Experiment set2IT044 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Phosphomycin disodium salt 0.004 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_4466 +6.1 48.3 Glycerol-3-phosphate transporter conserved
Pf6N2E2_4071 +5.5 26.4 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_4073 +5.5 34.3 Phosphate transport system permease protein PstA (TC 3.A.1.7.1) compare
Pf6N2E2_5494 +5.5 23.0 Pyruvate kinase (EC 2.7.1.40) compare
Pf6N2E2_2681 +5.2 13.9 3-phosphoglycerate kinase compare
Pf6N2E2_4074 +5.2 44.5 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_4072 +5.1 34.2 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_5574 +5.0 37.4 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_4075 +4.7 23.2 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_5156 +4.3 9.6 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_5575 +4.1 16.5 Glycerol uptake facilitator protein compare
Pf6N2E2_2277 +4.0 12.2 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_4467 +3.9 3.0 hypothetical protein compare
Pf6N2E2_4365 +3.9 22.4 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_4276 +3.8 18.0 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_4067 +3.4 11.8 Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) compare
Pf6N2E2_5520 +3.2 19.4 Sensory box histidine kinase compare
Pf6N2E2_4465 +3.2 17.4 ChaC-related protein conserved
Pf6N2E2_2800 +3.1 6.7 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) compare
Pf6N2E2_5153 +3.1 11.5 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_2872 +3.0 18.9 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_4803 +3.0 4.0 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_5030 +3.0 12.9 Transcriptional regulator, AsnC family compare
Pf6N2E2_2886 +3.0 25.1 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_4468 +2.9 8.5 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_3841 +2.7 13.3 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_4454 +2.7 15.4 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_4048 +2.6 14.9 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_4362 +2.5 5.3 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_1626 +2.4 10.4 Putative ATP-binding component of a transport system compare
Pf6N2E2_3826 +2.4 16.1 Nitrogen regulation protein NR(I) compare
Pf6N2E2_2825 +2.4 8.6 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_4047 +2.4 13.4 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4826 +2.4 13.4 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3338 +2.4 5.1 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_2680 +2.4 3.6 FMN-dependent NADH-azoreductase compare
Pf6N2E2_5155 +2.3 7.2 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_3283 +2.3 14.3 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_3842 +2.3 6.4 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_5884 +2.2 10.2 Sensor histidine kinase compare
Pf6N2E2_2824 +2.2 14.4 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_1625 +2.2 3.8 Ferric reductase (1.6.99.14) compare
Pf6N2E2_5237 +2.1 5.1 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf6N2E2_3820 +2.1 1.8 hypothetical protein compare
Pf6N2E2_445 +2.0 11.7 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_4635 +2.0 13.4 ATP-dependent RNA helicase RhlE compare
Pf6N2E2_3885 +2.0 6.7 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_5158 +1.9 3.8 FIG00954153: hypothetical protein compare
Pf6N2E2_2384 +1.9 10.2 CmpX compare
Pf6N2E2_2823 +1.9 6.5 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_5157 +1.9 8.4 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2391 +1.9 11.4 FIG137360: hypothetical protein conserved
Pf6N2E2_4120 +1.8 13.9 Trk system potassium uptake protein TrkA compare
Pf6N2E2_5243 +1.8 3.1 hypothetical protein compare
Pf6N2E2_5178 +1.8 3.2 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_5150 +1.7 9.7 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_4987 +1.7 2.1 MaoC-like domain protein compare
Pf6N2E2_3791 +1.7 11.6 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_4614 +1.7 6.2 twitching motility protein PilH compare
Pf6N2E2_3825 +1.7 3.3 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_3763 +1.7 2.6 hypothetical protein compare
Pf6N2E2_5319 +1.6 10.3 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
Pf6N2E2_4469 +1.6 9.4 Quinone oxidoreductase (EC 1.6.5.5) conserved
Pf6N2E2_5318 +1.6 14.1 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf6N2E2_2634 +1.6 11.0 Potassium uptake protein TrkH compare
Pf6N2E2_3792 +1.6 8.6 Glucans biosynthesis protein G precursor compare
Pf6N2E2_3667 +1.6 2.5 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_5317 +1.6 12.4 Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-) compare
Pf6N2E2_5321 +1.5 9.9 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf6N2E2_2181 +1.5 2.2 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_2518 +1.5 3.5 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_1056 +1.5 1.8 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_156 +1.5 4.2 Carbon storage regulator compare
Pf6N2E2_3839 +1.5 4.6 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3198 +1.5 3.6 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_5250 +1.5 2.6 Restriction endonuclease compare
Pf6N2E2_4615 +1.5 2.9 type IV pili signal transduction protein PilI compare
Pf6N2E2_4470 +1.4 10.7 Flagellar biosynthesis protein FliL conserved
Pf6N2E2_5320 +1.4 7.1 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf6N2E2_2754 +1.4 6.0 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf6N2E2_5064 +1.4 7.9 Xanthine/uracil/thiamine/ascorbate permease family protein compare
Pf6N2E2_3875 +1.4 1.8 FIG00953324: hypothetical protein compare
Pf6N2E2_5705 +1.3 2.1 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_2018 +1.3 2.8 hypothetical protein compare
Pf6N2E2_4476 +1.3 6.7 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
Pf6N2E2_2967 +1.3 2.6 Cold shock protein CspA compare
Pf6N2E2_5714 +1.3 3.3 Flavoprotein WrbA compare
Pf6N2E2_5175 +1.3 10.2 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_5768 +1.2 3.6 DNA recombination-dependent growth factor C compare
Pf6N2E2_3574 +1.2 9.4 Mannose-6-phosphate isomerase (EC 5.3.1.8) conserved
Pf6N2E2_827 +1.2 2.3 probable dioxygenase compare
Pf6N2E2_4618 +1.2 4.5 Chemotaxis signal transduction protein compare
Pf6N2E2_4593 +1.2 3.7 Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB compare
Pf6N2E2_5758 +1.2 3.3 FIG00957314: hypothetical protein compare
Pf6N2E2_4359 +1.2 5.4 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2755 +1.2 2.2 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_4155 +1.2 8.3 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf6N2E2_3634 +1.2 1.4 Transporter compare
Pf6N2E2_5666 +1.2 7.2 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_4617 +1.2 9.4 Signal transduction histidine kinase CheA (EC 2.7.3.-) compare
Pf6N2E2_4631 +1.2 8.0 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_4150 +1.2 5.2 probable exported protein YPO0432 compare
Pf6N2E2_525 +1.2 6.0 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_2999 +1.2 2.9 probable tail fiber assembly protein compare
Pf6N2E2_4759 +1.2 8.7 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_1484 +1.2 3.5 N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) compare
Pf6N2E2_3539 +1.2 4.4 FIG00955840: hypothetical protein compare
Pf6N2E2_5976 +1.2 3.1 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21) compare
Pf6N2E2_1434 +1.1 1.3 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_2003 +1.1 3.4 hypothetical protein compare
Pf6N2E2_4763 +1.1 6.6 Biotin synthesis protein BioC compare
Pf6N2E2_3144 +1.1 7.5 Thiol:disulfide interchange protein DsbC compare
Pf6N2E2_3258 +1.1 4.2 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_4471 +1.1 6.9 ABC-type multidrug transport system, permease component compare
Pf6N2E2_82 +1.1 1.8 hypothetical protein compare
Pf6N2E2_2285 +1.1 1.9 FIG00954079: hypothetical protein compare
Pf6N2E2_5018 +1.1 7.4 tRNA-i(6)A37 methylthiotransferase compare
Pf6N2E2_5176 +1.1 6.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_2219 +1.1 1.5 Cell division trigger factor (EC 5.2.1.8) compare
Pf6N2E2_4601 +1.1 1.5 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_942 +1.1 1.7 Mll6465 protein compare
Pf6N2E2_5749 +1.1 4.3 hypothetical protein compare
Pf6N2E2_1656 +1.1 2.1 hypothetical protein compare
Pf6N2E2_5207 +1.1 1.5 hypothetical protein compare
Pf6N2E2_5771 +1.1 5.2 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_4024 +1.1 6.3 L-Proline/Glycine betaine transporter ProP compare
Pf6N2E2_2474 +1.0 6.9 ErfK/YbiS/YcfS/YnhG family protein compare
Pf6N2E2_5406 +1.0 2.4 FIG00958224: hypothetical protein compare
Pf6N2E2_3285 +1.0 3.9 Rod shape-determining protein MreD compare
Pf6N2E2_3672 +1.0 4.5 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) compare
Pf6N2E2_2067 +1.0 8.8 cis/trans isomerase compare
Pf6N2E2_4762 +1.0 3.2 Biotin synthesis protein BioH compare
Pf6N2E2_54 +1.0 3.0 Transcriptional regulator, TetR family compare
Pf6N2E2_3237 +1.0 2.7 hypothetical protein compare
Pf6N2E2_750 +1.0 2.9 Transcriptional regulator, DeoR family compare
Pf6N2E2_5723 +1.0 7.1 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_4761 +1.0 5.9 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_5583 +1.0 3.9 Bacterioferritin compare
Pf6N2E2_2425 +1.0 2.5 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2447 +1.0 3.7 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_5044 +1.0 3.6 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_3179 +1.0 1.4 FIG021952: putative membrane protein compare
Pf6N2E2_5722 +1.0 4.0 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939 compare
Pf6N2E2_5337 +1.0 3.3 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_902 +1.0 1.9 hypothetical protein compare
Pf6N2E2_4459 +1.0 2.1 Transcriptional regulator, GntR family compare
Pf6N2E2_4764 +1.0 4.1 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_1034 +1.0 1.1 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_1095 +1.0 1.6 Opine oxidase subunit C compare
Pf6N2E2_4153 +1.0 0.7 Sensory box histidine kinase compare
Pf6N2E2_3940 +1.0 4.9 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5160 +0.9 1.0 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_3521 +0.9 6.4 Sensor histidine kinase/response regulator compare
Pf6N2E2_5785 +0.9 2.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2699 +0.9 4.1 Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO compare
Pf6N2E2_1249 +0.9 2.9 Transcriptional regulator, AraC family compare
Pf6N2E2_5865 +0.9 6.5 Chaperone protein HtpG compare
Pf6N2E2_2278 +0.9 6.7 Transcription-repair coupling factor compare
Pf6N2E2_2845 +0.9 5.1 RNA polymerase associated protein RapA (EC 3.6.1.-) compare
Pf6N2E2_4821 +0.9 3.9 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf6N2E2_4305 +0.9 1.2 FIG00959590: hypothetical protein compare
Pf6N2E2_4556 +0.9 2.0 FIG00953078: hypothetical protein compare
Pf6N2E2_4889 +0.9 3.7 Putative transport protein compare
Pf6N2E2_4658 +0.9 1.2 FIG00955006: hypothetical protein compare
Pf6N2E2_5425 +0.8 0.6 hypothetical protein compare
Pf6N2E2_4083 +0.8 2.0 hypothetical protein compare
Pf6N2E2_2727 +0.8 4.2 Ribonuclease D (EC 3.1.26.3) compare
Pf6N2E2_2869 +0.8 1.6 Peptide deformylase (EC 3.5.1.88) compare
Pf6N2E2_37 +0.8 2.0 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_4607 +0.8 2.9 Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR compare
Pf6N2E2_2524 +0.8 4.1 S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase compare
Pf6N2E2_924 +0.8 1.8 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_757 +0.8 4.8 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) compare
Pf6N2E2_891 +0.8 2.5 4-oxalocrotonate tautomerase compare
Pf6N2E2_3475 +0.8 1.1 hypothetical protein compare
Pf6N2E2_3762 +0.8 4.5 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_5188 +0.8 3.0 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_3652 +0.8 5.0 Cation transport ATPase compare
Pf6N2E2_3807 +0.8 4.0 Histidine utilization repressor compare
Pf6N2E2_2459 +0.8 1.9 Organic hydroperoxide resistance protein compare
Pf6N2E2_2885 +0.8 2.6 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_1984 +0.8 3.1 probable exported protein STY4558 compare
Pf6N2E2_5312 +0.8 1.5 FIG00964214: hypothetical protein compare
Pf6N2E2_4654 +0.8 2.7 hypothetical protein compare
Pf6N2E2_1371 +0.8 2.5 hypothetical protein compare
Pf6N2E2_4661 +0.8 3.1 KluA regulatory protein compare
Pf6N2E2_5728 +0.8 2.5 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_4059 +0.8 1.2 DNA-binding protein HU-alpha compare
Pf6N2E2_2465 +0.8 4.3 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2471 +0.8 4.6 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_4025 +0.8 1.1 hypothetical protein compare
Pf6N2E2_3737 +0.8 1.7 General secretion pathway protein M compare
Pf6N2E2_4638 +0.8 2.3 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_5339 +0.8 3.4 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_5177 +0.8 5.1 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3845 +0.8 4.9 A/G-specific adenine glycosylase (EC 3.2.2.-) compare
Pf6N2E2_1040 +0.7 1.5 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_3104 +0.7 1.8 FIG00958830: hypothetical protein compare
Pf6N2E2_4804 +0.7 2.4 ApaG protein compare
Pf6N2E2_146 +0.7 1.3 Transcriptional regulator, MarR family compare


Specific Phenotypes

For 32 genes in this experiment

For stress Phosphomycin disodium salt in Pseudomonas fluorescens FW300-N2E2

For stress Phosphomycin disodium salt across organisms