Experiment set2IT030 for Rhodanobacter denitrificans FW104-10B01

R2A with Chromium(III) Chloride Hexahydrate 0.15625 mM

Group: stress
Media: R2A + Chromium(III) Chloride Hexahydrate (0.15625 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 2/27/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 11 genes in this experiment

For stress Chromium(III) Chloride Hexahydrate in Rhodanobacter denitrificans FW104-10B01

For stress Chromium(III) Chloride Hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	2
Methionine Degradation	2
Peptidoglycan Biosynthesis	2
Campylobacter Iron Metabolism	1
DNA-replication	1
DNA repair, bacterial UvrD and related helicases	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Purine metabolism
• Lipopolysaccharide biosynthesis
• Fructose and mannose metabolism
• Lysine degradation
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Biotin metabolism
• Folate biosynthesis
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan biosynthesis II (staphylococci)	17	11	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	11	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	10	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	10	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	10	1