Experiment set2IT041 for Shewanella loihica PV-4

LB with Sodium nitrite 200 mM

Group: stress
Media: LB + Sodium nitrite (200 mM)
Culturing: PV4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 12/11/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 811 D3,D4

Specific Phenotypes

For 2 genes in this experiment

For stress Sodium nitrite in Shewanella loihica PV-4

For stress Sodium nitrite across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-adenosyl-L-methionine salvage II	3	2	1
S-adenosyl-L-methionine salvage I	4	4	1
L-methionine biosynthesis III	4	3	1
L-methionine biosynthesis I	5	5	1
seleno-amino acid biosynthesis (plants)	5	3	1
L-methionine biosynthesis II	6	5	1
superpathway of L-homoserine and L-methionine biosynthesis	8	8	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	9	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	9	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	11	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
superpathway of seleno-compound metabolism	19	8	1
aspartate superpathway	25	24	1