Experiment set2IT057 for Burkholderia phytofirmans PsJN

LB, pH4

Group: pH
Media: LB, pH=4
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.7 generations
By: Mark on 6/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 9 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	2
Isoprenoid Biosynthesis	1
Molybdenum cofactor biosynthesis	1
Multidrug Resistance Efflux Pumps	1
Respiratory Complex I	1
polyprenyl synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Oxidative phosphorylation
• Terpenoid biosynthesis
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
bis(guanylyl molybdenum cofactor) biosynthesis	2	2	2
guanylyl molybdenum cofactor biosynthesis	1	1	1
bis(guanylyl tungstenpterin) cofactor biosynthesis	1	1	1
NADH to cytochrome bo oxidase electron transfer I	2	2	1
NADH to cytochrome bd oxidase electron transfer I	2	2	1
aerobic respiration III (alternative oxidase pathway)	3	3	1
aerobic respiration I (cytochrome c)	4	3	1
methylerythritol phosphate pathway II	9	8	2
methylerythritol phosphate pathway I	9	7	2
isoprene biosynthesis I	10	7	2
superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)	12	10	2
NAD(P)/NADPH interconversion	6	3	1
Fe(II) oxidation	6	2	1
taxadiene biosynthesis (engineered)	13	10	2
superpathway of ergosterol biosynthesis II	26	11	2