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Experiment set2IT033 for
Methanococcus maripaludis S2
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Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS05975
+1.1
2.5
hypothetical protein
compare
MMP
_RS09025
+0.9
1.6
hypothetical protein
compare
MMP
_RS02595
+0.9
1.7
putative zinc-binding protein
compare
MMP
_RS07970
+0.8
3.4
hypothetical protein
compare
MMP
_RS06975
+0.8
1.8
sulfide-dependent adenosine diphosphate thiazole synthase
compare
MMP
_RS07125
+0.8
3.9
coenzyme F420-reducing hydrogenase FrhD protein
compare
MMP
_RS07860
+0.8
0.6
hypothetical protein
compare
MMP
_RS01650
+0.8
1.0
sulfurtransferase TusA family protein
compare
MMP
_RS03300
+0.7
0.7
TOBE domain-containing protein
compare
MMP
_RS06545
+0.7
3.7
ferredoxin family protein
compare
MMP
_RS01260
+0.7
2.3
HDIG domain-containing protein
compare
MMP
_RS00675
+0.7
0.8
nitroreductase family protein
compare
MMP
_RS06540
+0.7
2.4
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMP
_RS03450
+0.7
2.2
glycine synthesis protein GlyXS
(from data)
compare
MMP
_RS08775
+0.6
2.7
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS02045
+0.6
1.5
tRNA-Met
compare
MMP
_RS06905
+0.6
1.5
hypothetical protein
compare
MMP
_RS02790
+0.6
1.1
class III signal peptide-containing protein
compare
MMP
_RS01115
+0.6
4.6
molybdopterin molybdotransferase MoeA
compare
MMP
_RS07840
+0.6
2.2
ribonuclease III
compare
MMP
_RS01435
+0.6
2.0
DMT family transporter
compare
MMP
_RS05970
+0.5
1.7
class II SORL domain-containing protein
compare
MMP
_RS06190
+0.5
2.5
tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
compare
MMP
_RS07345
+0.5
2.1
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS07120
+0.5
3.3
coenzyme F420 hydrogenase subunit alpha
compare
MMP
_RS06485
+0.5
0.8
zinc ribbon domain-containing protein
compare
MMP
_RS03440
+0.5
0.9
MoaD/ThiS family protein
compare
MMP
_RS02370
+0.5
1.9
NifB/NifX family molybdenum-iron cluster-binding protein
compare
MMP
_RS07560
+0.5
2.3
prefoldin subunit alpha
compare
MMP
_RS08500
+0.5
2.4
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMP
_RS03520
+0.5
1.4
YwbE family protein
compare
MMP
_RS03340
+0.5
0.6
ABC transporter ATP-binding protein
compare
MMP
_RS09050
+0.5
2.3
CAP domain-containing protein
compare
MMP
_RS06590
+0.5
1.1
GNAT family N-acetyltransferase
compare
MMP
_RS08455
+0.5
2.1
multiprotein bridging factor aMBF1
compare
MMP
_RS01150
+0.5
1.6
aldo/keto reductase
compare
MMP
_RS08615
+0.5
1.1
flagellar protein G
compare
MMP
_RS06610
+0.5
1.7
DUF2334 domain-containing protein
compare
MMP
_RS01055
+0.5
1.5
hypothetical protein
compare
MMP
_RS07130
+0.5
2.4
coenzyme F420 hydrogenase subunit gamma
compare
MMP
_RS02605
+0.5
0.5
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS00540
+0.4
2.5
PLP-dependent aminotransferase family protein
compare
MMP
_RS06520
+0.4
1.4
DUF1786 domain-containing protein
compare
MMP
_RS08380
+0.4
2.6
phosphatase PAP2 family protein
compare
MMP
_RS00640
+0.4
2.2
ABC transporter substrate-binding protein
compare
MMP
_RS08830
+0.4
2.1
GTP-binding protein
compare
MMP
_RS01370
+0.4
2.8
acetate--CoA ligase family protein
compare
MMP
_RS08265
+0.4
2.3
rubrerythrin
compare
MMP
_RS07915
+0.4
1.6
DUF4870 domain-containing protein
compare
MMP
_RS06585
+0.4
1.0
DMT family protein
compare
MMP
_RS05780
+0.4
3.0
multidrug resistance efflux transporter family protein
compare
MMP
_RS03965
+0.4
1.3
hypothetical protein
compare
MMP
_RS07550
+0.4
2.6
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS06335
+0.4
2.2
HAD family hydrolase
compare
MMP
_RS06300
+0.4
2.7
ABC transporter substrate-binding protein
compare
MMP
_RS08205
+0.4
2.6
stage II sporulation protein M
compare
MMP
_RS08700
+0.4
2.8
hypothetical protein
compare
MMP
_RS07900
+0.4
1.0
YeeE/YedE family protein
compare
MMP
_RS07135
+0.4
2.3
coenzyme F420 hydrogenase subunit beta
compare
MMP
_RS07895
+0.4
1.3
cytochrome c
compare
MMP
_RS07985
+0.4
3.1
nitroreductase family protein
compare
MMP
_RS05110
+0.4
2.6
16S ribosomal RNA methyltransferase A
compare
MMP
_RS01460
+0.4
1.6
hypothetical protein
compare
MMP
_RS08485
+0.4
2.7
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS08275
+0.4
2.6
hypothetical protein
compare
MMP
_RS00125
+0.4
0.8
DUF2798 domain-containing protein
compare
MMP
_RS08415
+0.4
1.4
radical SAM protein
compare
MMP
_RS08835
+0.4
2.2
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS05140
+0.4
1.8
helix-turn-helix transcriptional regulator
compare
MMP
_RS01275
+0.4
2.5
class III signal peptide-containing protein
compare
MMP
_RS07805
+0.4
2.0
ABC transporter permease
compare
MMP
_RS02310
+0.4
0.9
RimK-like ATPgrasp N-terminal domain-containing protein
compare
MMP
_RS04100
+0.4
1.1
hypothetical protein
compare
MMP
_RS00545
+0.4
1.6
XRE family transcriptional regulator
compare
MMP
_RS06185
+0.4
1.6
thermonuclease family protein
compare
MMP
_RS00990
+0.4
2.1
hypothetical protein
compare
MMP
_RS07650
+0.4
2.8
TldD/PmbA family protein
compare
MMP
_RS02745
+0.4
1.1
hypothetical protein
compare
MMP
_RS00950
+0.4
2.1
site-2 protease family protein
compare
MMP
_RS01570
+0.4
0.7
hypothetical protein
compare
MMP
_RS01785
+0.4
2.3
hypothetical protein
compare
MMP
_RS07145
+0.4
1.8
AmmeMemoRadiSam system protein B
compare
MMP
_RS06165
+0.3
2.6
ABC transporter permease
compare
MMP
_RS08930
+0.3
0.8
hypothetical protein
compare
MMP
_RS06430
+0.3
1.5
6-carboxytetrahydropterin synthase QueD
compare
MMP
_RS00635
+0.3
1.6
energy-coupling factor transporter transmembrane protein EcfT
compare
MMP
_RS08430
+0.3
1.8
HAD family hydrolase
compare
MMP
_RS01990
+0.3
1.4
hypothetical protein
compare
MMP
_RS05865
+0.3
1.3
hypothetical protein
compare
MMP
_RS06665
+0.3
2.3
glycogen/starch synthase
compare
MMP
_RS08480
+0.3
1.9
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS01485
+0.3
2.0
right-handed parallel beta-helix repeat-containing protein
compare
MMP
_RS07115
+0.3
1.9
MBL fold metallo-hydrolase
compare
MMP
_RS06965
+0.3
1.6
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS02500
+0.3
1.7
minichromosome maintenance protein MCM
compare
MMP
_RS01420
+0.3
0.8
hypothetical protein
compare
MMP
_RS06005
+0.3
1.7
heavy-metal-associated domain-containing protein
compare
MMP
_RS03215
+0.3
2.2
MBL fold metallo-hydrolase
compare
MMP
_RS00380
+0.3
1.4
P-II family nitrogen regulator
compare
MMP
_RS06525
+0.3
0.5
YciI family protein
compare
MMP
_RS05535
+0.3
1.9
hypothetical protein
compare
MMP
_RS01940
+0.3
1.1
MoxR family ATPase
compare
MMP
_RS07770
+0.3
2.0
alanine racemase
compare
MMP
_RS05720
+0.3
1.3
signal recognition particle subunit SRP19/SEC65 family protein
compare
MMP
_RS07100
+0.3
2.2
hypothetical protein
compare
MMP
_RS07185
+0.3
2.1
pyridoxal phosphate-dependent aminotransferase
compare
MMP
_RS08390
+0.3
1.7
DUF5400 domain-containing protein
compare
MMP
_RS07775
+0.3
1.4
alanine dehydrogenase
compare
MMP
_RS06765
+0.3
1.5
flap endonuclease-1
compare
MMP
_RS08280
+0.3
1.3
proteasome assembly chaperone family protein
compare
MMP
_RS06440
+0.3
1.4
CBS domain-containing protein
compare
MMP
_RS02490
+0.3
1.0
hypothetical protein
compare
MMP
_RS01430
+0.3
1.6
mechanosensitive ion channel family protein
compare
MMP
_RS00395
+0.3
0.6
MGMT family protein
compare
MMP
_RS06910
+0.3
1.6
RlmF-related methyltransferase
compare
MMP
_RS06045
+0.3
1.8
DNA polymerase
compare
MMP
_RS02320
+0.3
2.0
glutamate-cysteine ligase family protein
compare
MMP
_RS02265
+0.3
1.5
nitroreductase family protein
compare
MMP
_RS01065
+0.3
2.2
alpha/beta hydrolase
compare
MMP
_RS06465
+0.3
2.0
NERD domain-containing protein
compare
MMP
_RS07590
+0.3
1.8
hypothetical protein
compare
MMP
_RS07925
+0.3
1.2
haloacid dehalogenase
compare
MMP
_RS08080
+0.3
1.6
YchF/TatD family DNA exonuclease
compare
MMP
_RS08155
+0.3
1.8
hypothetical protein
compare
MMP
_RS02300
+0.3
1.4
NAD(P)H-dependent oxidoreductase
compare
MMP
_RS06710
+0.3
1.2
selenium-binding protein
compare
MMP
_RS02770
+0.3
1.1
hypothetical protein
compare
MMP
_RS04530
+0.3
1.2
ABC transporter permease
compare
MMP
_RS00630
+0.3
1.8
ECF transporter S component
compare
MMP
_RS04300
+0.3
1.5
hypothetical protein
compare
MMP
_RS06990
+0.3
1.4
CatB-related O-acetyltransferase
compare
MMP
_RS00155
+0.2
1.1
heavy metal-binding domain-containing protein
compare
MMP
_RS06655
+0.2
1.4
glycoside hydrolase family 15 protein
compare
MMP
_RS07155
+0.2
1.7
MFS transporter
compare
MMP
_RS02380
+0.2
1.1
ATP-binding protein
compare
MMP
_RS08160
+0.2
1.4
EamA family transporter
compare
MMP
_RS08400
+0.2
1.1
MJ0307 family thioredoxin
compare
MMP
_RS06930
+0.2
1.6
MBL fold metallo-hydrolase
compare
MMP
_RS00435
+0.2
1.7
CPBP family intramembrane metalloprotease
compare
MMP
_RS04165
+0.2
0.9
TetR/AcrR family transcriptional regulator
compare
MMP
_RS01125
+0.2
1.1
thioredoxin family protein
compare
MMP
_RS00080
+0.2
0.9
RecB-family nuclease
compare
MMP
_RS00955
+0.2
1.6
coenzyme gamma-F420-2:alpha-L-glutamate ligase
compare
MMP
_RS05965
+0.2
0.8
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS05990
+0.2
1.4
DsrE family protein
compare
MMP
_RS01105
+0.2
1.5
hypothetical protein
compare
MMP
_RS06480
+0.2
0.9
ABC transporter substrate-binding protein
compare
MMP
_RS00905
+0.2
0.8
phenylacetate--CoA ligase
compare
MMP
_RS06495
+0.2
0.5
YkgJ family cysteine cluster protein
compare
MMP
_RS00285
+0.2
1.0
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS06320
+0.2
1.6
hypothetical protein
compare
MMP
_RS00645
+0.2
1.5
iron ABC transporter permease
compare
MMP
_RS07835
+0.2
0.8
TldD/PmbA family protein
compare
MMP
_RS04120
+0.2
1.7
hypothetical protein
compare
MMP
_RS01965
+0.2
0.9
DUF1616 domain-containing protein
compare
MMP
_RS02175
+0.2
0.2
DUF1611 domain-containing protein
compare
MMP
_RS06550
+0.2
1.2
AMP-binding protein
compare
MMP
_RS00140
+0.2
1.1
DUF192 domain-containing protein
compare
MMP
_RS01095
+0.2
1.2
iron ABC transporter permease
compare
MMP
_RS02020
+0.2
0.7
gamma-glutamylcyclotransferase
compare
MMP
_RS01135
+0.2
1.6
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS01645
+0.2
1.0
SWIM zinc finger family protein
compare
MMP
_RS06500
+0.2
1.3
ATP-binding protein
compare
MMP
_RS05940
+0.2
0.8
corrinoid protein
compare
MMP
_RS08620
+0.2
1.0
flagellar accessory protein FlaH
compare
MMP
_RS00665
+0.2
1.1
hypothetical protein
compare
MMP
_RS08990
+0.2
1.3
hypothetical protein
compare
MMP
_RS07095
+0.2
0.7
PspC domain-containing protein
compare
MMP
_RS05685
+0.2
1.2
winged helix-turn-helix domain-containing protein
compare
MMP
_RS02695
+0.2
1.1
formylmethanofuran dehydrogenase subunit A
compare
MMP
_RS08470
+0.2
1.2
ATP-binding cassette domain-containing protein
compare
MMP
_RS08980
+0.2
1.0
hypothetical protein
compare
MMP
_RS08625
+0.2
1.3
type II/IV secretion system ATPase subunit
compare
MMP
_RS03230
+0.2
1.0
flavodoxin family protein
compare
MMP
_RS08755
+0.2
1.7
sodium:solute symporter family protein
compare
MMP
_RS03540
+0.2
0.8
DUF2178 domain-containing protein
compare
MMP
_RS08130
+0.2
1.5
IGHMBP2 family helicase
compare
MMP
_RS09060
+0.2
1.6
hypothetical protein
compare
MMP
_RS02975
+0.2
0.7
DUF2124 domain-containing protein
compare
MMP
_RS08630
+0.2
1.0
archaellar assembly protein FlaJ
compare
MMP
_RS08325
+0.2
1.4
hypothetical protein
compare
MMP
_RS07640
+0.2
1.0
mechanosensitive ion channel family protein
compare
MMP
_RS03395
+0.2
0.5
hypothetical protein
compare
MMP
_RS07085
+0.2
1.3
ribonuclease HII
compare
MMP
_RS04055
+0.2
0.4
hypothetical protein
compare
MMP
_RS01635
+0.2
1.1
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS03655
+0.2
1.3
DUF1894 domain-containing protein
compare
MMP
_RS08995
+0.2
0.4
hypothetical protein
compare
MMP
_RS00055
+0.2
1.3
DUF2254 domain-containing protein
compare
MMP
_RS01775
+0.2
0.5
TIGR00297 family protein
compare
MMP
_RS06150
+0.2
1.3
hypothetical protein
compare
MMP
_RS04175
+0.2
1.3
permease
compare
MMP
_RS00085
+0.2
1.2
DNA cytosine methyltransferase
compare
MMP
_RS02445
+0.2
1.4
putative DNA binding domain-containing protein
compare
MMP
_RS02940
+0.2
1.4
TatD family hydrolase
compare
MMP
_RS07715
+0.2
1.1
YfcE family phosphodiesterase
compare
MMP
_RS08085
+0.2
1.2
hypothetical protein
compare
MMP
_RS01080
+0.2
0.7
DUF2202 domain-containing protein
compare
MMP
_RS04610
+0.2
0.8
Dabb family protein
compare
MMP
_RS03740
+0.2
0.6
indolepyruvate oxidoreductase subunit beta
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t2 experiments