Experiment set2IT039 for Pseudomonas fluorescens FW300-N2E2
LB with Benzethonium chloride 0.02 mM
Group: stressMedia: LB + Benzethonium chloride (0.02 mM)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1183 A3,A4
Specific Phenotypes
For 6 genes in this experiment
For stress Benzethonium chloride in Pseudomonas fluorescens FW300-N2E2
For stress Benzethonium chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 1 |
| Cobalt-zinc-cadmium resistance | 1 |
| Hemin transport system | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| choline degradation I | 2 | 2 | 1 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| choline-O-sulfate degradation | 3 | 3 | 1 |