Experiment set2IT042 for Pseudomonas fluorescens FW300-N1B4

LB with Thallium(I) acetate 0.008 mg/ml

Group: stress
Media: LB + Thallium(I) acetate (0.008 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Jayashree on 4/23/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 890 D7,D8

Specific Phenotypes

For 6 genes in this experiment

For stress Thallium(I) acetate in Pseudomonas fluorescens FW300-N1B4

For stress Thallium(I) acetate across organisms

SEED Subsystems

Subsystem	#Specific
Choline and Betaine Uptake and Betaine Biosynthesis	3
Aromatic amino acid degradation	1
Biogenesis of cytochrome c oxidases	1
Pterin biosynthesis	1
Serine-glyoxylate cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Photosynthesis
• Glycine, serine and threonine metabolism
• Phenylalanine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-tyrosine biosynthesis IV	1	1	1
L-phenylalanine degradation I (aerobic)	1	1	1
choline degradation I	2	2	1
glycine betaine biosynthesis II (Gram-positive bacteria)	2	2	1
glycine betaine biosynthesis I (Gram-negative bacteria)	2	2	1
choline-O-sulfate degradation	3	3	1
L-phenylalanine degradation V	3	2	1
photosynthesis light reactions	4	1	1
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane)	4	1	1
superpathway of photosynthetic hydrogen production	6	2	1
oxygenic photosynthesis	17	11	1
ethene biosynthesis V (engineered)	25	19	1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	19	1
1-butanol autotrophic biosynthesis (engineered)	27	19	1