Experiment set2IT036 for Rhodanobacter denitrificans FW104-10B01

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R2A with Sodium arsenate dibasic heptahydrate 15.625 mM

Group: stress
Media: R2A + Sodium arsenate dibasic heptahydrate (15.625 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 02/27/2021
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 10 genes in this experiment

For stress Sodium arsenate dibasic heptahydrate in Rhodanobacter denitrificans FW104-10B01

For stress Sodium arsenate dibasic heptahydrate across organisms

SEED Subsystems

Subsystem #Specific
Arsenic resistance 2
Calvin-Benson cycle 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Lipoic acid metabolism 1
RNA processing and degradation, bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
lipoate biosynthesis and incorporation I 2 2 1
lipoate biosynthesis and incorporation V (mammals) 3 2 1
lipoate biosynthesis and incorporation III (Bacillus) 3 2 1
tRNA processing 10 10 2
lipoate biosynthesis and incorporation IV (yeast) 7 4 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
glycolysis II (from fructose 6-phosphate) 11 11 1
gluconeogenesis III 12 10 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
glycolysis I (from glucose 6-phosphate) 13 12 1
gluconeogenesis I 13 11 1
Calvin-Benson-Bassham cycle 13 9 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 16 1
oxygenic photosynthesis 17 10 1
superpathway of hexitol degradation (bacteria) 18 14 1
superpathway of anaerobic sucrose degradation 19 16 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 16 1
ethene biosynthesis V (engineered) 25 17 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 1
1-butanol autotrophic biosynthesis (engineered) 27 18 1