Experiment set2IT039 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Benzethonium chloride 0.02 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_3659 +2.6 10.8 Protein fixF compare
Pf6N2E2_3663 +2.5 18.2 Glycosyl transferase compare
Pf6N2E2_3661 +2.4 11.3 hypothetical protein compare
Pf6N2E2_3664 +2.1 12.2 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_3264 +1.7 4.2 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3658 +1.7 9.6 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_3829 +1.5 9.3 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf6N2E2_4803 +1.4 2.1 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_773 +1.2 6.9 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_4677 +1.2 9.6 Choline dehydrogenase (EC 1.1.99.1) compare
Pf6N2E2_4974 +1.2 7.8 DedA protein compare
Pf6N2E2_5739 +1.1 1.4 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family compare
Pf6N2E2_942 +1.0 1.1 Mll6465 protein compare
Pf6N2E2_331 +1.0 3.7 Putative translation initiation inhibitor, yjgF family compare
Pf6N2E2_5990 +1.0 2.7 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_482 +1.0 3.0 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf6N2E2_3373 +1.0 2.3 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_4987 +1.0 1.4 MaoC-like domain protein compare
Pf6N2E2_2822 +1.0 5.9 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_6090 +0.9 2.8 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf6N2E2_6085 +0.9 2.3 Aha1 domain superfamily compare
Pf6N2E2_546 +0.9 2.5 FIG00966904: hypothetical protein compare
Pf6N2E2_5525 +0.9 7.4 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases compare
Pf6N2E2_1095 +0.9 1.5 Opine oxidase subunit C compare
Pf6N2E2_6070 +0.9 3.0 FIG137877: Hypothetical protein in pyoverdin gene cluster compare
Pf6N2E2_5647 +0.9 4.1 Transcriptional regulator, TetR family compare
Pf6N2E2_1040 +0.9 2.1 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_2440 +0.9 1.6 Free methionine-(R)-sulfoxide reductase, contains GAF domain compare
Pf6N2E2_3830 +0.9 3.4 Glutaredoxin 3 (Grx3) compare
Pf6N2E2_687 +0.8 2.9 4-oxalocrotonate tautomerase (EC 5.3.2.-) compare
Pf6N2E2_5261 +0.8 1.5 FIG00956983: hypothetical protein compare
Pf6N2E2_2509 +0.8 2.1 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_2506 +0.8 6.7 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_902 +0.8 1.1 hypothetical protein compare
Pf6N2E2_92 +0.8 2.4 hypothetical protein compare
Pf6N2E2_525 +0.8 4.3 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_1147 +0.8 2.2 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_3475 +0.8 0.9 hypothetical protein compare
Pf6N2E2_3261 +0.7 1.9 hypothetical protein compare
Pf6N2E2_5989 +0.7 6.2 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_2869 +0.7 1.2 Peptide deformylase (EC 3.5.1.88) compare
Pf6N2E2_5392 +0.7 3.2 Molybdenum cofactor biosynthesis protein MoaC compare
Pf6N2E2_3647 +0.7 3.5 LysR family transcriptional regulator PA4989 compare
Pf6N2E2_4579 +0.7 1.4 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_5571 +0.7 2.0 hypothetical protein compare
Pf6N2E2_144 +0.7 5.7 Uncharacterized protein YtfM precursor compare
Pf6N2E2_5156 +0.7 2.2 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_1323 +0.7 2.0 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_5312 +0.7 1.7 FIG00964214: hypothetical protein compare
Pf6N2E2_3328 +0.7 2.1 transcriptional regulator compare
Pf6N2E2_6060 +0.7 2.0 Propionate catabolism operon transcriptional regulator of GntR family [predicted] compare
Pf6N2E2_4121 +0.7 3.9 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf6N2E2_4679 +0.7 3.7 Betaine aldehyde dehydrogenase (EC 1.2.1.8) compare
Pf6N2E2_4359 +0.7 3.2 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_1458 +0.7 2.9 probable transporter, LysE family compare
Pf6N2E2_2999 +0.7 1.4 probable tail fiber assembly protein compare
Pf6N2E2_750 +0.6 1.8 Transcriptional regulator, DeoR family compare
Pf6N2E2_1298 +0.6 2.6 Xanthine transporter,putative compare
Pf6N2E2_2578 +0.6 1.8 Flagellar biosynthesis protein FliR compare
Pf6N2E2_2018 +0.6 2.2 hypothetical protein compare
Pf6N2E2_3569 +0.6 2.0 hypothetical protein compare
Pf6N2E2_2420 +0.6 1.7 hypothetical protein compare
Pf6N2E2_3737 +0.6 1.3 General secretion pathway protein M compare
Pf6N2E2_689 +0.6 1.3 FIG00956189: hypothetical protein compare
Pf6N2E2_3696 +0.6 1.0 hypothetical protein compare
Pf6N2E2_74 +0.6 2.0 DedD protein compare
Pf6N2E2_1817 +0.6 1.4 hypothetical protein compare
Pf6N2E2_3982 +0.6 2.3 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf6N2E2_1337 +0.6 2.0 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2503 +0.6 1.0 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_5988 +0.6 2.2 hypothetical protein compare
Pf6N2E2_2223 +0.6 1.7 hypothetical protein compare
Pf6N2E2_5816 +0.6 1.0 Smr domain protein compare
Pf6N2E2_4206 +0.6 0.9 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_522 +0.6 2.7 Inositol transport system sugar-binding protein compare
Pf6N2E2_926 +0.6 3.6 NADH dehydrogenase (EC 1.6.99.3) compare
Pf6N2E2_1917 +0.6 1.3 hypothetical protein compare
Pf6N2E2_4635 +0.6 4.0 ATP-dependent RNA helicase RhlE compare
Pf6N2E2_1656 +0.6 1.1 hypothetical protein compare
Pf6N2E2_533 +0.6 1.9 Na(+) H(+) antiporter subunit G compare
Pf6N2E2_6047 +0.6 1.2 hypothetical protein compare
Pf6N2E2_2579 +0.6 2.8 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_1676 +0.6 1.2 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_924 +0.6 1.8 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_552 +0.6 1.9 Uncharacterized protein ImpF compare
Pf6N2E2_1424 +0.6 1.4 transcriptional regulator, TetR family compare
Pf6N2E2_4442 +0.6 4.6 FIG00957574: hypothetical protein compare
Pf6N2E2_2088 +0.6 0.9 SOS-response repressor and protease LexA (EC 3.4.21.88) compare
Pf6N2E2_3179 +0.6 1.2 FIG021952: putative membrane protein compare
Pf6N2E2_5207 +0.5 1.4 hypothetical protein compare
Pf6N2E2_5986 +0.5 1.0 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5611 +0.5 3.0 SAM-dependent methyltransferases compare
Pf6N2E2_2302 +0.5 2.4 CAAX amino terminal protease family protein compare
Pf6N2E2_1774 +0.5 0.8 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_3096 +0.5 0.9 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_6022 +0.5 1.7 StbE replicon stabilization toxin compare
Pf6N2E2_2597 +0.5 3.7 Flagellar regulatory protein FleQ compare
Pf6N2E2_3720 +0.5 0.9 FIG027190: Putative transmembrane protein compare
Pf6N2E2_3122 +0.5 1.6 Chemotaxis signal transduction protein compare
Pf6N2E2_5243 +0.5 1.4 hypothetical protein compare
Pf6N2E2_2284 +0.5 1.9 Cell division inhibitor-related protein compare
Pf6N2E2_5771 +0.5 3.3 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_145 +0.5 2.5 putative exported protein compare
Pf6N2E2_377 +0.5 1.3 hypothetical protein compare
Pf6N2E2_1514 +0.5 2.3 lipoprotein OprI, putative compare
Pf6N2E2_1312 +0.5 1.5 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS) compare
Pf6N2E2_919 +0.5 1.3 FIG00954373: hypothetical protein compare
Pf6N2E2_2628 +0.5 2.2 Flagellar basal-body rod protein FlgF compare
Pf6N2E2_2688 +0.5 1.5 YD repeat protein compare
Pf6N2E2_2795 +0.5 1.4 putative exported protein compare
Pf6N2E2_2390 +0.5 2.0 FIG00959101: hypothetical protein compare
Pf6N2E2_3899 +0.5 3.2 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_1825 +0.5 2.0 FOG: EAL domain protein compare
Pf6N2E2_4365 +0.5 3.7 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_3226 +0.5 1.6 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_5768 +0.5 2.1 DNA recombination-dependent growth factor C compare
Pf6N2E2_1839 +0.5 1.2 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-) compare
Pf6N2E2_6018 +0.5 1.8 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_1024 +0.5 1.9 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases compare
Pf6N2E2_4556 +0.5 2.2 FIG00953078: hypothetical protein compare
Pf6N2E2_5622 +0.5 1.9 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_2734 +0.5 2.2 Transcriptional regulator, LysR family compare
Pf6N2E2_5394 +0.5 1.6 Molybdenum cofactor biosynthesis protein MoaE compare
Pf6N2E2_2908 +0.5 2.2 Arginine deiminase (EC 3.5.3.6) compare
Pf6N2E2_6040 +0.5 1.4 hypothetical protein compare
Pf6N2E2_6030 +0.5 2.3 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_2459 +0.5 1.1 Organic hydroperoxide resistance protein compare
Pf6N2E2_1032 +0.5 0.7 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_1031 +0.5 1.8 Alkanesulfonates transport system permease protein compare
Pf6N2E2_4827 +0.5 2.5 Lipoate-protein ligase A compare
Pf6N2E2_2549 +0.5 0.9 hypothetical protein compare
Pf6N2E2_4340 +0.5 2.3 Outer membrane protein ImpK/VasF, OmpA/MotB domain compare
Pf6N2E2_4963 +0.5 0.8 Homoserine dehydrogenase (EC 1.1.1.3) compare
Pf6N2E2_2518 +0.5 0.9 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_2188 +0.5 1.6 Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family compare
Pf6N2E2_1834 +0.5 1.1 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_3763 +0.5 1.4 hypothetical protein compare
Pf6N2E2_1347 +0.5 1.5 Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-) compare
Pf6N2E2_4172 +0.5 2.6 Methionine ABC transporter ATP-binding protein compare
Pf6N2E2_339 +0.5 1.4 Glutathione-regulated potassium-efflux system protein KefC compare
Pf6N2E2_3770 +0.5 1.4 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_4728 +0.5 2.3 hypothetical protein compare
Pf6N2E2_2288 +0.5 1.9 FIG00953423: hypothetical protein compare
Pf6N2E2_3144 +0.5 2.5 Thiol:disulfide interchange protein DsbC compare
Pf6N2E2_1273 +0.5 1.5 Probable RND efflux membrane fusion protein compare
Pf6N2E2_374 +0.4 1.8 FIG00953249: hypothetical protein compare
Pf6N2E2_2083 +0.4 1.9 hypothetical protein compare
Pf6N2E2_5499 +0.4 0.9 hypothetical protein compare
Pf6N2E2_3139 +0.4 2.6 L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16) compare
Pf6N2E2_2047 +0.4 1.9 D-glucosaminate dehydratase (EC 4.3.1.9) (from data) compare
Pf6N2E2_4381 +0.4 1.7 hypothetical protein compare
Pf6N2E2_3088 +0.4 1.1 hypothetical protein compare
Pf6N2E2_2911 +0.4 2.9 Acetyl-CoA carboxylase alpha subunit compare
Pf6N2E2_1516 +0.4 1.2 transcriptional regulator, Crp/Fnr family compare
Pf6N2E2_2111 +0.4 1.6 Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit A (from data) compare
Pf6N2E2_3606 +0.4 3.3 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-) compare
Pf6N2E2_2003 +0.4 1.4 hypothetical protein compare
Pf6N2E2_841 +0.4 1.4 hypothetical protein compare
Pf6N2E2_1803 +0.4 1.8 Polysaccharide deacetylase compare
Pf6N2E2_1156 +0.4 1.6 hypothetical protein compare
Pf6N2E2_5052 +0.4 1.2 Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family compare
Pf6N2E2_726 +0.4 1.7 Nitrate/nitrite sensor protein (EC 2.7.3.-) compare
Pf6N2E2_98 +0.4 0.8 putative transmembrane protein compare
Pf6N2E2_1536 +0.4 2.3 Permeases of the major facilitator superfamily compare
Pf6N2E2_1470 +0.4 1.4 Transcriptional regulator, LysR family compare
Pf6N2E2_1392 +0.4 2.0 sensor histidine kinase compare
Pf6N2E2_2181 +0.4 0.9 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_5814 +0.4 3.0 Glutaredoxin compare
Pf6N2E2_1199 +0.4 1.7 hypothetical protein compare
Pf6N2E2_1726 +0.4 2.6 3-methylmercaptopropionyl-CoA ligase (DmdB) compare
Pf6N2E2_64 +0.4 1.2 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_2285 +0.4 0.8 FIG00954079: hypothetical protein compare
Pf6N2E2_2103 +0.4 0.8 hypothetical protein compare
Pf6N2E2_5018 +0.4 2.7 tRNA-i(6)A37 methylthiotransferase compare
Pf6N2E2_6064 +0.4 1.9 2-methylaconitate isomerase compare
Pf6N2E2_1476 +0.4 1.2 (AF179595) Vco33 compare
Pf6N2E2_4047 +0.4 2.1 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_3338 +0.4 0.7 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_3007 +0.4 2.2 Phage tail length tape-measure protein compare
Pf6N2E2_1984 +0.4 1.3 probable exported protein STY4558 compare
Pf6N2E2_3521 +0.4 3.0 Sensor histidine kinase/response regulator compare
Pf6N2E2_5455 +0.4 1.6 hypothetical protein compare
Pf6N2E2_2148 +0.4 1.5 Glutathione S-transferase (EC 2.5.1.18) compare
Pf6N2E2_1301 +0.4 1.8 TRAP-type C4-dicarboxylate transport system, periplasmic component compare
Pf6N2E2_4054 +0.4 1.6 Osmotically inducible protein C compare
Pf6N2E2_4581 +0.4 2.6 FIG015547: peptidase, M16 family compare
Pf6N2E2_5695 +0.4 2.3 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_1239 +0.4 2.2 Putative TEGT family carrier/transport protein compare
Pf6N2E2_373 +0.4 2.0 Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) compare
Pf6N2E2_5545 +0.4 1.7 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_1434 +0.4 1.2 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_3002 +0.4 2.7 Phage FluMu protein gp47 compare
Pf6N2E2_5130 +0.4 1.5 hypothetical protein compare
Pf6N2E2_2754 +0.4 1.5 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf6N2E2_672 +0.4 1.2 ABC-type polar amino acid transport system, ATPase component compare
Pf6N2E2_3941 +0.4 1.6 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_2399 +0.4 2.4 Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase compare
Pf6N2E2_4804 +0.4 1.9 ApaG protein compare
Pf6N2E2_264 +0.4 1.2 Transcriptional regulator, TetR family compare
Pf6N2E2_5030 +0.4 2.5 Transcriptional regulator, AsnC family compare


Specific Phenotypes

For 6 genes in this experiment

For stress Benzethonium chloride in Pseudomonas fluorescens FW300-N2E2

For stress Benzethonium chloride across organisms