Experiment set2IT026 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Isoleucine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Isoleucine (20 mM), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/9/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 6 genes in this experiment

For carbon source L-Isoleucine in Pseudomonas fluorescens FW300-N2E2

For carbon source L-Isoleucine across organisms

SEED Subsystems

Subsystem #Specific
Isoleucine degradation 3
Valine degradation 3
Leucine Degradation and HMG-CoA Metabolism 2
Acetyl-CoA fermentation to Butyrate 1
Anaerobic respiratory reductases 1
Butanol Biosynthesis 1
Isobutyryl-CoA to Propionyl-CoA Module 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
ethanol degradation II 3 3 1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 1
superpathway of acetate utilization and formation 3 3 1
ethanol degradation IV 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation III 3 2 1
chitin deacetylation 4 2 1
L-isoleucine degradation I 6 5 1
pyruvate fermentation to butanol II (engineered) 6 4 1
propanoate fermentation to 2-methylbutanoate 6 4 1
superpathway of bitter acids biosynthesis 18 3 3
colupulone and cohumulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
L-valine degradation I 8 6 1
valproate β-oxidation 9 7 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
cis-geranyl-CoA degradation 9 3 1
pyruvate fermentation to hexanol (engineered) 11 7 1
2-methyl-branched fatty acid β-oxidation 14 11 1
1-butanol autotrophic biosynthesis (engineered) 27 20 1