Experiment set2IT049 for Methanococcus maripaludis JJ

200 most detrimental genes:

gene	name	fitness	t score	description
MMJJ_RS04110		+2.8	7.7	hypothetical protein
MMJJ_RS03160		+2.7	15.3	winged helix-turn-helix transcriptional regulator
MMJJ_RS05930		+2.0	4.8	cell division protein SepF
MMJJ_RS06380		+1.8	5.7	signal recognition particle protein Srp54
MMJJ_RS08320		+1.7	3.4	CDP-diacylglycerol--serine O-phosphatidyltransferase
MMJJ_RS07275		+1.4	4.2	formate--phosphoribosylaminoimidazolecarboxamide ligase
MMJJ_RS06880		+1.4	3.3	EhaG family protein
MMJJ_RS01355		+1.4	4.3	UDP-N-acetyl-D-mannosamine dehydrogenase
MMJJ_RS04050		+1.3	2.6	dihydroneopterin aldolase family protein
MMJJ_RS08615		+1.3	8.6	oligosaccharide repeat unit polymerase family protein
MMJJ_RS08190		+1.2	3.5	hypothetical protein
MMJJ_RS06785		+1.2	7.2	RNA ligase partner protein
MMJJ_RS01415		+1.2	3.4	beta-CASP ribonuclease aCPSF1
MMJJ_RS08785		+1.2	7.6	D-sedoheptulose 7-phosphate isomerase
MMJJ_RS08775		+1.2	9.2	PfkB family carbohydrate kinase
MMJJ_RS08765		+1.2	9.8	glycosyltransferase family 9 protein
MMJJ_RS06200		+1.1	2.4	phosphoglycerate dehydrogenase
MMJJ_RS07785		+1.1	3.5	fumarate reductase subunit A
MMJJ_RS05225		+1.1	1.4	Gar1/Naf1 family protein
MMJJ_RS01475		+1.1	2.0	signal recognition particle-docking protein FtsY
MMJJ_RS04615		+1.1	4.5	methanogenesis marker 3 protein
MMJJ_RS03360		+1.1	2.3	hypothetical protein
MMJJ_RS06625		+1.1	3.4	pyruvate synthase subunit PorB
MMJJ_RS05260		+1.1	5.1	ATP-grasp domain-containing protein
MMJJ_RS00360		+1.0	3.1	adenosylhomocysteinase
MMJJ_RS06240		+1.0	2.5	dihydropteroate synthase-like protein
MMJJ_RS06875		+1.0	3.1	hypothetical protein
MMJJ_RS05610		+1.0	4.2	CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase
MMJJ_RS04790		+1.0	2.8	biotin--[acetyl-CoA-carboxylase] ligase
MMJJ_RS03995		+1.0	4.6	PHP domain-containing protein
MMJJ_RS00195		+1.0	4.1	precorrin-3B C(17)-methyltransferase
MMJJ_RS00275		+1.0	3.1	coenzyme F420-0:L-glutamate ligase
MMJJ_RS08780		+1.0	6.8	D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
MMJJ_RS02275		+1.0	4.0	nucleotidyltransferase domain-containing protein
MMJJ_RS08935		+1.0	2.4	TIGR00288 family NYN domain-containing protein
MMJJ_RS03815		+0.9	1.9	nascent polypeptide-associated complex protein
MMJJ_RS03760		+0.9	3.5	hydrogenase maturation nickel metallochaperone HypA
MMJJ_RS08250		+0.9	4.2	hydrogenase maturation peptidase HycI
MMJJ_RS08325		+0.9	2.3	glycosyltransferase family 2 protein
MMJJ_RS08340		+0.9	4.1	archaeoflavoprotein AfpA
MMJJ_RS03785		+0.9	2.0	hypothetical protein
MMJJ_RS03615		+0.9	2.6	cobalt-precorrin-8 methylmutase
MMJJ_RS07030		+0.9	7.9	hypothetical protein
MMJJ_RS03840		+0.9	3.6	DHH family phosphoesterase
MMJJ_RS00215		+0.9	2.6	CCA tRNA nucleotidyltransferase
MMJJ_RS04505		+0.9	4.7	CDC48 family AAA ATPase
MMJJ_RS00210		+0.9	1.9	DUF530 family protein
MMJJ_RS08725		+0.9	7.0	flippase
MMJJ_RS06170		+0.9	6.2	hypothetical protein
MMJJ_RS03670		+0.9	2.9	precorrin-2 C(20)-methyltransferase
MMJJ_RS05720		+0.8	2.7	glutamine--fructose-6-phosphate transaminase (isomerizing)
MMJJ_RS00270		+0.8	3.0	adenosylcobinamide-GDP ribazoletransferase
MMJJ_RS06215		+0.8	3.3	agmatinase
MMJJ_RS01960		+0.8	5.4	glycosyltransferase family 2 protein
MMJJ_RS08795		+0.8	2.4	phosphoglucosamine mutase
MMJJ_RS06250		+0.8	2.2	nicotinamide-nucleotide adenylyltransferase
MMJJ_RS04200		+0.8	4.8	triphosphoribosyl-dephospho-CoA synthase
MMJJ_RS05810		+0.8	4.6	precorrin-4 C(11)-methyltransferase
MMJJ_RS06040		+0.8	2.8	NADH-quinone oxidoreductase subunit H
MMJJ_RS08715		+0.8	5.2	NAD-dependent epimerase/dehydratase family protein
MMJJ_RS03965		+0.8	2.0	DNA-directed RNA polymerase subunit L
MMJJ_RS04675		+0.8	4.2	thiamine pyrophosphate-binding protein
MMJJ_RS03310		+0.8	2.7	hypothetical protein
MMJJ_RS05870		+0.8	3.8	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
MMJJ_RS01690		+0.8	2.8	methanogenesis marker 12 protein
MMJJ_RS06930		+0.8	2.3	type II methionyl aminopeptidase
MMJJ_RS09175		+0.8	1.3	anthranilate synthase component I
MMJJ_RS07485		+0.8	5.0	DedA family protein
MMJJ_RS03005		+0.8	1.9	Ni-sirohydrochlorin a,c-diamide reductive cyclase catalytic subunit
MMJJ_RS03430		+0.8	2.4	winged helix-turn-helix domain-containing protein
MMJJ_RS02045		+0.7	2.8	molybdenum cofactor guanylyltransferase
MMJJ_RS04520		+0.7	3.7	coenzyme F430 synthase
MMJJ_RS03660		+0.7	2.8	DUF447 family protein
MMJJ_RS08230		+0.7	1.8	ribose-5-phosphate isomerase RpiA
MMJJ_RS07705		+0.7	2.2	GTP-binding protein EngB
MMJJ_RS05380		+0.7	1.6	hypothetical protein
MMJJ_RS01865		+0.7	3.0	peptidyl-tRNA hydrolase Pth2
MMJJ_RS05415		+0.7	1.5	hypothetical protein
MMJJ_RS06185		+0.7	3.1	cobalamin biosynthesis protein
MMJJ_RS05295		+0.7	5.0	cobyrinic acid a,c-diamide synthase
MMJJ_RS03820		+0.7	2.1	hydrogenase formation protein HypD
MMJJ_RS04650		+0.7	3.2	nitrogenase iron protein
MMJJ_RS01810		+0.7	1.1	methyl coenzyme M reductase system, component A2
MMJJ_RS00475		+0.7	1.5	bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
MMJJ_RS07415		+0.7	1.6	polyprenyl diphosphate synthase
MMJJ_RS04720		+0.7	1.0	IMP dehydrogenase
MMJJ_RS04155		+0.7	3.7	hypothetical protein
MMJJ_RS07935		+0.7	1.7	protein fwdD
MMJJ_RS05070		+0.6	1.4	DNA primase catalytic subunit PriS
MMJJ_RS07840		+0.6	1.8	Glu-tRNA(Gln) amidotransferase subunit GatD
MMJJ_RS07380		+0.6	4.1	sulfide-dependent adenosine diphosphate thiazole synthase
MMJJ_RS08620		+0.6	2.9	DUF373 family protein
MMJJ_RS03060		+0.6	2.2	methanogenesis marker 7 protein
MMJJ_RS05820		+0.6	3.7	DUF2304 domain-containing protein
MMJJ_RS05420		+0.6	1.7	DNA-directed DNA polymerase II small subunit
MMJJ_RS07190		+0.6	2.7	RtcB family protein
MMJJ_RS03845		+0.6	1.9	translation initiation factor IF-2
MMJJ_RS00180		+0.6	4.1	DNA topoisomerase I
MMJJ_RS00070		+0.6	2.9	CooT family nickel-binding protein
MMJJ_RS05780		+0.6	2.0	flagellin
MMJJ_RS05950		+0.6	2.4	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
MMJJ_RS04730		+0.6	1.8	tyrosine decarboxylase MfnA
MMJJ_RS05065		+0.6	2.6	(4-{4-[2-(gamma-L- glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase
MMJJ_RS07630		+0.6	5.2	hypothetical protein
MMJJ_RS04655		+0.6	2.7	FAD-dependent oxidoreductase
MMJJ_RS00425		+0.6	2.5	hypothetical protein
MMJJ_RS05710		+0.6	2.3	single-stranded-DNA-specific exonuclease RecJ
MMJJ_RS04970		+0.6	1.7	DUF655 domain-containing protein
MMJJ_RS03580		+0.6	2.1	TIGR00303 family protein
MMJJ_RS08770		+0.6	3.6	HAD family hydrolase
MMJJ_RS08710		+0.6	4.5	UTP--glucose-1-phosphate uridylyltransferase GalU
MMJJ_RS06175		+0.6	1.8	DUF1743 domain-containing protein
MMJJ_RS06705		+0.6	2.6	bifunctional NADP phosphatase/NAD kinase
MMJJ_RS02920		+0.6	1.6	30S ribosomal protein S27ae
MMJJ_RS06220		+0.6	2.3	polyamine aminopropyltransferase
MMJJ_RS00355		+0.6	1.8	ribonuclease P protein component 4
MMJJ_RS01935		+0.6	2.0	O-phosphoseryl-tRNA(Sec) selenium transferase
MMJJ_RS00265		+0.6	2.9	TIGR02253 family HAD-type hydrolase
MMJJ_RS09100		+0.6	4.2	COG1361 S-layer family protein
MMJJ_RS08095		+0.6	2.2	cobyric acid synthase CobQ
MMJJ_RS06805		+0.6	1.4	monovalent cation/H+ antiporter subunit E
MMJJ_RS08535		+0.6	2.1	NOL1/NOP2/sun family putative RNA methylase
MMJJ_RS08175		+0.6	4.0	winged helix-turn-helix transcriptional regulator
MMJJ_RS05935		+0.6	1.4	Era-like GTP-binding protein
MMJJ_RS04765		+0.5	4.3	metal-dependent hydrolase
MMJJ_RS06750		+0.5	1.1	homoaconitase large subunit
MMJJ_RS07950		+0.5	1.5	4Fe-4S binding protein
MMJJ_RS03125		+0.5	2.0	GMC oxidoreductase
MMJJ_RS06225		+0.5	1.5	adenosylmethionine decarboxylase
MMJJ_RS08055		+0.5	3.1	methanogenesis marker 14 protein
MMJJ_RS04980		+0.5	2.5	bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase
MMJJ_RS01750		+0.5	1.6	ferrous iron transport protein A
MMJJ_RS03110		+0.5	1.8	tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase
MMJJ_RS06110		+0.5	0.8	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC
MMJJ_RS00560		+0.5	2.0	homoisocitrate dehydrogenase
MMJJ_RS04750		+0.5	3.5	5,10-methenyltetrahydromethanopterin hydrogenase
MMJJ_RS06840		+0.5	2.2	nickel-dependent hydrogenase large subunit
MMJJ_RS01880		+0.5	1.5	RlmE family RNA methyltransferase
MMJJ_RS02200		+0.5	3.2	chorismate pyruvate-lyase family protein
MMJJ_RS06885		+0.5	1.5	EhaF family protein
MMJJ_RS08335		+0.5	1.2	ABC transporter ATP-binding protein
MMJJ_RS00570		+0.5	1.1	ribonuclease P
MMJJ_RS04375		+0.5	1.4	hypothetical protein
MMJJ_RS04965		+0.5	1.9	RNA polymerase Rpb4 family protein
MMJJ_RS04670		+0.5	2.0	DUF2117 domain-containing protein
MMJJ_RS01600		+0.5	1.6	radical SAM protein
MMJJ_RS07350		+0.5	1.4	4Fe-4S binding protein
MMJJ_RS08720		+0.5	4.0	UDP-glucose/GDP-mannose dehydrogenase family protein
MMJJ_RS06020		+0.5	2.1	hypothetical protein
MMJJ_RS07155		+0.5	3.3	[5-(aminomethyl)furan-3-yl]methyl phosphate kinase
MMJJ_RS00430		+0.5	1.1	ribonuclease Z
MMJJ_RS08020		+0.4	2.7	nucleotidyltransferase domain-containing protein
MMJJ_RS03065		+0.4	3.6	CBS domain-containing protein
MMJJ_RS07000		+0.4	0.7	TrkH family potassium uptake protein
MMJJ_RS03410		+0.4	2.5	hypothetical protein
MMJJ_RS04575		+0.4	3.1	DUF116 domain-containing protein
MMJJ_RS04680		+0.4	3.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein
MMJJ_RS07945		+0.4	1.5	4Fe-4S binding protein
MMJJ_RS07040		+0.4	0.7	preprotein translocase subunit SecY
MMJJ_RS00205		+0.4	1.6	adenosylcobinamide-phosphate synthase CbiB
MMJJ_RS06255		+0.4	2.4	tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3
MMJJ_RS03350		+0.4	0.8	hypothetical protein
MMJJ_RS05445		+0.4	1.0	2-oxoacid:acceptor oxidoreductase subunit alpha
MMJJ_RS09130		+0.4	0.8	NFYB/HAP3 family transcription factor subunit
MMJJ_RS05995		+0.4	1.1	ATP-binding cassette domain-containing protein
MMJJ_RS06760		+0.4	1.5	Ni-sirohydrochlorin a,c-diamide synthase
MMJJ_RS06725		+0.4	3.3	MogA/MoaB family molybdenum cofactor biosynthesis protein
MMJJ_RS02935		+0.4	0.8	DNA-directed RNA polymerase subunit E''
MMJJ_RS01590		+0.4	0.8	adenine phosphoribosyltransferase
MMJJ_RS09380		+0.4	1.4	hypothetical protein
MMJJ_RS09035		+0.4	2.0	OB-fold nucleic acid binding domain-containing protein
MMJJ_RS06485		+0.4	1.3	phosphoglycerate kinase
MMJJ_RS03650		+0.4	2.3	class I SAM-dependent methyltransferase family protein
MMJJ_RS05645		+0.4	1.3	4Fe-4S binding protein
MMJJ_RS04700		+0.4	2.4	deoxyhypusine synthase
MMJJ_RS03895		+0.4	1.6	hydrogenase expression/formation protein HypE
MMJJ_RS04150		+0.4	1.6	DUF5379 domain-containing protein
MMJJ_RS03370		+0.4	1.1	hypothetical protein
MMJJ_RS03155		+0.4	2.6	hypothetical protein
MMJJ_RS01100		+0.4	1.0	helix-turn-helix domain-containing protein
MMJJ_RS06155		+0.4	2.2	phosphatidylglycerophosphatase A
MMJJ_RS07725		+0.4	2.2	DNA primase DnaG
MMJJ_RS01200		+0.4	1.2	PRC-barrel domain-containing protein
MMJJ_RS08025		+0.4	1.3	hypothetical protein
MMJJ_RS01360		+0.4	0.9	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
MMJJ_RS06700		+0.4	1.9	L-seryl-tRNA(Sec) kinase
MMJJ_RS05765		+0.4	0.5	flagellar protein E
MMJJ_RS07280		+0.4	2.6	phosphomannomutase/phosphoglucomutase
MMJJ_RS07455		+0.4	2.1	coenzyme F420-reducing hydrogenase, FrhD protein
MMJJ_RS01565		+0.4	2.6	TIGR00267 family protein
MMJJ_RS01755		+0.4	0.9	ferrous iron transport protein B
MMJJ_RS08730		+0.4	3.2	glycosyltransferase family 4 protein
MMJJ_RS08625		+0.4	2.3	magnesium/cobalt transporter CorA
MMJJ_RS03490		+0.4	1.3	ribonuclease VapC
MMJJ_RS06975		+0.4	1.3	protein translocase SEC61 complex subunit gamma
MMJJ_RS00285		+0.4	2.9	UPF0254 family protein
MMJJ_RS08135		+0.4	1.7	30S ribosomal protein S6e
MMJJ_RS07775		+0.4	1.9	fluoride efflux transporter CrcB
MMJJ_RS02090		+0.4	1.8	tyramine--L-glutamate ligase
MMJJ_RS06820		+0.4	1.6	CBS domain-containing protein

Specific Phenotypes

None in this experiment

For Methanococcus maripaludis JJ in freezer experiments