Experiment set2IT026 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Isoleucine carbon source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_4597 -6.3 -9.6 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_77 -6.1 -5.9 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4596 -6.1 -10.9 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3248 -6.0 -7.2 Probable transmembrane protein compare
Pf6N2E2_64 -6.0 -4.1 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_482 -6.0 -4.1 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf6N2E2_3842 -5.7 -6.8 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_3941 -5.7 -3.9 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_3839 -5.7 -6.7 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_479 -5.6 -6.6 Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) compare
Pf6N2E2_71 -5.6 -7.6 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_4077 -5.6 -3.8 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_5006 -5.5 -11.2 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_477 -5.5 -6.5 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_5176 -5.4 -10.9 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_5175 -5.2 -18.2 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_4047 -5.2 -8.0 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4564 -5.1 -12.3 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_4071 -5.1 -6.6 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_481 -5.1 -7.7 branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) conserved
Pf6N2E2_4825 -5.0 -11.7 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3782 -5.0 -5.7 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_4826 -5.0 -10.7 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_5014 -5.0 -2.2 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_3630 -4.9 -9.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5723 -4.8 -13.6 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_1401 -4.7 -10.9 FIG00955631: hypothetical protein compare
Pf6N2E2_3783 -4.7 -7.7 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4048 -4.7 -8.1 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_5780 -4.6 -12.6 Periplasmic protease compare
Pf6N2E2_4752 -4.6 -14.8 Malate synthase G (EC 2.3.3.9) compare
Pf6N2E2_3253 -4.6 -8.2 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_480 -4.6 -15.6 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) conserved
Pf6N2E2_1147 -4.5 -4.3 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_4453 -4.5 -19.5 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_3170 -4.5 -14.2 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_6064 -4.4 -7.3 2-methylaconitate isomerase compare
Pf6N2E2_4372 -4.4 -7.3 FIG00460773: hypothetical protein compare
Pf6N2E2_5177 -4.3 -10.6 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3610 -4.2 -5.2 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_279 -4.2 -11.7 Isocitrate lyase (EC 4.1.3.1) compare
Pf6N2E2_3932 -4.2 -5.0 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_6063 -4.1 -11.3 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
Pf6N2E2_5160 -3.9 -2.4 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_4803 -3.9 -2.6 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_3252 -3.8 -8.8 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_66 -3.8 -8.8 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_3062 -3.8 -7.1 Membrane-associated zinc metalloprotease compare
Pf6N2E2_5520 -3.8 -9.0 Sensory box histidine kinase compare
Pf6N2E2_2574 -3.8 -3.6 Flagellar synthesis regulator FleN compare
Pf6N2E2_3869 -3.7 -14.0 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_5728 -3.7 -6.5 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_4279 -3.7 -13.5 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_478 -3.6 -4.9 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) compare
Pf6N2E2_1381 -3.5 -7.4 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf6N2E2_3271 -3.4 -3.5 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_3060 -3.3 -3.7 Outer membrane protein H precursor compare
Pf6N2E2_4638 -3.3 -5.4 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_2277 -3.3 -3.8 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_5156 -3.2 -3.8 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_63 -3.2 -4.4 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_1402 -3.2 -7.0 hypothetical protein compare
Pf6N2E2_5430 -3.2 -10.9 Transglycosylase, Slt family compare
Pf6N2E2_3661 -3.1 -7.1 hypothetical protein compare
Pf6N2E2_1400 -3.1 -8.8 Sensory box histidine kinase/response regulator compare
Pf6N2E2_4278 -3.1 -12.9 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_2752 -3.0 -12.4 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_4759 -3.0 -15.1 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_3841 -3.0 -8.0 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_5030 -2.9 -6.3 Transcriptional regulator, AsnC family compare
Pf6N2E2_3940 -2.8 -9.8 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5579 -2.7 -7.0 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_2753 -2.7 -5.6 HtrA protease/chaperone protein compare
Pf6N2E2_2258 -2.7 -12.9 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_5316 -2.6 -3.8 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_4611 -2.6 -4.4 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_3942 -2.5 -9.9 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_1932 -2.4 -6.5 Transcriptional regulator, IclR family compare
Pf6N2E2_2217 -2.3 -6.9 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_50 -2.3 -8.9 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_5242 -2.3 -3.0 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_4277 -2.2 -8.7 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_2588 -2.2 -4.4 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_2756 -2.2 -8.1 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_1146 -2.1 -8.0 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) conserved
Pf6N2E2_3833 -2.1 -5.9 hypothetical protein compare
Pf6N2E2_3460 -2.0 -2.9 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_3349 -2.0 -2.2 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_4416 -1.9 -3.4 Tyrosine recombinase XerC compare
Pf6N2E2_4362 -1.9 -4.5 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_5255 -1.8 -9.6 Outer membrane stress sensor protease DegS compare
Pf6N2E2_2218 -1.8 -5.4 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_4418 -1.8 -1.3 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_4160 -1.8 -2.2 Cytochrome c5 compare
Pf6N2E2_4072 -1.7 -4.4 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_3984 -1.7 -9.3 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_6062 -1.7 -6.1 2-methylcitrate synthase (EC 2.3.3.5) (from data) compare
Pf6N2E2_180 -1.6 -3.7 Transcriptional regulator, MerR family compare
Pf6N2E2_2186 -1.6 -6.7 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_623 -1.6 -1.4 FIG00954674: hypothetical protein compare
Pf6N2E2_3834 -1.6 -5.3 Lipoprotein NlpD compare
Pf6N2E2_5219 -1.6 -8.0 FIG140336: TPR domain protein compare
Pf6N2E2_625 -1.6 -5.8 MoxR-like ATPase in aerotolerance operon compare
Pf6N2E2_4009 -1.6 -8.4 GGDEF domain protein compare
Pf6N2E2_4585 -1.5 -10.1 Cell division protein FtsX compare
Pf6N2E2_5553 -1.5 -8.1 Quinolinate synthetase (EC 2.5.1.72) compare
Pf6N2E2_2746 -1.5 -6.4 Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor compare
Pf6N2E2_1143 -1.5 -5.7 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases conserved
Pf6N2E2_5345 -1.5 -6.5 LppC putative lipoprotein compare
Pf6N2E2_4414 -1.4 -4.4 FIG00953808: hypothetical protein compare
Pf6N2E2_5771 -1.4 -5.3 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_2074 -1.4 -1.8 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_3885 -1.4 -3.7 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_4511 -1.4 -7.5 glutamine synthetase family protein compare
Pf6N2E2_4584 -1.4 -2.3 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
Pf6N2E2_624 -1.4 -5.4 hypothetical protein PA3071 compare
Pf6N2E2_1138 -1.3 -5.4 Transcriptional regulator, AraC family conserved
Pf6N2E2_280 -1.3 -1.8 hypothetical protein compare
Pf6N2E2_620 -1.2 -6.8 BatD compare
Pf6N2E2_4276 -1.2 -4.3 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_2509 -1.2 -2.9 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_4206 -1.2 -2.1 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_622 -1.2 -3.9 BatA (Bacteroides aerotolerance operon) compare
Pf6N2E2_621 -1.2 -4.7 TPR domain protein in aerotolerance operon compare
Pf6N2E2_3835 -1.2 -3.2 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_3074 -1.2 -2.1 FIG00960973: hypothetical protein compare
Pf6N2E2_2586 -1.2 -2.0 Hpt domain protein compare
Pf6N2E2_4067 -1.2 -7.0 Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) compare
Pf6N2E2_3461 -1.1 -1.6 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_4353 -1.1 -5.0 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_3282 -1.1 -2.0 FIG005080: Possible exported protein compare
Pf6N2E2_4074 -1.1 -6.3 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_4993 -1.0 -2.2 Gamma-glutamyl phosphate reductase (EC 1.2.1.41) compare
Pf6N2E2_5806 -1.0 -3.2 hypothetical protein compare
Pf6N2E2_4187 -1.0 -2.2 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_1596 -1.0 -1.8 Periplasmic nitrate reductase component NapD compare
Pf6N2E2_2922 -1.0 -3.0 hypothetical protein compare
Pf6N2E2_5252 -1.0 -1.9 FIG00956396: hypothetical protein compare
Pf6N2E2_3095 -1.0 -1.2 hypothetical protein compare
Pf6N2E2_3346 -1.0 -4.8 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_5086 -1.0 -1.2 hypothetical protein compare
Pf6N2E2_6100 -1.0 -2.0 tRNA-Val-TAC compare
Pf6N2E2_51 -1.0 -4.3 hypothetical protein compare
Pf6N2E2_84 -1.0 -2.0 hypothetical protein compare
Pf6N2E2_1705 -0.9 -1.5 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_4075 -0.9 -3.2 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_493 -0.9 -3.6 YD repeat protein compare
Pf6N2E2_3578 -0.9 -7.0 Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1) compare
Pf6N2E2_3762 -0.9 -4.2 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_1821 -0.9 -1.6 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_5469 -0.9 -4.7 L-lysine permease compare
Pf6N2E2_3613 -0.9 -5.1 GTP-binding protein HflX compare
Pf6N2E2_6061 -0.9 -4.0 Methylisocitrate lyase (EC 4.1.3.30) (from data) conserved
Pf6N2E2_4162 -0.9 -1.9 DNA polymerase I (EC 2.7.7.7) compare
Pf6N2E2_4272 -0.9 -2.9 putative membrane protein compare
Pf6N2E2_3612 -0.9 -1.9 HflK protein compare
Pf6N2E2_3577 -0.9 -5.6 ABC transporter for L-leucine/L-isoleucine/D-alanine, ATPase component 1 LivG (from data) compare
Pf6N2E2_3580 -0.8 -6.5 Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1) compare
Pf6N2E2_127 -0.8 -1.0 AttE component of AttEFGH ABC transport system compare
Pf6N2E2_827 -0.8 -1.9 probable dioxygenase compare
Pf6N2E2_1056 -0.8 -0.6 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_3251 -0.8 -5.8 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_5593 -0.8 -2.5 Leucyl-tRNA synthetase compare
Pf6N2E2_3579 -0.8 -6.2 High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1) compare
Pf6N2E2_449 -0.8 -2.8 Lactoylglutathione lyase (EC 4.4.1.5) compare
Pf6N2E2_4163 -0.8 -2.9 FIG00953549: hypothetical protein compare
Pf6N2E2_4817 -0.8 -6.6 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_1137 -0.8 -4.9 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_1723 -0.8 -1.4 Transcriptional regulator, TetR family compare
Pf6N2E2_1875 -0.8 -1.9 Protoporphyrinogen oxidase (EC 1.3.3.4) compare
Pf6N2E2_4773 -0.8 -1.0 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_2964 -0.8 -2.9 FIG006285: ICC-like protein phosphoesterase compare
Pf6N2E2_5989 -0.8 -6.2 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_302 -0.8 -2.9 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_5936 -0.8 -1.5 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) compare
Pf6N2E2_146 -0.7 -1.5 Transcriptional regulator, MarR family compare
Pf6N2E2_70 -0.7 -1.9 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_4478 -0.7 -3.1 FIG019278: hypothetical protein compare
Pf6N2E2_2073 -0.7 -4.0 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_2340 -0.7 -2.3 Transcriptional regulator, GntR family compare
Pf6N2E2_1812 -0.7 -2.6 peptide ABC transporter, ATP-binding protein compare
Pf6N2E2_5260 -0.7 -4.3 Phosphate acetyltransferase (EC 2.3.1.8) compare
Pf6N2E2_3326 -0.7 -1.0 hypothetical protein compare
Pf6N2E2_417 -0.7 -1.8 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf6N2E2_3096 -0.7 -0.9 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_2473 -0.7 -1.0 Major outer membrane lipoprotein I compare
Pf6N2E2_5956 -0.7 -3.2 FIG004453: protein YceG like compare
Pf6N2E2_2919 -0.7 -1.8 FIG00955830: hypothetical protein compare
Pf6N2E2_3616 -0.7 -4.2 DNA mismatch repair protein MutL compare
Pf6N2E2_3285 -0.7 -1.1 Rod shape-determining protein MreD compare
Pf6N2E2_2501 -0.7 -1.6 hypothetical protein compare
Pf6N2E2_2926 -0.7 -3.8 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data) compare
Pf6N2E2_5536 -0.7 -0.8 Holliday junction DNA helicase RuvA compare
Pf6N2E2_3828 -0.7 -2.1 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf6N2E2_4999 -0.7 -3.4 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf6N2E2_3199 -0.7 -3.4 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_4527 -0.7 -1.9 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf6N2E2_3201 -0.7 -2.0 Transcriptional regulator, MerR family compare
Pf6N2E2_3725 -0.7 -1.1 FIG085779: Lipoprotein compare
Pf6N2E2_2496 -0.7 -1.4 FIG00956575: hypothetical protein compare


Specific Phenotypes

For 6 genes in this experiment

For carbon source L-Isoleucine in Pseudomonas fluorescens FW300-N2E2

For carbon source L-Isoleucine across organisms