Experiment set2IT050 for Pseudomonas fluorescens FW300-N2C3

Compare to:

LB with copper (II) chloride dihydrate 2.5 mM

Group: stress
Media: LB + copper (II) chloride dihydrate (2.5 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 894 B7,B8

Specific Phenotypes

For 26 genes in this experiment

For stress copper (II) chloride dihydrate in Pseudomonas fluorescens FW300-N2C3

For stress copper (II) chloride dihydrate across organisms

SEED Subsystems

Subsystem #Specific
Terminal cytochrome O ubiquinol oxidase 4
Terminal cytochrome oxidases 4
Copper homeostasis 3
ABC transporter dipeptide (TC 3.A.1.5.2) 1
ATP-dependent RNA helicases, bacterial 1
Ammonia assimilation 1
Bacterial Chemotaxis 1
Bacterial Cytoskeleton 1
Cobalt-zinc-cadmium resistance 1
Experimental tye 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Heme and Siroheme Biosynthesis 1
Lysine degradation 1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1
Orphan regulatory proteins 1
ZZ gjo need homes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis I 2 2 2
L-glutamine degradation II 1 1 1
L-glutamine degradation I 1 1 1
ammonia assimilation cycle III 3 3 2
NADH to cytochrome bo oxidase electron transfer II 2 2 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 2 1
succinate to cytochrome bo oxidase electron transfer 2 2 1
proline to cytochrome bo oxidase electron transfer 2 2 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
UDP-α-D-glucose biosynthesis 2 2 1
D-lactate to cytochrome bo oxidase electron transfer 2 1 1
pyruvate to cytochrome bo oxidase electron transfer 2 1 1
trehalose degradation V 3 2 1
GDP-α-D-glucose biosynthesis 3 2 1
L-glutamate and L-glutamine biosynthesis 7 6 2
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 4 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 3 1
heme b biosynthesis II (oxygen-independent) 4 3 1
starch degradation V 4 3 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
heme a biosynthesis 4 2 1
starch degradation III 4 2 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
glucose and glucose-1-phosphate degradation 5 4 1
sucrose degradation II (sucrose synthase) 5 4 1
D-galactose degradation I (Leloir pathway) 5 3 1
glucosylglycerol biosynthesis 5 2 1
CDP-6-deoxy-D-gulose biosynthesis 5 1 1
glycogen degradation II 6 5 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 5 1
superpathway of heme b biosynthesis from uroporphyrinogen-III 6 4 1
sucrose biosynthesis II 8 6 1
L-citrulline biosynthesis 8 6 1
glycogen degradation I 8 6 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 3 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
chitin biosynthesis 9 6 1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) 9 3 1
starch biosynthesis 10 5 1
O-antigen building blocks biosynthesis (E. coli) 11 10 1
colanic acid building blocks biosynthesis 11 9 1
superpathway of L-citrulline metabolism 12 8 1
streptomycin biosynthesis 18 2 1
superpathway of anaerobic sucrose degradation 19 15 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 5 1
superpathway of bacteriochlorophyll a biosynthesis 26 6 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 12 1