-3.5
-2.1
DUF530 family protein
compare
MMJJ
_RS08840
-3.5
-4.1
calcium/sodium antiporter
compare
MMJJ
_RS08330
-3.5
-3.3
SufD family Fe-S cluster assembly protein
compare
MMJJ
_RS04675
-3.5
-6.9
thiamine pyrophosphate-binding protein
compare
MMJJ
_RS07190
-3.5
-4.1
RtcB family protein
compare
MMJJ
_RS04970
-3.5
-2.4
DUF655 domain-containing protein
compare
MMJJ
_RS05490
-3.4
-2.3
imidazole glycerol phosphate synthase subunit HisF
compare
MMJJ
_RS08010
-3.4
-8.0
phosphoadenosine phosphosulfate reductase family protein
compare
MMJJ
_RS05070
-3.4
-2.7
DNA primase catalytic subunit PriS
compare
MMJJ
_RS07245
-3.4
-6.5
amidohydrolase family protein
compare
MMJJ
_RS08320
-3.4
-2.1
CDP-diacylglycerol--serine O-phosphatidyltransferase
compare
MMJJ
_RS07170
-3.4
-3.3
pyridoxal phosphate-dependent aminotransferase
compare
MMJJ
_RS03150
-3.3
-7.6
2-phospho-L-lactate transferase
compare
MMJJ
_RS01360
-3.3
-2.2
UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
compare
MMJJ
_RS06240
-3.3
-2.8
dihydropteroate synthase-like protein
compare
MMJJ
_RS04595
-3.3
-5.9
hypothetical protein
compare
MMJJ
_RS03310
-3.3
-3.9
hypothetical protein
compare
MMJJ
_RS03820
-3.3
-2.3
hydrogenase formation protein HypD
compare
MMJJ
_RS08405
-3.3
-13.6
nickel-dependent hydrogenase large subunit
compare
MMJJ
_RS06250
-3.3
-2.2
nicotinamide-nucleotide adenylyltransferase
compare
MMJJ
_RS09375
-3.2
-2.0
hypothetical protein
compare
MMJJ
_RS06215
-3.2
-4.9
agmatinase
compare
MMJJ
_RS00125
-3.2
-3.1
KEOPS complex subunit Cgi121
compare
MMJJ
_RS04980
-3.2
-5.2
bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase
compare
MMJJ
_RS07155
-3.2
-12.1
[5-(aminomethyl)furan-3-yl]methyl phosphate kinase
compare
MMJJ
_RS05655
-3.2
-6.8
HisA/HisF family protein
compare
MMJJ
_RS06705
-3.2
-3.7
bifunctional NADP phosphatase/NAD kinase
compare
MMJJ
_RS08055
-3.1
-8.6
methanogenesis marker 14 protein
compare
MMJJ
_RS00935
-3.1
-10.3
nickel pincer cofactor biosynthesis protein LarB
compare
MMJJ
_RS03965
-3.1
-2.2
DNA-directed RNA polymerase subunit L
compare
MMJJ
_RS02035
-3.1
-7.4
4-hydroxy-tetrahydrodipicolinate synthase
compare
MMJJ
_RS05415
-3.1
-2.5
hypothetical protein
compare
MMJJ
_RS00425
-3.1
-3.0
hypothetical protein
compare
MMJJ
_RS06255
-3.1
-7.1
tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3
compare
MMJJ
_RS05300
-3.1
-18.3
potassium channel protein
compare
MMJJ
_RS01200
-3.1
-2.9
PRC-barrel domain-containing protein
compare
MMJJ
_RS08000
-3.1
-12.5
HesA/MoeB/ThiF family protein
compare
MMJJ
_RS05935
-3.1
-2.5
Era-like GTP-binding protein
compare
MMJJ
_RS06400
-3.1
-6.0
FumA C-terminus/TtdB family hydratase beta subunit
compare
MMJJ
_RS07705
-3.1
-2.9
GTP-binding protein EngB
compare
MMJJ
_RS06015
-3.0
-10.6
Na(+)/H(+) antiporter subunit B
compare
MMJJ
_RS06485
-3.0
-2.9
phosphoglycerate kinase
compare
MMJJ
_RS01870
-3.0
-2.8
methanogenesis marker 15 protein
compare
MMJJ
_RS04790
-3.0
-4.0
biotin--[acetyl-CoA-carboxylase] ligase
compare
MMJJ
_RS09190
-3.0
-2.2
tryptophan synthase subunit beta
compare
MMJJ
_RS05260
-3.0
-5.9
ATP-grasp domain-containing protein
compare
MMJJ
_RS05330
-3.0
-2.0
DNA polymerase II large subunit
compare
MMJJ
_RS03115
-2.9
-5.8
sulfopyruvate decarboxylase subunit alpha
compare
MMJJ
_RS02040
-2.9
-2.8
Asp-tRNA(Asn) amidotransferase subunit GatC
compare
MMJJ
_RS05810
-2.9
-6.1
precorrin-4 C(11)-methyltransferase
compare
MMJJ
_RS07785
-2.9
-2.0
fumarate reductase subunit A
compare
MMJJ
_RS07415
-2.9
-2.7
polyprenyl diphosphate synthase
compare
MMJJ
_RS06000
-2.9
-5.1
cation:proton antiporter subunit C
compare
MMJJ
_RS07975
-2.9
-7.8
ATP-dependent sacrificial sulfur transferase LarE
compare
MMJJ
_RS08500
-2.9
-8.4
L-sulfolactate dehydrogenase
compare
MMJJ
_RS07000
-2.9
-2.1
TrkH family potassium uptake protein
compare
MMJJ
_RS01690
-2.8
-4.1
methanogenesis marker 12 protein
compare
MMJJ
_RS08850
-2.8
-2.8
succinate dehydrogenase/fumarate reductase iron-sulfur subunit
compare
MMJJ
_RS08340
-2.8
-6.6
archaeoflavoprotein AfpA
compare
MMJJ
_RS06760
-2.8
-5.0
Ni-sirohydrochlorin a,c-diamide synthase
compare
MMJJ
_RS07410
-2.8
-4.6
methanogenesis marker protein Mmp4/MtxX
compare
MMJJ
_RS07725
-2.8
-7.7
DNA primase DnaG
compare
MMJJ
_RS05160
-2.8
-5.1
RNA-binding protein
compare
MMJJ
_RS05295
-2.8
-11.9
cobyrinic acid a,c-diamide synthase
compare
MMJJ
_RS07995
-2.8
-5.4
molybdenum cofactor biosynthesis protein MoaE
compare
MMJJ
_RS04520
-2.8
-6.1
coenzyme F430 synthase
compare
MMJJ
_RS02855
-2.8
-8.7
DEAD/DEAH box helicase
compare
MMJJ
_RS03540
-2.7
-7.1
TIGR00289 family protein
compare
MMJJ
_RS06390
-2.7
-1.8
NADPH-dependent F420 reductase
compare
MMJJ
_RS05780
-2.7
-3.6
flagellin
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in rich t2 experiments