Experiment set2IT054 for Sinorhizobium meliloti 1021

LB with Fusidic acid sodium salt 0.0001 mg/ml

Group: stress
Media: LB + Fusidic acid sodium salt (1e-04 mg/ml)
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.3 generations
By: Mark on 6/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Smeli_SP4 D1

Specific Phenotypes

For 18 genes in this experiment

For stress Fusidic acid sodium salt in Sinorhizobium meliloti 1021

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
dTDP-rhamnose synthesis	2
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Glycogen metabolism	1
Heat shock dnaK gene cluster extended	1
Histidine Degradation	1
MLST	1
Methionine Biosynthesis	1
Multidrug Resistance Efflux Pumps	1
One-carbon metabolism by tetrahydropterines	1
RNA processing and degradation, bacterial	1
Rhamnose containing glycans	1
Serine-glyoxylate cycle	1
ZZ gjo need homes	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Starch and sucrose metabolism
• Ascorbate and aldarate metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Glycosaminoglycan degradation
• Glycerolipid metabolism
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate
• Methane metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of vancomycin group antibiotics
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	2	1
glycerol degradation I	3	2	1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis	9	5	3
glycogen biosynthesis I (from ADP-D-Glucose)	4	4	1
glycerol and glycerophosphodiester degradation	4	3	1
dTDP-6-deoxy-α-D-allose biosynthesis	4	2	1
dTDP-β-D-fucofuranose biosynthesis	4	2	1
dTDP-N-acetylviosamine biosynthesis	4	2	1
dTDP-N-acetylthomosamine biosynthesis	4	2	1
tRNA processing	10	10	2
dTDP-β-L-rhamnose biosynthesis	5	5	1
L-histidine degradation II	5	5	1
glucosylglycerol biosynthesis	5	3	1
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
CDP-6-deoxy-D-gulose biosynthesis	5	3	1
dTDP-3-acetamido-α-D-fucose biosynthesis	5	2	1
dTDP-α-D-mycaminose biosynthesis	5	2	1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis	5	2	1
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-sibirosamine biosynthesis	6	3	1
dTDP-D-desosamine biosynthesis	6	2	1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis	6	2	1
dTDP-β-L-olivose biosynthesis	7	3	1
dTDP-β-L-digitoxose biosynthesis	7	3	1
dTDP-β-L-mycarose biosynthesis	7	2	1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis	23	5	3
dTDP-β-L-megosamine biosynthesis	8	3	1
dTDP-β-L-4-epi-vancosamine biosynthesis	8	3	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	3	1
dTDP-α-D-forosamine biosynthesis	9	3	1
starch biosynthesis	10	6	1
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)	10	4	1
superpathway of enterobacterial common antigen biosynthesis	10	2	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
folate transformations I	13	9	1
streptomycin biosynthesis	18	3	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of erythromycin biosynthesis	19	2	1
superpathway of megalomicin A biosynthesis	22	3	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	1