Experiment set2IT039 for Pseudomonas fluorescens FW300-N1B4

LB with Cisplatin 0.006 mg/ml

Group: stress
Media: LB + Cisplatin (0.006 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Jayashree on 4/23/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 889 E5,E6

Specific Phenotypes

For 13 genes in this experiment

For stress Cisplatin in Pseudomonas fluorescens FW300-N1B4

For stress Cisplatin across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	1
DNA repair, UvrABC system	1
DNA repair, bacterial DinG and relatives	1
DNA repair, bacterial RecFOR pathway	1
Peptidoglycan Biosynthesis	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan recycling II	10	9	1
peptidoglycan recycling I	14	11	1