Experiment set2IT046 for Methanococcus maripaludis JJ
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Group: rich t2Media: McCas_MOPS, pH=7
Culturing: Methanococcus_JJ_ML1, 5 mL Balch Tube, Anaerobic, at 37 (C), shaken=180 rpm
Growth: about 9.7 generations
By: Leslie Day on 10-Oct
Media components: 22 g/L Sodium Chloride, 2.5 g/L Sodium bicarbonate, 1 g/L Ammonium chloride, 0.14 g/L Potassium phosphate dibasic, 0.0095 g/L Iron (II) sulfate heptahydrate, 0.001 g/L resazurin sodium salt, 0.84 g/L Sodium acetate, 2 g/L casamino acids, 0.5 g/L Dithiothreitol, 0.001 g/L Sodium sulfide nonahydrate, MOPS_2M_pH7 (0.002 M 3-(N-morpholino)propanesulfonic acid, 0.0005 M Sodium Hydroxide), Mc_general_salts (0.335 g/L Potassium Chloride, 2.75 g/L Magnesium chloride hexahydrate, 3.45 g/L Magnesium Sulfate Heptahydrate, 0.105 g/L Calcium chloride), Mc_trace_minerals (0.021 g/L Trisodium citrate dihydrate, 0.005 g/L manganese(II) sulfate dihydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Boric Acid, 0.001 g/L Sodium Molybdate Dihydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.002 g/L Sodium selenate, 0.0001 g/L vanadium chloride, 3.3e-05 g/L Sodium tungstate dihydrate), Mc_vitamins (2e-05 g/L biotin, 2e-05 g/L Folic Acid, 0.0001 g/L Pyridoxine HCl, 5e-05 g/L Thiamine HCl, 5e-05 g/L Riboflavin, 5e-05 g/L Nicotinic Acid, 5e-05 g/L calcium pantothenate, 1e-06 g/L Cyanocobalamin, 5e-05 g/L 4-Aminobenzoic acid, 5e-05 g/L Lipoic acid)
Specific Phenotypes
None in this experiment