Experiment set2IT026 for Pedobacter sp. GW460-11-11-14-LB5

R2A with Aluminum chloride hydrate 1 mM

Group: stress
Media: R2A + Aluminum chloride hydrate (1 mM)
Culturing: Pedo557_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.7 generations
By: Adam on 17-May-17
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1758 B1

Specific Phenotypes

For 17 genes in this experiment

For stress Aluminum chloride hydrate in Pedobacter sp. GW460-11-11-14-LB5

For stress Aluminum chloride hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	2
Cobalt-zinc-cadmium resistance	1
Lysine Biosynthesis DAP Pathway	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Peptidoglycan Biosynthesis	1
Threonine and Homoserine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Folate biosynthesis
• Biosynthesis of plant hormones
• Fructose and mannose metabolism
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• gamma-Hexachlorocyclohexane degradation
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine ribonucleosides salvage I	3	3	2
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
diethylphosphate degradation	2	1	1
L-homoserine biosynthesis	3	3	1
dipicolinate biosynthesis	4	3	1
spermidine biosynthesis II	4	2	1
L-methionine biosynthesis IV	4	2	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
superpathway of pyrimidine ribonucleosides salvage	10	9	2
ectoine biosynthesis	5	2	1
superpathway of L-threonine biosynthesis	6	6	1
norspermidine biosynthesis	6	3	1
L-lysine biosynthesis VI	7	6	1
L-lysine biosynthesis III	7	6	1
3-dehydroquinate biosynthesis II (archaea)	7	3	1
cremeomycin biosynthesis	7	2	1
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
superpathway of polyamine biosynthesis III	8	3	1
grixazone biosynthesis	8	2	1
L-lysine biosynthesis II	9	8	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
L-lysine biosynthesis I	9	7	1
nucleoside and nucleotide degradation (archaea)	10	4	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
chorismate biosynthesis II (archaea)	12	8	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	14	1
aspartate superpathway	25	21	1
platensimycin biosynthesis	26	6	1