Experiment set2IT053 for Burkholderia phytofirmans PsJN

LB with Lomefloxacin hydrochloride 0.001 mM

Group: stress
Media: LB + Lomefloxacin hydrochloride (0.001 mM)
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.1 generations
By: Mark on 6/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: BFirm_SP5 B4

Specific Phenotypes

For 6 genes in this experiment

For stress Lomefloxacin hydrochloride in Burkholderia phytofirmans PsJN

For stress Lomefloxacin hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	2
Alginate metabolism	1
DNA-replication	1
Isoprenoid Biosynthesis	1
Mannose Metabolism	1
polyprenyl synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Terpenoid biosynthesis
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-mannose degradation I	2	1	1
D-mannose degradation II	2	1	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	1	1
GDP-mannose biosynthesis	4	4	1
mannitol degradation II	4	2	1
methylerythritol phosphate pathway II	9	8	2
methylerythritol phosphate pathway I	9	7	2
isoprene biosynthesis I	10	7	2
1,5-anhydrofructose degradation	5	2	1
superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)	12	10	2
taxadiene biosynthesis (engineered)	13	10	2
β-(1,4)-mannan degradation	7	2	1
colanic acid building blocks biosynthesis	11	11	1
superpathway of ergosterol biosynthesis II	26	11	2
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	9	1