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Experiment set2IT047 for
Methanococcus maripaludis JJ
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS08725
+2.3
16.9
flippase
compare
MMJJ
_RS00415
+2.2
10.9
DedA family protein
compare
MMJJ
_RS08720
+1.9
15.8
UDP-glucose/GDP-mannose dehydrogenase family protein
compare
MMJJ
_RS04215
+1.7
13.6
DMT family transporter
compare
MMJJ
_RS04720
+1.6
1.7
IMP dehydrogenase
compare
MMJJ
_RS08730
+1.3
9.3
glycosyltransferase family 4 protein
compare
MMJJ
_RS07030
+1.2
10.3
hypothetical protein
compare
MMJJ
_RS08760
+1.2
7.9
glycosyltransferase family 9 protein
compare
MMJJ
_RS08750
+1.2
9.2
glycosyltransferase
compare
MMJJ
_RS08715
+1.2
6.1
NAD-dependent epimerase/dehydratase family protein
compare
MMJJ
_RS00480
+1.1
7.8
flippase
compare
MMJJ
_RS04480
+1.0
2.6
hypothetical protein
compare
MMJJ
_RS06210
+1.0
5.0
hypothetical protein
compare
MMJJ
_RS01210
+0.9
2.3
hypothetical protein
compare
MMJJ
_RS06130
+0.9
3.2
hypothetical protein
compare
MMJJ
_RS08040
+0.8
6.1
NAD(P)/FAD-dependent oxidoreductase
compare
MMJJ
_RS09380
+0.8
1.8
hypothetical protein
compare
MMJJ
_RS08775
+0.8
4.9
PfkB family carbohydrate kinase
compare
MMJJ
_RS03605
+0.8
2.8
TIGR00297 family protein
compare
MMJJ
_RS09265
+0.8
2.3
hypothetical protein
compare
MMJJ
_RS00525
+0.8
1.9
Dabb family protein
compare
MMJJ
_RS08710
+0.7
4.6
UTP--glucose-1-phosphate uridylyltransferase GalU
compare
MMJJ
_RS03205
+0.7
3.3
metallophosphoesterase
compare
MMJJ
_RS05870
+0.7
2.1
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMJJ
_RS06975
+0.7
1.8
protein translocase SEC61 complex subunit gamma
compare
MMJJ
_RS08765
+0.7
5.1
glycosyltransferase family 9 protein
compare
MMJJ
_RS05505
+0.7
6.7
hypothetical protein
compare
MMJJ
_RS01825
+0.7
2.2
DNA repair and recombination protein RadB
compare
MMJJ
_RS08780
+0.7
2.8
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
compare
MMJJ
_RS04180
+0.7
1.5
metalloregulator ArsR/SmtB family transcription factor
compare
MMJJ
_RS05510
+0.7
3.8
archaellum operon transcriptional activator EarA family protein
compare
MMJJ
_RS06055
+0.7
3.8
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMJJ
_RS05980
+0.7
3.3
DUF5400 domain-containing protein
compare
MMJJ
_RS09050
+0.7
2.1
hypothetical protein
compare
MMJJ
_RS03725
+0.6
1.9
sulfurtransferase TusA family protein
compare
MMJJ
_RS07280
+0.6
3.8
phosphomannomutase/phosphoglucomutase
compare
MMJJ
_RS03185
+0.6
0.9
alanine--tRNA ligase
compare
MMJJ
_RS05035
+0.6
3.6
4Fe-4S binding protein
compare
MMJJ
_RS06105
+0.6
3.4
DUF99 family protein
compare
MMJJ
_RS08785
+0.6
2.7
D-sedoheptulose 7-phosphate isomerase
compare
MMJJ
_RS05700
+0.6
3.6
metallophosphoesterase
compare
MMJJ
_RS08025
+0.6
1.7
hypothetical protein
compare
MMJJ
_RS01010
+0.6
5.3
right-handed parallel beta-helix repeat-containing protein
compare
MMJJ
_RS06465
+0.6
3.3
hypothetical protein
compare
MMJJ
_RS05335
+0.6
2.5
heavy metal-binding domain-containing protein
compare
MMJJ
_RS04825
+0.5
4.2
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
compare
MMJJ
_RS05695
+0.5
3.1
class III signal peptide-containing protein
compare
MMJJ
_RS05690
+0.5
4.7
hypothetical protein
compare
MMJJ
_RS04525
+0.5
2.2
hypothetical protein
compare
MMJJ
_RS06205
+0.5
2.8
EamA family transporter
compare
MMJJ
_RS06430
+0.5
2.7
haloacid dehalogenase
compare
MMJJ
_RS01920
+0.5
3.7
phosphoglycerate mutase
compare
MMJJ
_RS07270
+0.5
2.9
ribonuclease HII
compare
MMJJ
_RS04055
+0.5
2.1
hypothetical protein
compare
MMJJ
_RS08065
+0.5
0.7
precorrin-6Y C5,15-methyltransferase (decarboxylating) subunit CbiT
compare
MMJJ
_RS06740
+0.5
2.2
energy-coupling factor ABC transporter substrate-binding protein
compare
MMJJ
_RS06135
+0.5
3.3
50S ribosomal protein L11 methyltransferase
compare
MMJJ
_RS02860
+0.5
1.0
site-specific integrase
compare
MMJJ
_RS04135
+0.5
2.4
7-cyano-7-deazaguanine synthase QueC
compare
MMJJ
_RS05925
+0.5
2.5
DUF2099 family protein
compare
MMJJ
_RS07825
+0.5
1.7
YciI family protein
compare
MMJJ
_RS00440
+0.5
2.9
selenide, water dikinase SelD
compare
MMJJ
_RS06755
+0.5
3.0
diadenylate cyclase
compare
MMJJ
_RS07640
+0.5
4.0
cache domain-containing protein
compare
MMJJ
_RS07680
+0.4
2.9
glucose-6-phosphate isomerase
compare
MMJJ
_RS06275
+0.4
2.0
hypothetical protein
compare
MMJJ
_RS06450
+0.4
2.8
DUF3343 domain-containing protein
compare
MMJJ
_RS05985
+0.4
2.7
ABC transporter substrate-binding protein
compare
MMJJ
_RS07740
+0.4
2.4
DUF2334 domain-containing protein
compare
MMJJ
_RS06115
+0.4
2.9
pyruvate kinase
compare
MMJJ
_RS05915
+0.4
1.0
multiprotein bridging factor aMBF1
compare
MMJJ
_RS06630
+0.4
3.2
4Fe-4S dicluster domain-containing protein
compare
MMJJ
_RS03350
+0.4
0.8
hypothetical protein
compare
MMJJ
_RS06585
+0.4
2.5
alanine racemase
compare
MMJJ
_RS04245
+0.4
2.2
flavoprotein
compare
MMJJ
_RS06720
+0.4
2.8
mechanosensitive ion channel family protein
compare
MMJJ
_RS06470
+0.4
2.1
tripartite tricarboxylate transporter permease
compare
MMJJ
_RS09390
+0.4
0.7
hypothetical protein
compare
MMJJ
_RS00170
+0.4
1.8
thioredoxin family protein
compare
MMJJ
_RS04875
+0.4
0.5
tRNA-Lys
compare
MMJJ
_RS09325
+0.4
1.6
hypothetical protein
compare
MMJJ
_RS09070
+0.4
1.8
hypothetical protein
compare
MMJJ
_RS03285
+0.4
1.5
gamma-glutamylcyclotransferase
compare
MMJJ
_RS05730
+0.4
2.7
TIM barrel protein
compare
MMJJ
_RS07635
+0.4
2.1
response regulator transcription factor
compare
MMJJ
_RS05525
+0.4
1.9
GTP-binding protein
compare
MMJJ
_RS06190
+0.4
2.7
7-cyano-7-deazaguanine synthase
compare
MMJJ
_RS08705
+0.4
2.4
AEC family transporter
compare
MMJJ
_RS05365
+0.4
1.9
DUF2798 domain-containing protein
compare
MMJJ
_RS06770
+0.4
2.8
hypothetical protein
compare
MMJJ
_RS04935
+0.4
1.6
4Fe-4S binding protein
compare
MMJJ
_RS05495
+0.4
2.3
MinD/ParA family protein
compare
MMJJ
_RS06495
+0.4
0.8
hypothetical protein
compare
MMJJ
_RS05890
+0.4
2.7
molybdate ABC transporter substrate-binding protein
compare
MMJJ
_RS07905
+0.4
2.1
DUF89 domain-containing protein
compare
MMJJ
_RS05410
+0.4
1.9
RecB-family nuclease
compare
MMJJ
_RS09010
+0.4
1.7
23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
compare
MMJJ
_RS09205
+0.4
1.3
hypothetical protein
compare
MMJJ
_RS08365
+0.4
1.2
DsrE family protein
compare
MMJJ
_RS01125
+0.4
1.3
hypothetical protein
compare
MMJJ
_RS06765
+0.4
2.8
hypothetical protein
compare
MMJJ
_RS06445
+0.4
3.0
hypothetical protein
compare
MMJJ
_RS07385
+0.4
2.3
ribosomal protein S18-alanine N-acetyltransferase
compare
MMJJ
_RS07210
+0.3
2.0
AmmeMemoRadiSam system protein B
compare
MMJJ
_RS07255
+0.3
2.9
hypothetical protein
compare
MMJJ
_RS06675
+0.3
2.1
TIGR01212 family radical SAM protein
compare
MMJJ
_RS06080
+0.3
2.4
serine/threonine protein kinase
compare
MMJJ
_RS06460
+0.3
2.1
cytochrome c
compare
MMJJ
_RS03380
+0.3
1.0
hypothetical protein
compare
MMJJ
_RS05685
+0.3
1.3
class III signal peptide-containing protein
compare
MMJJ
_RS08370
+0.3
1.0
hypothetical protein
compare
MMJJ
_RS01285
+0.3
1.9
CopG family ribbon-helix-helix protein
compare
MMJJ
_RS05250
+0.3
1.7
HDIG domain-containing protein
compare
MMJJ
_RS02285
+0.3
1.2
class III signal peptide-containing protein
compare
MMJJ
_RS06160
+0.3
2.0
stage II sporulation protein M
compare
MMJJ
_RS07665
+0.3
2.7
formate dehydrogenase subunit alpha
compare
MMJJ
_RS08325
+0.3
0.4
glycosyltransferase family 2 protein
compare
MMJJ
_RS00540
+0.3
1.3
hypothetical protein
compare
MMJJ
_RS07830
+0.3
2.2
DUF1786 domain-containing protein
compare
MMJJ
_RS06120
+0.3
1.8
DUF4013 domain-containing protein
compare
MMJJ
_RS08690
+0.3
1.9
phosphate ABC transporter substrate-binding protein
compare
MMJJ
_RS01590
+0.3
0.3
adenine phosphoribosyltransferase
compare
MMJJ
_RS01955
+0.3
2.5
hypothetical protein
compare
MMJJ
_RS00420
+0.3
1.3
CBS domain-containing protein
compare
MMJJ
_RS07660
+0.3
1.5
carbonic anhydrase
compare
MMJJ
_RS06810
+0.3
2.2
L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
compare
MMJJ
_RS05965
+0.3
2.4
MFS transporter
compare
MMJJ
_RS07790
+0.3
2.0
hypothetical protein
compare
MMJJ
_RS01650
+0.3
2.5
acetolactate synthase large subunit
compare
MMJJ
_RS05240
+0.3
2.3
type II secretion system F family protein
compare
MMJJ
_RS01060
+0.3
1.2
ABC transporter ATP-binding protein
compare
MMJJ
_RS03360
+0.3
0.4
hypothetical protein
compare
MMJJ
_RS02000
+0.3
0.9
AzlD domain-containing protein
compare
MMJJ
_RS08580
+0.3
1.1
YkgJ family cysteine cluster protein
compare
MMJJ
_RS02095
+0.3
1.8
DUF2124 family protein
compare
MMJJ
_RS05190
+0.3
1.4
gamma carbonic anhydrase family protein
compare
MMJJ
_RS07610
+0.3
1.3
DUF2119 domain-containing protein
compare
MMJJ
_RS05350
+0.3
1.7
DUF192 domain-containing protein
compare
MMJJ
_RS04105
+0.3
2.1
prepilin peptidase
compare
MMJJ
_RS07500
+0.3
1.7
hypothetical protein
compare
MMJJ
_RS05195
+0.3
0.8
hypothetical protein
compare
MMJJ
_RS06440
+0.3
1.5
DUF4870 domain-containing protein
compare
MMJJ
_RS04090
+0.3
2.1
hypothetical protein
compare
MMJJ
_RS07420
+0.3
1.4
protease complex subunit PrcB family protein
compare
MMJJ
_RS08075
+0.3
1.8
ATP-dependent DNA helicase
compare
MMJJ
_RS05880
+0.3
2.2
hypothetical protein
compare
MMJJ
_RS02905
+0.3
0.9
NifB/NifX family molybdenum-iron cluster-binding protein
compare
MMJJ
_RS06960
+0.3
2.2
CAP domain-containing protein
compare
MMJJ
_RS05500
+0.3
2.2
hypothetical protein
compare
MMJJ
_RS07970
+0.3
0.3
O-phospho-L-seryl-tRNA:Cys-tRNA synthase
compare
MMJJ
_RS05885
+0.3
2.0
tungstate ABC transporter substrate-binding protein WtpA
compare
MMJJ
_RS07390
+0.3
1.8
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMJJ
_RS09405
+0.3
0.8
hypothetical protein
compare
MMJJ
_RS04440
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS09225
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS08200
+0.2
1.9
surface protein, anchor region
compare
MMJJ
_RS04780
+0.2
0.8
nucleic acid-binding protein
compare
MMJJ
_RS07920
+0.2
1.7
6-carboxytetrahydropterin synthase QueD
compare
MMJJ
_RS03690
+0.2
1.1
indolepyruvate oxidoreductase subunit beta
compare
MMJJ
_RS08385
+0.2
1.4
class II SORL domain-containing protein
compare
MMJJ
_RS07650
+0.2
1.8
formate/nitrite transporter family protein
compare
MMJJ
_RS05940
+0.2
1.4
HAD family hydrolase
compare
MMJJ
_RS07620
+0.2
1.6
class I SAM-dependent rRNA methyltransferase
compare
MMJJ
_RS03370
+0.2
0.7
hypothetical protein
compare
MMJJ
_RS09210
+0.2
1.6
lysine exporter LysO family protein
compare
MMJJ
_RS05400
+0.2
0.8
CoA-binding protein
compare
MMJJ
_RS01840
+0.2
1.3
flavodoxin family protein
compare
MMJJ
_RS07235
+0.2
1.7
coenzyme F420 hydrogenase subunit alpha
compare
MMJJ
_RS04845
+0.2
1.7
ABC transporter substrate-binding protein
compare
MMJJ
_RS01110
+0.2
0.7
hypothetical protein
compare
MMJJ
_RS06455
+0.2
1.5
YeeE/YedE family protein
compare
MMJJ
_RS05205
+0.2
1.5
DUF2118 domain-containing protein
compare
MMJJ
_RS00290
+0.2
1.4
CheF family chemotaxis protein
compare
MMJJ
_RS05820
+0.2
1.3
DUF2304 domain-containing protein
compare
MMJJ
_RS07810
+0.2
1.2
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMJJ
_RS04065
+0.2
1.4
hypothetical protein
compare
MMJJ
_RS02185
+0.2
1.2
TIGR00296 family protein
compare
MMJJ
_RS06830
+0.2
0.9
4Fe-4S binding protein
compare
MMJJ
_RS04785
+0.2
1.2
OB-fold nucleic acid binding domain-containing protein
compare
MMJJ
_RS07365
+0.2
1.4
CatB-related O-acetyltransferase
compare
MMJJ
_RS05875
+0.2
1.9
hypothetical protein
compare
MMJJ
_RS09155
+0.2
1.5
RluA family pseudouridine synthase
compare
MMJJ
_RS01380
+0.2
1.2
DUF1890 domain-containing protein
compare
MMJJ
_RS09365
+0.2
0.5
hypothetical protein
compare
MMJJ
_RS07230
+0.2
1.2
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS03700
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS03410
+0.2
0.5
hypothetical protein
compare
MMJJ
_RS05605
+0.2
0.9
hypothetical protein
compare
MMJJ
_RS06045
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS09020
+0.2
0.8
hypothetical protein
compare
MMJJ
_RS09355
+0.2
0.9
hypothetical protein
compare
MMJJ
_RS01515
+0.2
0.8
DUF2178 domain-containing protein
compare
MMJJ
_RS04955
+0.2
1.2
tRNA pseudouridine(54/55) synthase Pus10
compare
MMJJ
_RS06100
+0.2
1.2
rubrerythrin
compare
MMJJ
_RS03385
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS00870
+0.2
0.9
F420-nonreducing hydrogenase
compare
MMJJ
_RS07670
+0.2
1.6
Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain
compare
MMJJ
_RS00555
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS02005
+0.2
1.2
AzlC family ABC transporter permease
compare
MMJJ
_RS00910
+0.2
1.4
right-handed parallel beta-helix repeat-containing protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in rich t2 experiments