Experiment set2IT042 for Pseudomonas fluorescens FW300-N2E2

LB with Aluminum chloride hydrate 4 mM

Group: stress
Media: LB + Aluminum chloride hydrate (4 mM)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1183 B7,B8

Specific Phenotypes

For 10 genes in this experiment

For stress Aluminum chloride hydrate in Pseudomonas fluorescens FW300-N2E2

For stress Aluminum chloride hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Cell Division	1
Bacterial Cytoskeleton	1
Chorismate Synthesis	1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)	1
Lipid A modifications	1
Periplasmic disulfide interchange	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Phenylalanine, tyrosine and tryptophan biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-dehydroquinate biosynthesis I	2	2	1
chorismate biosynthesis I	7	7	1
superpathway of L-phenylalanine biosynthesis	10	10	1
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-tryptophan biosynthesis	13	13	1
superpathway of aromatic amino acid biosynthesis	18	18	1
superpathway of chorismate metabolism	59	43	1