Experiment set2IT057 for Sinorhizobium meliloti 1021

Compare to:

LB with D-Cycloserine 0.05 mg/ml

Group: stress
Media: LB + D-Cycloserine (0.05 mg/ml)
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.3 generations
By: Mark on 6/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Smeli_SP5 C1

Specific Phenotypes

For 14 genes in this experiment

For stress D-Cycloserine in Sinorhizobium meliloti 1021

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem #Specific
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 2
Choline and Betaine Uptake and Betaine Biosynthesis 1
Peptidoglycan Biosynthesis 1
Phenylalanine and Tyrosine Branches from Chorismate 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Threonine and Homoserine Biosynthesis 1
Two cell division clusters relating to chromosome partitioning 1
YcfH 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-serine degradation 3 3 3
L-aspartate degradation I 1 1 1
L-aspartate biosynthesis 1 1 1
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
L-tryptophan degradation II (via pyruvate) 3 2 2
D-serine degradation 3 2 2
L-cysteine degradation II 3 2 2
glycine betaine degradation I 8 7 5
malate/L-aspartate shuttle pathway 2 2 1
L-phenylalanine biosynthesis III (cytosolic, plants) 2 2 1
pseudouridine degradation 2 1 1
atromentin biosynthesis 2 1 1
L-tyrosine degradation II 2 1 1
L-glutamate degradation II 2 1 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
glycine betaine degradation III 7 4 3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
L-tyrosine biosynthesis I 3 3 1
L-phenylalanine biosynthesis I 3 3 1
glycine degradation 3 3 1
L-methionine biosynthesis II 6 4 2
L-phenylalanine degradation II (anaerobic) 3 2 1
L-arginine degradation I (arginase pathway) 3 2 1
L-proline degradation I 3 2 1
(R)-cysteate degradation 3 1 1
sulfolactate degradation III 3 1 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
L-asparagine degradation III (mammalian) 3 1 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
L-tyrosine biosynthesis III 4 3 1
L-mimosine degradation 8 4 2
L-phenylalanine biosynthesis II 4 2 1
L-tyrosine biosynthesis II 4 2 1
creatinine degradation I 4 2 1
L-phenylalanine degradation III 4 2 1
L-tyrosine degradation III 4 2 1
glutathione-mediated detoxification I 8 3 2
superpathway of L-aspartate and L-asparagine biosynthesis 4 1 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
ethene biosynthesis II (microbes) 4 1 1
L-tyrosine degradation I 5 5 1
trans-4-hydroxy-L-proline degradation I 5 3 1
superpathway of plastoquinol biosynthesis 5 2 1
superpathway of L-phenylalanine and L-tyrosine biosynthesis 5 2 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
creatinine degradation II 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 7 2
TCA cycle VIII (Chlamydia) 6 6 1
superpathway of L-threonine biosynthesis 6 6 1
superpathway of sulfolactate degradation 6 3 1
coenzyme M biosynthesis II 6 1 1
pyridoxal 5'-phosphate biosynthesis I 7 6 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 12 2
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) 8 8 1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) 8 7 1
purine nucleobases degradation II (anaerobic) 24 16 3
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 4 1
L-histidine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-phenylalanine biosynthesis 10 10 1
rosmarinic acid biosynthesis I 10 2 1
(S)-reticuline biosynthesis I 11 3 1
tropane alkaloids biosynthesis 11 1 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 11 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 11 1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage 12 10 1
indole-3-acetate biosynthesis II 12 5 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of rosmarinic acid biosynthesis 14 3 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis 16 4 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
superpathway of anaerobic energy metabolism (invertebrates) 17 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 23 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
superpathway of chorismate metabolism 59 38 2
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1