Experiment set2IT043 for Pseudomonas fluorescens FW300-N1B4

Compare to:

LB with Cisplatin 0.006 mg/ml

200 most important genes:

  gene name fitness t score description  
Pf1N1B4_5618 -4.5 -3.1 Excinuclease ABC subunit C compare
Pf1N1B4_2005 -3.6 -2.5 ATP-dependent DNA helicase UvrD/PcrA compare
Pf1N1B4_890 -3.3 -2.2 DNA recombination and repair protein RecO compare
Pf1N1B4_1180 -3.0 -2.8 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf1N1B4_3864 -2.9 -5.4 Recombination protein RecR compare
Pf1N1B4_26 -2.7 -7.9 Excinuclease ABC subunit B compare
Pf1N1B4_5224 -2.6 -3.7 tRNA-Arg-CCT compare
Pf1N1B4_1927 -2.6 -9.1 DNA recombination and repair protein RecF conserved
Pf1N1B4_2611 -2.5 -13.1 Excinuclease ABC subunit A conserved
Pf1N1B4_4051 -2.3 -5.3 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf1N1B4_4640 -2.3 -5.9 MoxR-like ATPase in aerotolerance operon compare
Pf1N1B4_2105 -2.3 -3.3 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf1N1B4_4636 -2.3 -3.1 TPR domain protein in aerotolerance operon compare
Pf1N1B4_2004 -2.3 -2.5 putative membrane protein compare
Pf1N1B4_4934 -2.2 -7.4 DNA damage response nuclease (from data) conserved
Pf1N1B4_2412 -2.2 -3.9 ATP-dependent 23S rRNA helicase DbpA compare
Pf1N1B4_2621 -2.1 -2.6 PspA/IM30 family protein compare
Pf1N1B4_1234 -2.0 -4.5 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) compare
Pf1N1B4_2959 -1.9 -5.1 FIG067310: hypothetical protein compare
Pf1N1B4_3222 -1.9 -2.4 Ferredoxin compare
Pf1N1B4_1608 -1.9 -3.9 FIG00956533: hypothetical protein compare
Pf1N1B4_2682 -1.9 -2.5 Antiholin-like protein LrgA compare
Pf1N1B4_1565 -1.8 -3.9 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf1N1B4_5146 -1.8 -3.0 Chromosome initiation inhibitor compare
Pf1N1B4_3011 -1.8 -2.5 Tyrosine recombinase XerD compare
Pf1N1B4_4935 -1.8 -6.9 DinG family ATP-dependent helicase CPE1197 conserved
Pf1N1B4_1619 -1.7 -9.2 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf1N1B4_769 -1.7 -7.4 Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) compare
Pf1N1B4_2106 -1.7 -3.6 Hydrogen peroxide-inducible genes activator compare
Pf1N1B4_5450 -1.7 -8.9 Error-prone, lesion bypass DNA polymerase V (UmuC) conserved
Pf1N1B4_2612 -1.7 -3.8 Putative transport protein compare
Pf1N1B4_3916 -1.7 -10.0 Transcription-repair coupling factor conserved
Pf1N1B4_1403 -1.7 -7.1 3'-to-5' exoribonuclease RNase R compare
Pf1N1B4_3483 -1.6 -5.4 Transcriptional regulatory protein PhoP compare
Pf1N1B4_3812 -1.6 -7.6 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10) compare
Pf1N1B4_3091 -1.6 -4.6 Membrane-associated zinc metalloprotease compare
Pf1N1B4_5797 -1.6 -2.8 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25) compare
Pf1N1B4_2917 -1.6 -7.5 FIG140336: TPR domain protein compare
Pf1N1B4_3447 -1.6 -5.0 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf1N1B4_1626 -1.6 -7.1 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf1N1B4_888 -1.6 -4.3 Transglycosylase, Slt family compare
Pf1N1B4_2352 -1.5 -5.8 Glutathione synthetase (EC 6.3.2.3) compare
Pf1N1B4_3991 -1.5 -2.2 Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family compare
Pf1N1B4_5619 -1.5 -3.7 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_1845 -1.5 -3.7 FIG00954395: hypothetical protein compare
Pf1N1B4_1307 -1.5 -2.4 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
Pf1N1B4_4544 -1.5 -1.7 Uncharacterized protein ImpA compare
Pf1N1B4_2037 -1.5 -4.0 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf1N1B4_6038 -1.5 -3.9 FIG00953874: hypothetical protein compare
Pf1N1B4_4611 -1.5 -3.2 GlpG protein (membrane protein of glp regulon) compare
Pf1N1B4_959 -1.4 -10.1 Stringent starvation protein A compare
Pf1N1B4_419 -1.4 -2.2 Transcriptional regulator, TetR family compare
Pf1N1B4_1661 -1.4 -4.7 Agmatine deiminase (EC 3.5.3.12) compare
Pf1N1B4_2768 -1.4 -3.9 DNA repair protein RadA compare
Pf1N1B4_2026 -1.4 -3.2 Transcriptional regulator compare
Pf1N1B4_3833 -1.4 -6.5 Major porin and structural outer membrane porin OprF compare
Pf1N1B4_3775 -1.4 -2.4 FIG00956876: hypothetical protein compare
Pf1N1B4_958 -1.4 -2.4 Stringent starvation protein B compare
Pf1N1B4_3143 -1.4 -2.5 RecA protein compare
Pf1N1B4_4979 -1.4 -2.6 Transcriptional regulator, AsnC family compare
Pf1N1B4_3508 -1.4 -3.4 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_6039 -1.3 -5.3 Endonuclease I precursor (EC 3.1.21.1) conserved
Pf1N1B4_4474 -1.3 -2.8 transcription regulator protein compare
Pf1N1B4_479 -1.3 -3.2 Butyryl-CoA dehydrogenase (EC 1.3.99.2) compare
Pf1N1B4_1624 -1.3 -7.1 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf1N1B4_3590 -1.3 -5.7 hypothetical protein compare
Pf1N1B4_3591 -1.3 -6.7 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41) compare
Pf1N1B4_4759 -1.3 -2.6 Proteinase inhibitor I11, ecotin precursor compare
Pf1N1B4_2283 -1.3 -2.1 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf1N1B4_2888 -1.3 -4.1 FIG00961017: hypothetical protein compare
Pf1N1B4_1402 -1.3 -3.0 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-) compare
Pf1N1B4_5013 -1.3 -2.2 Cointegrate resolution protein T compare
Pf1N1B4_2526 -1.3 -9.6 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf1N1B4_2493 -1.2 -3.2 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf1N1B4_3491 -1.2 -3.1 tRNA(Cytosine32)-2-thiocytidine synthetase compare
Pf1N1B4_1178 -1.2 -4.5 DNA repair protein RecN compare
Pf1N1B4_1236 -1.2 -5.4 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf1N1B4_846 -1.2 -1.6 hypothetical protein compare
Pf1N1B4_644 -1.2 -2.1 Cold shock protein CspC compare
Pf1N1B4_4659 -1.2 -1.7 Putative outer membrane lipoprotein compare
Pf1N1B4_2339 -1.2 -1.6 COG1872 compare
Pf1N1B4_3519 -1.2 -3.8 Lipase (EC 3.1.1.3) compare
Pf1N1B4_578 -1.2 -2.5 enoyl-CoA hydratase, R-specific compare
Pf1N1B4_683 -1.2 -3.3 Fumarate hydratase class I, aerobic (EC 4.2.1.2) compare
Pf1N1B4_2853 -1.1 -2.2 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_2244 -1.1 -1.8 Taurine-binding periplasmic protein TauA compare
Pf1N1B4_4563 -1.1 -1.5 Sulfate transporter compare
Pf1N1B4_1252 -1.1 -3.4 Adenosine deaminase (EC 3.5.4.4) compare
Pf1N1B4_224 -1.1 -1.8 Isohexenylglutaconyl-CoA hydratase compare
Pf1N1B4_345 -1.1 -1.7 Integration host factor beta subunit compare
Pf1N1B4_962 -1.1 -6.7 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf1N1B4_131 -1.1 -1.7 hypothetical protein compare
Pf1N1B4_3467 -1.1 -2.3 Flagellar biosynthesis protein FlgN compare
Pf1N1B4_547 -1.1 -3.1 3-phosphoglycerate kinase compare
Pf1N1B4_4446 -1.1 -1.5 DNA topoisomerase III (EC 5.99.1.2) compare
Pf1N1B4_2757 -1.1 -3.0 DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31 compare
Pf1N1B4_69 -1.1 -2.4 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf1N1B4_1812 -1.1 -3.1 Iron siderophore sensor protein compare
Pf1N1B4_76 -1.1 -1.8 ATP binding protein compare
Pf1N1B4_3072 -1.1 -2.8 hypothetical protein compare
Pf1N1B4_532 -1.1 -2.7 Transcriptional regulators, LysR family compare
Pf1N1B4_4669 -1.1 -1.8 Heavy metal RND efflux outer membrane protein, CzcC family compare
Pf1N1B4_3917 -1.1 -3.0 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf1N1B4_1781 -1.0 -2.1 hypothetical protein compare
Pf1N1B4_974 -1.0 -2.6 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf1N1B4_3700 -1.0 -3.0 Putative permease often clustered with de novo purine synthesis compare
Pf1N1B4_2552 -1.0 -2.9 OsmC/Ohr family protein compare
Pf1N1B4_2715 -1.0 -5.8 hypothetical protein compare
Pf1N1B4_1730 -1.0 -2.1 Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1) compare
Pf1N1B4_1116 -1.0 -6.7 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf1N1B4_4393 -1.0 -1.5 Ferredoxin reductase compare
Pf1N1B4_525 -1.0 -3.0 Agmatinase (EC 3.5.3.11) compare
Pf1N1B4_4028 -1.0 -2.8 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) compare
Pf1N1B4_1627 -1.0 -3.4 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf1N1B4_3687 -1.0 -2.2 FIG00953885: hypothetical protein compare
Pf1N1B4_3166 -1.0 -6.3 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_5976 -1.0 -1.9 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22) compare
Pf1N1B4_2322 -1.0 -2.0 FIG015547: peptidase, M16 family compare
Pf1N1B4_5682 -1.0 -2.1 SOS-response repressor and protease LexA (EC 3.4.21.88) compare
Pf1N1B4_2791 -1.0 -2.0 Putative inner membrane protein (Fragment) compare
Pf1N1B4_1613 -1.0 -2.3 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf1N1B4_1126 -1.0 -2.4 Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2) compare
Pf1N1B4_3752 -1.0 -6.8 Aspartyl aminopeptidase conserved
Pf1N1B4_2874 -1.0 -1.8 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf1N1B4_5898 -1.0 -2.3 hypothetical protein compare
Pf1N1B4_1617 -1.0 -4.5 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) compare
Pf1N1B4_5073 -1.0 -2.5 Hydroxypyruvate isomerase (EC 5.3.1.22) compare
Pf1N1B4_5659 -1.0 -6.3 NfuA Fe-S protein maturation compare
Pf1N1B4_4040 -0.9 -2.5 NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3) compare
Pf1N1B4_602 -0.9 -2.6 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf1N1B4_5675 -0.9 -1.5 hypothetical protein compare
Pf1N1B4_2935 -0.9 -2.0 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf1N1B4_3241 -0.9 -1.5 hypothetical protein compare
Pf1N1B4_3551 -0.9 -3.3 COG0613, Predicted metal-dependent phosphoesterases (PHP family) compare
Pf1N1B4_4193 -0.9 -3.2 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf1N1B4_1340 -0.9 -1.9 ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component compare
Pf1N1B4_1971 -0.9 -2.3 SAM-dependent methyltransferases compare
Pf1N1B4_3552 -0.9 -2.1 Hypothetical YciO protein, TsaC/YrdC paralog compare
Pf1N1B4_1511 -0.9 -1.3 FIG027190: Putative transmembrane protein compare
Pf1N1B4_3656 -0.9 -1.5 Eukaryotic-type low-affinity urea transporter compare
Pf1N1B4_1754 -0.9 -3.2 Putative stomatin/prohibitin-family membrane protease subunit aq_911 compare
Pf1N1B4_2491 -0.9 -2.6 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf1N1B4_3563 -0.9 -1.6 hypothetical protein compare
Pf1N1B4_5747 -0.9 -2.1 Molybdopterin biosynthesis protein MoeA compare
Pf1N1B4_4381 -0.9 -2.1 Transcriptional regulator, LysR family compare
Pf1N1B4_4176 -0.9 -1.7 Alcohol dehydrogenase (EC 1.1.1.1) compare
Pf1N1B4_1115 -0.9 -5.7 Dna binding response regulator PrrA (RegA) compare
Pf1N1B4_4195 -0.9 -2.6 Transcriptional regulator, TetR family compare
Pf1N1B4_4740 -0.9 -1.9 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf1N1B4_3145 -0.9 -2.4 Decarboxylase family protein compare
Pf1N1B4_2712 -0.9 -3.5 tRNA-i(6)A37 methylthiotransferase compare
Pf1N1B4_1936 -0.9 -3.2 Chromosome (plasmid) partitioning protein ParA compare
Pf1N1B4_3212 -0.9 -1.8 hypothetical protein compare
Pf1N1B4_1708 -0.9 -2.6 Efflux transporter, RND family, MFP subunit compare
Pf1N1B4_2502 -0.9 -3.6 Coenzyme PQQ synthesis protein B compare
Pf1N1B4_2456 -0.9 -2.1 Sarcosine oxidase delta subunit (EC 1.5.3.1) compare
Pf1N1B4_2377 -0.9 -4.1 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf1N1B4_652 -0.9 -5.4 Sensory box histidine kinase compare
Pf1N1B4_5365 -0.8 -1.8 hypothetical protein compare
Pf1N1B4_4612 -0.8 -1.7 FIG00954117: hypothetical protein compare
Pf1N1B4_448 -0.8 -1.2 Aminodeoxychorismate lyase (EC 4.1.3.38) compare
Pf1N1B4_961 -0.8 -6.6 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf1N1B4_6037 -0.8 -1.8 L-asparaginase (EC 3.5.1.1) compare
Pf1N1B4_2085 -0.8 -2.0 Uncharacterized protein similar to VCA0109 compare
Pf1N1B4_535 -0.8 -2.6 FIG00953341: hypothetical protein compare
Pf1N1B4_5072 -0.8 -2.1 Glycerol-3-phosphate regulon repressor compare
Pf1N1B4_5575 -0.8 -1.9 hypothetical protein compare
Pf1N1B4_724 -0.8 -1.6 DNA-binding response regulator ColR compare
Pf1N1B4_5602 -0.8 -2.8 6-aminohexanoate-dimer hydrolase (EC 3.5.1.46) compare
Pf1N1B4_1446 -0.8 -2.5 Predicted hydroxymethylpyrimidine transporter CytX compare
Pf1N1B4_3093 -0.8 -1.3 Outer membrane protein H precursor compare
Pf1N1B4_4425 -0.8 -2.8 Aspartate aminotransferase (EC 2.6.1.1) compare
Pf1N1B4_4242 -0.8 -2.8 cell processes; transport of small molecules; amino acids, amines, peptides compare
Pf1N1B4_1724 -0.8 -2.1 Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25) compare
Pf1N1B4_2487 -0.8 -5.0 Biotin synthase (EC 2.8.1.6) compare
Pf1N1B4_5663 -0.8 -1.8 Periplasmic sugar-binding domain protein compare
Pf1N1B4_6035 -0.8 -1.7 D-ribose ABC transporter, substrate-binding component RbsB (from data) compare
Pf1N1B4_3767 -0.8 -2.4 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17) compare
Pf1N1B4_751 -0.8 -1.1 hypothetical protein compare
Pf1N1B4_538 -0.8 -1.5 hypothetical protein compare
Pf1N1B4_2943 -0.8 -2.1 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_5847 -0.8 -2.7 transcriptional activator FtrA compare
Pf1N1B4_1915 -0.8 -5.4 Trk system potassium uptake protein TrkA compare
Pf1N1B4_3855 -0.8 -2.2 Chromosome partition protein smc compare
Pf1N1B4_3713 -0.8 -1.6 Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21) compare
Pf1N1B4_1235 -0.8 -3.4 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf1N1B4_5309 -0.8 -1.8 hypothetical protein compare
Pf1N1B4_2384 -0.8 -5.1 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf1N1B4_4571 -0.8 -2.5 ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) compare
Pf1N1B4_159 -0.8 -1.8 Putative threonine efflux protein compare
Pf1N1B4_2878 -0.8 -4.5 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_4594 -0.8 -1.7 Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) compare
Pf1N1B4_3547 -0.8 -1.6 DNA-binding response regulator compare
Pf1N1B4_4632 -0.8 -2.1 Exonuclease SbcC compare
Pf1N1B4_1042 -0.8 -1.1 Dihydroneopterin triphosphate epimerase compare
Pf1N1B4_1411 -0.8 -2.6 GTP-binding protein HflX compare
Pf1N1B4_4299 -0.8 -1.9 sensor histidine kinase compare
Pf1N1B4_5278 -0.8 -1.0 hypothetical protein compare
Pf1N1B4_5724 -0.8 -1.6 High-affinity iron permease compare
Pf1N1B4_1090 -0.8 -3.6 TldE protein, part of TldE/TldD proteolytic complex compare


Specific Phenotypes

For 8 genes in this experiment

For stress Cisplatin in Pseudomonas fluorescens FW300-N1B4

For stress Cisplatin across organisms