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Experiment set2IT038 for
Methanococcus maripaludis JJ
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H2 McCas, 5g/L bicarb, 40 psi H2CO2
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS04415
+2.6
9.9
hypothetical protein
compare
MMJJ
_RS04720
+1.6
2.0
IMP dehydrogenase
compare
MMJJ
_RS05765
+1.0
1.6
flagellar protein E
compare
MMJJ
_RS08450
+0.9
6.7
undecaprenyl-diphosphate phosphatase
compare
MMJJ
_RS03205
+0.8
5.4
metallophosphoesterase
compare
MMJJ
_RS03360
+0.8
1.6
hypothetical protein
compare
MMJJ
_RS04580
+0.7
5.7
2-phosphosulfolactate phosphatase
compare
MMJJ
_RS01100
+0.7
1.6
helix-turn-helix domain-containing protein
compare
MMJJ
_RS08830
+0.7
3.0
hypothetical protein
compare
MMJJ
_RS05930
+0.7
1.1
cell division protein SepF
compare
MMJJ
_RS04575
+0.7
4.0
DUF116 domain-containing protein
compare
MMJJ
_RS04215
+0.7
6.0
DMT family transporter
compare
MMJJ
_RS00415
+0.6
3.0
DedA family protein
compare
MMJJ
_RS00035
+0.6
5.3
CO dehydrogenase/acetyl-CoA synthase complex subunit alpha
compare
MMJJ
_RS04935
+0.6
2.0
4Fe-4S binding protein
compare
MMJJ
_RS04360
+0.6
4.1
winged helix-turn-helix transcriptional regulator
compare
MMJJ
_RS07035
+0.6
1.0
glycosyl transferase
compare
MMJJ
_RS00045
+0.6
4.5
CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta
compare
MMJJ
_RS07040
+0.5
0.7
preprotein translocase subunit SecY
compare
MMJJ
_RS00040
+0.5
2.8
CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon
compare
MMJJ
_RS04480
+0.5
2.2
hypothetical protein
compare
MMJJ
_RS00050
+0.4
3.1
AAA family ATPase
compare
MMJJ
_RS01785
+0.4
0.9
50S ribosomal protein L14e
compare
MMJJ
_RS05770
+0.4
1.0
flagellar protein D
compare
MMJJ
_RS06940
+0.4
3.0
metalloregulator ArsR/SmtB family transcription factor
compare
MMJJ
_RS06055
+0.4
3.4
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMJJ
_RS01060
+0.4
2.1
ABC transporter ATP-binding protein
compare
MMJJ
_RS00055
+0.4
2.6
CO dehydrogenase/acetyl-CoA synthase subunit delta
compare
MMJJ
_RS07230
+0.4
3.2
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS07235
+0.4
3.6
coenzyme F420 hydrogenase subunit alpha
compare
MMJJ
_RS06210
+0.4
2.8
hypothetical protein
compare
MMJJ
_RS00480
+0.4
3.3
flippase
compare
MMJJ
_RS00525
+0.4
1.6
Dabb family protein
compare
MMJJ
_RS07225
+0.4
2.7
coenzyme F420 hydrogenase subunit gamma
compare
MMJJ
_RS09255
+0.4
1.6
hypothetical protein
compare
MMJJ
_RS06130
+0.4
2.0
hypothetical protein
compare
MMJJ
_RS09390
+0.4
1.4
hypothetical protein
compare
MMJJ
_RS06535
+0.3
3.0
UPF0104 family protein
compare
MMJJ
_RS06810
+0.3
3.0
L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
compare
MMJJ
_RS07590
+0.3
2.6
flap endonuclease-1
compare
MMJJ
_RS03655
+0.3
1.4
replication factor C large subunit
compare
MMJJ
_RS08935
+0.3
0.7
TIGR00288 family NYN domain-containing protein
compare
MMJJ
_RS06115
+0.3
2.7
pyruvate kinase
compare
MMJJ
_RS04780
+0.3
1.2
nucleic acid-binding protein
compare
MMJJ
_RS07220
+0.3
2.8
coenzyme F420 hydrogenase subunit beta
compare
MMJJ
_RS03430
+0.3
1.7
winged helix-turn-helix domain-containing protein
compare
MMJJ
_RS01125
+0.3
2.1
hypothetical protein
compare
MMJJ
_RS08380
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS06380
+0.3
1.0
signal recognition particle protein Srp54
compare
MMJJ
_RS07485
+0.3
2.2
DedA family protein
compare
MMJJ
_RS03605
+0.3
1.6
TIGR00297 family protein
compare
MMJJ
_RS07660
+0.3
1.8
carbonic anhydrase
compare
MMJJ
_RS00510
+0.3
2.5
DEAD/DEAH box helicase
compare
MMJJ
_RS03410
+0.3
1.0
hypothetical protein
compare
MMJJ
_RS08730
+0.3
2.4
glycosyltransferase family 4 protein
compare
MMJJ
_RS05970
+0.3
1.2
MJ0307 family thioredoxin
compare
MMJJ
_RS01035
+0.3
1.6
hypothetical protein
compare
MMJJ
_RS06755
+0.3
2.3
diadenylate cyclase
compare
MMJJ
_RS06585
+0.3
2.2
alanine racemase
compare
MMJJ
_RS00060
+0.3
2.3
acetyl-CoA decarbonylase/synthase complex subunit gamma
compare
MMJJ
_RS09365
+0.3
1.4
hypothetical protein
compare
MMJJ
_RS00830
+0.3
2.1
corrinoid protein
compare
MMJJ
_RS09325
+0.3
1.7
hypothetical protein
compare
MMJJ
_RS09370
+0.3
1.0
heavy-metal-associated domain-containing protein
compare
MMJJ
_RS07630
+0.2
1.8
hypothetical protein
compare
MMJJ
_RS06495
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS06680
+0.2
1.7
DUF366 family protein
compare
MMJJ
_RS07835
+0.2
1.8
UPF0489 family protein
compare
MMJJ
_RS09380
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS04055
+0.2
1.3
hypothetical protein
compare
MMJJ
_RS06465
+0.2
1.6
hypothetical protein
compare
MMJJ
_RS00420
+0.2
1.5
CBS domain-containing protein
compare
MMJJ
_RS08805
+0.2
1.5
deoxyuridine 5'-triphosphate nucleotidohydrolase
compare
MMJJ
_RS08165
+0.2
1.4
thermonuclease family protein
compare
MMJJ
_RS06085
+0.2
1.5
proteasome assembly chaperone family protein
compare
MMJJ
_RS08930
+0.2
1.7
5'/3'-nucleotidase SurE
compare
MMJJ
_RS01825
+0.2
1.4
DNA repair and recombination protein RadB
compare
MMJJ
_RS07665
+0.2
2.0
formate dehydrogenase subunit alpha
compare
MMJJ
_RS07825
+0.2
1.0
YciI family protein
compare
MMJJ
_RS06440
+0.2
1.7
DUF4870 domain-containing protein
compare
MMJJ
_RS06560
+0.2
1.7
site-2 protease family protein
compare
MMJJ
_RS09265
+0.2
0.9
hypothetical protein
compare
MMJJ
_RS01010
+0.2
2.0
right-handed parallel beta-helix repeat-containing protein
compare
MMJJ
_RS01405
+0.2
0.4
proline--tRNA ligase
compare
MMJJ
_RS01950
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS07690
+0.2
1.6
glycosyltransferase family 4 protein
compare
MMJJ
_RS06765
+0.2
1.8
hypothetical protein
compare
MMJJ
_RS04175
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS06550
+0.2
1.8
ABC transporter permease
compare
MMJJ
_RS03915
+0.2
1.3
hypothetical protein
compare
MMJJ
_RS06445
+0.2
1.8
hypothetical protein
compare
MMJJ
_RS06770
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS03690
+0.2
1.2
indolepyruvate oxidoreductase subunit beta
compare
MMJJ
_RS06105
+0.2
1.6
DUF99 family protein
compare
MMJJ
_RS01955
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS06470
+0.2
1.5
tripartite tricarboxylate transporter permease
compare
MMJJ
_RS00460
+0.2
1.0
selenouridine synthase SelU-like subunit
compare
MMJJ
_RS08740
+0.2
1.7
class I SAM-dependent methyltransferase
compare
MMJJ
_RS05190
+0.2
1.2
gamma carbonic anhydrase family protein
compare
MMJJ
_RS01515
+0.2
1.2
DUF2178 domain-containing protein
compare
MMJJ
_RS01615
+0.2
1.2
MoaD/ThiS family protein
compare
MMJJ
_RS06520
+0.2
1.4
TldD/PmbA family protein
compare
MMJJ
_RS01275
+0.2
1.2
4Fe-4S dicluster domain-containing protein
compare
MMJJ
_RS01920
+0.2
1.6
phosphoglycerate mutase
compare
MMJJ
_RS08075
+0.2
1.5
ATP-dependent DNA helicase
compare
MMJJ
_RS07610
+0.2
1.4
DUF2119 domain-containing protein
compare
MMJJ
_RS07655
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS06275
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS00785
+0.2
0.5
hypothetical protein
compare
MMJJ
_RS04135
+0.2
1.2
7-cyano-7-deazaguanine synthase QueC
compare
MMJJ
_RS05680
+0.2
1.6
hypothetical protein
compare
MMJJ
_RS08400
+0.2
1.1
CoB--CoM heterodisulfide reductase subunit C
compare
MMJJ
_RS07595
+0.2
0.8
hypothetical protein
compare
MMJJ
_RS02665
+0.2
0.5
methyl-accepting chemotaxis protein
compare
MMJJ
_RS09155
+0.2
1.4
RluA family pseudouridine synthase
compare
MMJJ
_RS03725
+0.2
0.6
sulfurtransferase TusA family protein
compare
MMJJ
_RS07215
+0.2
1.4
TIGR00341 family protein
compare
MMJJ
_RS01290
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS05695
+0.2
1.2
class III signal peptide-containing protein
compare
MMJJ
_RS04810
+0.2
1.2
nitroreductase family protein
compare
MMJJ
_RS09145
+0.2
1.1
exodeoxyribonuclease III
compare
MMJJ
_RS08345
+0.2
1.2
flavodoxin family protein
compare
MMJJ
_RS09200
+0.2
1.3
Nre family DNA repair protein
compare
MMJJ
_RS01960
+0.2
0.7
glycosyltransferase family 2 protein
compare
MMJJ
_RS09330
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS05710
+0.2
0.6
single-stranded-DNA-specific exonuclease RecJ
compare
MMJJ
_RS08040
+0.2
1.3
NAD(P)/FAD-dependent oxidoreductase
compare
MMJJ
_RS03440
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS07645
+0.1
1.1
selenium-binding protein
compare
MMJJ
_RS07795
+0.1
1.0
cupin domain-containing protein
compare
MMJJ
_RS06720
+0.1
1.3
mechanosensitive ion channel family protein
compare
MMJJ
_RS06430
+0.1
1.1
haloacid dehalogenase
compare
MMJJ
_RS05885
+0.1
1.3
tungstate ABC transporter substrate-binding protein WtpA
compare
MMJJ
_RS06555
+0.1
1.2
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS03070
+0.1
0.4
MetS family NSS transporter small subunit
compare
MMJJ
_RS06280
+0.1
1.2
hypothetical protein
compare
MMJJ
_RS07450
+0.1
1.1
hypothetical protein
compare
MMJJ
_RS05290
+0.1
1.3
DUF4013 domain-containing protein
compare
MMJJ
_RS05985
+0.1
1.1
ABC transporter substrate-binding protein
compare
MMJJ
_RS08385
+0.1
1.1
class II SORL domain-containing protein
compare
MMJJ
_RS06120
+0.1
1.1
DUF4013 domain-containing protein
compare
MMJJ
_RS07210
+0.1
1.2
AmmeMemoRadiSam system protein B
compare
MMJJ
_RS07910
+0.1
1.1
CBS domain-containing protein
compare
MMJJ
_RS06580
+0.1
1.2
alanine dehydrogenase
compare
MMJJ
_RS02245
+0.1
1.1
DUF432 domain-containing protein
compare
MMJJ
_RS01990
+0.1
0.7
universal stress protein
compare
MMJJ
_RS01285
+0.1
0.9
CopG family ribbon-helix-helix protein
compare
MMJJ
_RS01250
+0.1
0.8
hypothetical protein
compare
MMJJ
_RS08675
+0.1
1.0
phosphate ABC transporter ATP-binding protein PstB
compare
MMJJ
_RS08460
+0.1
1.1
metallophosphoesterase
compare
MMJJ
_RS05750
+0.1
1.1
flagellar accessory protein FlaH
compare
MMJJ
_RS01230
+0.1
1.0
EamA family transporter
compare
MMJJ
_RS05975
+0.1
1.1
sulfurtransferase TusA family protein
compare
MMJJ
_RS07480
+0.1
1.2
FAD-dependent oxidoreductase
compare
MMJJ
_RS07635
+0.1
1.0
response regulator transcription factor
compare
MMJJ
_RS08690
+0.1
1.0
phosphate ABC transporter substrate-binding protein
compare
MMJJ
_RS03080
+0.1
1.0
carbohydrate kinase family protein
compare
MMJJ
_RS04465
+0.1
1.0
MTAP family purine nucleoside phosphorylase
compare
MMJJ
_RS07025
+0.1
0.8
tRNA (cytidine(56)-2'-O)-methyltransferase
compare
MMJJ
_RS03715
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS07385
+0.1
1.0
ribosomal protein S18-alanine N-acetyltransferase
compare
MMJJ
_RS05745
+0.1
1.0
type II/IV secretion system ATPase subunit
compare
MMJJ
_RS03185
+0.1
0.3
alanine--tRNA ligase
compare
MMJJ
_RS02125
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS09060
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS02320
+0.1
0.8
VWA domain-containing protein
compare
MMJJ
_RS00070
+0.1
0.5
CooT family nickel-binding protein
compare
MMJJ
_RS07760
+0.1
0.9
GNAT family N-acetyltransferase
compare
MMJJ
_RS03370
+0.1
0.5
hypothetical protein
compare
MMJJ
_RS08160
+0.1
0.8
tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
compare
MMJJ
_RS09105
+0.1
1.0
AI-2E family transporter
compare
MMJJ
_RS06960
+0.1
1.1
CAP domain-containing protein
compare
MMJJ
_RS05510
+0.1
0.9
archaellum operon transcriptional activator EarA family protein
compare
MMJJ
_RS05250
+0.1
0.8
HDIG domain-containing protein
compare
MMJJ
_RS03805
+0.1
0.4
hypothetical protein
compare
MMJJ
_RS04375
+0.1
0.3
hypothetical protein
compare
MMJJ
_RS05505
+0.1
1.1
hypothetical protein
compare
MMJJ
_RS07365
+0.1
1.0
CatB-related O-acetyltransferase
compare
MMJJ
_RS01770
+0.1
0.7
TOBE domain-containing protein
compare
MMJJ
_RS04765
+0.1
0.8
metal-dependent hydrolase
compare
MMJJ
_RS05740
+0.1
1.0
archaellar assembly protein FlaJ
compare
MMJJ
_RS02885
+0.1
0.8
NifB/NifX family molybdenum-iron cluster-binding protein
compare
MMJJ
_RS00540
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS08370
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS06800
+0.1
0.8
prefoldin subunit alpha
compare
MMJJ
_RS08880
+0.1
0.9
IMPACT family protein
compare
MMJJ
_RS05730
+0.1
1.0
TIM barrel protein
compare
MMJJ
_RS08885
+0.1
0.9
cysteine--tRNA ligase
compare
MMJJ
_RS00675
+0.1
0.9
nitrogenase
compare
MMJJ
_RS05760
+0.1
1.0
flagellar protein F
compare
MMJJ
_RS05940
+0.1
0.9
HAD family hydrolase
compare
MMJJ
_RS03045
+0.1
0.8
hypothetical protein
compare
MMJJ
_RS08175
+0.1
0.7
winged helix-turn-helix transcriptional regulator
compare
MMJJ
_RS07865
+0.1
0.8
zinc ribbon domain-containing protein
compare
MMJJ
_RS07255
+0.1
1.0
hypothetical protein
compare
MMJJ
_RS06710
+0.1
1.0
TldD/PmbA family protein
compare
MMJJ
_RS04300
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS06100
+0.1
0.9
rubrerythrin
compare
MMJJ
_RS06640
+0.1
0.8
YfcE family phosphodiesterase
compare
MMJJ
_RS08025
+0.1
0.4
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in rich t1 experiments