Experiment set2IT032 for Synechococcus elongatus PCC 7942

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Flask grown to static phase (untill yellowing)

Group: stationary phase
Media: BG11, pH=7.5
Culturing: SynE_ML6, 100ml Flask, Aerobic, at 30 (C), shaken=150 rpm
Growth: about 5.2 generations
By: Ben Rubin on 9/17/2014
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 38 genes in this experiment

SEED Subsystems

Subsystem #Specific
Coenzyme F420 synthesis 2
Arsenic resistance 1
Glutathione: Biosynthesis and gamma-glutamyl cycle 1
Glutathione: Non-redox reactions 1
High affinity phosphate transporter and control of PHO regulon 1
Methylglyoxal Metabolism 1
NAD and NADP cofactor biosynthesis global 1
Nudix proteins (nucleoside triphosphate hydrolases) 1
Phosphate metabolism 1
Pyruvate Alanine Serine Interconversions 1
Rhamnose containing glycans 1
Streptococcal Mga Regulon 1
Two-component regulatory systems in Campylobacter 1
Type IV pilus 1
Utilization of glutathione as a sulphur source 1
dTDP-rhamnose synthesis 1
linker unit-arabinogalactan synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine degradation IV 1 1 1
glutathione degradation (DUG pathway) 2 1 1
methylglyoxal degradation I 3 2 1
methylglyoxal degradation VIII 3 2 1
sucrose biosynthesis III 3 2 1
dTDP-β-D-fucofuranose biosynthesis 4 2 1
dTDP-N-acetylthomosamine biosynthesis 4 2 1
dTDP-6-deoxy-α-D-allose biosynthesis 4 2 1
dTDP-N-acetylviosamine biosynthesis 4 2 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
dTDP-4-O-demethyl-β-L-noviose biosynthesis 5 3 1
dTDP-3-acetamido-α-D-fucose biosynthesis 5 2 1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis 5 2 1
dTDP-α-D-mycaminose biosynthesis 5 2 1
peptido-conjugates in tissue regeneration biosynthesis 17 3 3
γ-glutamyl cycle 6 4 1
dTDP-sibirosamine biosynthesis 6 3 1
dTDP-L-daunosamine biosynthesis 6 3 1
dTDP-D-desosamine biosynthesis 6 2 1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis 6 2 1
leukotriene biosynthesis 6 1 1
dTDP-β-L-olivose biosynthesis 7 3 1
dTDP-β-L-digitoxose biosynthesis 7 3 1
dTDP-β-L-mycarose biosynthesis 7 2 1
sucrose biosynthesis II 8 6 1
dTDP-β-L-4-epi-vancosamine biosynthesis 8 3 1
dTDP-β-L-megosamine biosynthesis 8 3 1
superpathway of methylglyoxal degradation 8 2 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis 9 3 1
dTDP-α-D-forosamine biosynthesis 9 3 1
superpathway of enterobacterial common antigen biosynthesis 10 3 1
O-antigen building blocks biosynthesis (E. coli) 11 10 1
hypoglycin biosynthesis 14 3 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 6 1
superpathway of novobiocin biosynthesis 19 3 1
superpathway of erythromycin biosynthesis 19 2 1
superpathway of megalomicin A biosynthesis 22 3 1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis 23 3 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 1