Experiment set2IT023 for Synechococcus elongatus PCC 7942
Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light
Group: light-darkMedia: BG11, pH=7.5
Culturing: SynE_ML6, 100ml Flask, Aerobic, at 30 (C), shaken=150 rpm
Growth: about 6.5 generations
By: Ben Rubin on 9/17/2014
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)
Specific Phenotypes
For 26 genes in this experiment
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nucleotide sugars metabolism
- Fructose and mannose metabolism
- Oxidative phosphorylation
- Citrate cycle (TCA cycle)
- Pentose phosphate pathway
- Ubiquinone and menaquinone biosynthesis
- Methionine metabolism
- Tryptophan metabolism
- Cyanoamino acid metabolism
- Starch and sucrose metabolism
- Other glycan degradation
- Aminosugars metabolism
- Glycosaminoglycan degradation
- Lipopolysaccharide biosynthesis
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Reductive carboxylate cycle (CO2 fixation)
- Riboflavin metabolism
- Nicotinate and nicotinamide metabolism
- Porphyrin and chlorophyll metabolism
- Terpenoid biosynthesis
- Carotenoid biosynthesis - General
- Phenylpropanoid biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: