Experiment set2IT039 for Shewanella sp. ANA-3

LB with Paraquat dichloride 0.008 mg/ml

Group: stress
Media: LB + Paraquat dichloride (0.008 mg/ml)
Culturing: ANA3_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/15/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 923 D1,D2

Specific Phenotypes

For 15 genes in this experiment

For stress Paraquat dichloride in Shewanella sp. ANA-3

For stress Paraquat dichloride across organisms

SEED Subsystems

Subsystem	#Specific
Biogenesis of cbb3-type cytochrome c oxidases	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
DNA repair, bacterial MutL-MutS system	1
Experimental tye	1
Heme and Siroheme Biosynthesis	1
Multidrug Resistance Efflux Pumps	1
Terminal cytochrome C oxidases	1
Terminal cytochrome O ubiquinol oxidase	1
Terminal cytochrome oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Ubiquinone and menaquinone biosynthesis
• Methionine metabolism
• Tryptophan metabolism
• Riboflavin metabolism
• Terpenoid biosynthesis
• Carotenoid biosynthesis - General
• Biosynthesis of terpenoids and steroids
• mTOR signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tetrapyrrole biosynthesis II (from glycine)	4	3	1
heme a biosynthesis	4	2	1
tetrapyrrole biosynthesis I (from glutamate)	6	6	1
superpathway of heme b biosynthesis from glycine	8	7	1
superpathway of heme b biosynthesis from glutamate	10	10	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	1
adenosylcobalamin biosynthesis II (aerobic)	33	18	1
adenosylcobalamin biosynthesis I (anaerobic)	36	18	1