Experiment set2IT044 for Pseudomonas fluorescens FW300-N2E2

LB with Phosphomycin disodium salt 0.004 mg/ml

Group: stress
Media: LB + Phosphomycin disodium salt (0.004 mg/ml)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1183 D1,D2

Specific Phenotypes

For 32 genes in this experiment

For stress Phosphomycin disodium salt in Pseudomonas fluorescens FW300-N2E2

For stress Phosphomycin disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	6
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Arginine Biosynthesis extended	1
Arginine and Ornithine Degradation	1
De Novo Pyrimidine Synthesis	1
Flagellum	1
Fosfomycin resistance	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycine Biosynthesis	1
Glycine and Serine Utilization	1
Hfl operon	1
LMPTP YwlE cluster	1
Lysine degradation	1
Mannose Metabolism	1
Peptidoglycan Biosynthesis	1
Purine Utilization	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1
Ribosomal protein S12p Asp methylthiotransferase	1
Type IV pilus	1
Universal GTPases	1
YjeE	1
ZZ gjo need homes	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Peptidoglycan biosynthesis
• Puromycin biosynthesis
• Pyrimidine metabolism
• Alanine and aspartate metabolism
• Glycine, serine and threonine metabolism
• Geraniol degradation
• Lysine biosynthesis
• Lysine degradation
• Tryptophan metabolism
• Cyanoamino acid metabolism
• Polyketide sugar unit biosynthesis
• Lipopolysaccharide biosynthesis
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Biotin metabolism
• Limonene and pinene degradation
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
alginate biosynthesis I (algal)	3	3	3
benzene degradation IV (anaerobic)	1	1	1
pyrimidine nucleobases salvage I	1	1	1
L-asparagine degradation I	1	1	1
alginate biosynthesis II (bacterial)	7	6	4
pyrimidine nucleobases salvage II	2	2	1
D-mannose degradation II	2	1	1
L-threonine degradation II	2	1	1
D-mannose degradation I	2	1	1
L-asparagine degradation III (mammalian)	3	2	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	1	1
GDP-mannose biosynthesis	4	4	1
superpathway of pyrimidine nucleobases salvage	4	4	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	3	1
mannitol degradation II	4	2	1
L-ornithine biosynthesis I	5	5	1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation	5	2	1
1,5-anhydrofructose degradation	5	2	1
β-(1,4)-mannan degradation	7	2	1
alginate degradation	7	2	1
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
peptidoglycan recycling II	10	8	1
superpathway of pyrimidine ribonucleosides salvage	10	6	1
colanic acid building blocks biosynthesis	11	9	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
superpathway of arginine and polyamine biosynthesis	17	14	1
superpathway of L-threonine metabolism	18	13	1