Experiment set2IT025 for Acidovorax sp. GW101-3H11

LB with copper (II) chloride dihydrate 2 mM

Group: stress
Media: LB + copper (II) chloride dihydrate (2 mM)
Culturing: acidovorax_3H11_ML3a, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 5/11/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 6 genes in this experiment

For stress copper (II) chloride dihydrate in Acidovorax sp. GW101-3H11

For stress copper (II) chloride dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Biotin biosynthesis	1
HMG CoA Synthesis	1
Leucine Degradation and HMG-CoA Metabolism	1
Lipoic acid metabolism	1
Polyhydroxybutyrate metabolism	1
Soluble cytochromes and functionally related electron carriers	1
n-Phenylalkanoic acid degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Geraniol degradation
• Fatty acid metabolism
• Tyrosine metabolism
• alpha-Linolenic acid metabolism
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Propanoate metabolism
• Ethylbenzene degradation
• Butanoate metabolism
• Limonene and pinene degradation
• Caprolactam degradation
• Alkaloid biosynthesis II
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
long-chain fatty acid activation	1	1	1
acetate formation from acetyl-CoA (succinate)	1	1	1
γ-linolenate biosynthesis II (animals)	2	1	1
linoleate biosynthesis II (animals)	2	1	1
pyruvate fermentation to acetate V	3	3	1
pyruvate fermentation to acetate VI	3	2	1
3-methyl-branched fatty acid α-oxidation	6	3	2
oleate biosynthesis I (plants)	3	1	1
alkane biosynthesis II	3	1	1
phytol degradation	4	3	1
phosphatidylcholine acyl editing	4	2	1
long chain fatty acid ester synthesis (engineered)	4	2	1
wax esters biosynthesis II	4	1	1
sporopollenin precursors biosynthesis	18	4	4
octane oxidation	5	3	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
fatty acid salvage	6	5	1
stearate biosynthesis II (bacteria and plants)	6	5	1
stearate biosynthesis IV	6	4	1
6-gingerol analog biosynthesis (engineered)	6	3	1
stearate biosynthesis I (animals)	6	1	1
ceramide degradation by α-oxidation	7	2	1
succinate fermentation to butanoate	7	2	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
capsaicin biosynthesis	7	1	1
2-deoxy-D-ribose degradation II	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
TCA cycle VII (acetate-producers)	9	7	1
anaerobic energy metabolism (invertebrates, mitochondrial)	10	7	1
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	1	1
superpathway of anaerobic energy metabolism (invertebrates)	17	14	1
superpathway of fatty acids biosynthesis (E. coli)	53	49	2
palmitate biosynthesis III	29	28	1
oleate β-oxidation	35	29	1